The c-myc coding DNA sequences of cyprinids （Teleostei： Cypriniformes）： Implications for phylogeny

The family Cyprinidae is one of the largest fish families in the world, which is widely distributed in East Asian, with obvious difference in characteristic size among species. The phylogeneUc analysis of cyprinid taxa based on the functionally important genes can help to understand the speciation and functional divergence of the Cyprinidae. The c-myc gene is an important gene regulating individual growth. In the present study, the sequence variations of the cyprinid c-myc gene and their phylogenetic significance were analyzed. The 41 complete sequences of the c-myc gene were obtained from cyprinids and outgroups through PCR amplification and clone. The coding DNA sequences of the c-myc gene were used to infer molecular phylogenetic relationships within the Cyprinidae. Myxocyprinus asiaticus （Catostomidae）, Misgurnus anguillicaudatus （CobiUdae） and Hemimyzon sinensis （Homalopteridae） were assigned to the outgroup taxa. Phylogenetic analyses using maximum parsimony （MP）, maximum likelihood （ML）, and Bayesian retrieved similar topology. Within the Cyprinidae, Leuciscini and Barbini formed the monophyletic lineage respectively with high nodal supports. Leuciscini comprises Xenocyprinae, CuItrinae, East Asian species of Leuciscinae and Danioninae, Gobioninae and Acheilognathinae, and Barbini contains Schizothoracinae, Barbinae, Cyprininae and Labeoninae. Danio rerio, D. myersi and Rasbora trilineata were supposed to separate from Leuciscinae and Barbini and to form another lineage. The positions of some Danioninae species were still unresolved. Analyses of both amino acid variation with parsimony information and two high variation regions indicated that there is no correlation between variations of single amino acid or high variation regions and characteristic size of cyprinids. In addition, the species with smaller size were usually found to be basal within clades in the tree, which might be the results of the adaptation to the primitive ecology and survival pressure.     

Experimental investigation of incremental reactivity of di-tert-butyl peroxide

Large quantities of di-tert-butyl peroxide (DTBP) have been emitted into the troposphere due to human activities. Its role in the atmospheric photochemical reaction has not been understood. This study presents the results of the photochemical reactions of DTBP and NOx, which have been simulated in a self-made smog chamber under the temperature of (29±1)℃. Both the wall decays of ozone and NO2 could be neglected, compared to the results in simulative experiments. The effective intensity of UV light used in the experiments was 1.28×10-3 s-1, which was expressed by the rate constant of NO2 photolysis in purified air. The reaction mechanism was proposed according to our results and reports of other researchers. The maximum values of incremental reactivity (IR) in the three simulative ex- periments were 9.53×10-2, 5.23×10-2 and 3.78×10-2, respectively. The incremental reactivity decreased with the increase of initial concentrations of DTBP. The IR value of DTBP obtained in this study was comparable to that of acetylene reported in our previous research.     

Reconstruction of linkage maps in the distorted segregation populations of backcross, doubled haploid and recombinant inbred lines

Non-Mendelian segregation of markers, known as distorted segregation, is a common biological phenomenon. Although segregation distortion affects the estimation of map distances and the results of quantitative trait loci (QTL) mapping, the effects of distorted markers are often ignored in the construction of linkage maps and in QTL mapping. Recently, we have developed a multipoint method via a Hidden Markov chain method to reconstruct linkage maps in an F2 population that corrects for bias of map distances between distorted markers. In this article, the method is extended to cover backcross, doubled haploid and recombinant inbred line (RIL) populations. The results from simulated experiments show that: (1) the degree that two linked segregation distortion loci (SDL) affect the estimation of map distances increases as SDL heritability and interval length between adjacent markers increase, whereas sample size has little effect on the bias; (2) two linked SDL result in the underesti- mation of linkage distances for most cases, overestimation for an additive model with opposite additive effects, and unbiased estimation for an epistatic model with negative additive-by-additive effects; (3) the proposed method can obtain the unbiased estimation of linkage distance. This new method was applied to a rice RIL population with severely distorted segregation to reconstruct the linkage maps, and a bootstrap method was used to obtain 95% confidence intervals of map distances. The results from real data analysis further demonstrate the utility of our method, which provides a foundation for the inheritance analysis of quantitative and viability traits.     

Plasma sheet stretching accompanied by field aligned energetic ion fluxes observed by the NUADU instrument aboard TC-2

The NUADU (NeUtral Atom Detector Unit) instrument aboard TC-2 recorded 4л solid angle images of charged particles (E >180 keV) spiraling around the magnetic field lines in the near-Earth plasma sheet (at ~ -7 RE, equatorial dawn-to-night side) during a geomagnetic storm (Dst =-219 nT) on August 24, 2005. Energetic ion beam events characterized by symmetrical, ring-like, solid angle distributions around ambient magnetic field lines were observed during a 34-minute traversal of the plasma sheet by the TC-2 spacecraft. Also, observations during these multiple crossings of the plasma sheet were monitored by the magnetometer experiment (FGM) aboard the same spacecraft. During each crossing, a whistler-mode chorus enhancement was observed in the anisotropic area by the TC-2 low frequency electromagnetic wave detector (LFEW/TC-2) at a frequency just above that of the local lower hybrid wave. A comparison of the ion pitch angle distribution (PAD) map with the ambient magnetic field shows that an enhancement in the field aligned energetic ion flux was accompanied by tailward stretching of the magnetic field lines in the plasma sheet. In contrast, the perpendicular ion-flux enhancement was accompanied by a signature indicating the corresponding shrinkage of the magnetic field lines in the plasma sheet. Since both parallel ion-flux and perpendicular ion-flux enhancements occurred intermittently, the data were interpreted to imply a dynamical, oscillatory process of the magnetic field line (stretching and shrinking) in the near-Earth plasma sheet, which might have acted to help establish an interaction region in this area which would support continuous aurora-substorm triggering during the ongoing magnetic storm. The whistler-mode chorus may have been produced due to ion gyro-resonance during particle pitch angle diffusion after the plasma sheet compression.     

基于Qmail的邮件即时通告系统分析与设计

传统电子邮件系统收发邮件的工作模式中，不能提供邮件到达即时通知，导致邮件利用延迟。以Qmail邮件系统内核为平台，通过对其基本功能结构进行分析，结合SMS短信通讯平台，设计并实现了邮件到达即时通告。     

Carboxymethytl Pachymaram Up-Regulates Dendritic Cell＇s Function in Hepatitis B Virus Transgenic Mice

The effect of carboxymethytl pachymaram (CMP) on the function of dendritic cells(DCs) derived from spleens of hepatitis B virus transgenic mice are studied in vitro. The phenotypes of DCs are tested by flow cytometry (FCM), cytokines measured by ELISA. The expression of DCs’ phenotypes in HBV transgenic mice are low (CD80⁺CD11c⁺:59.12±11.53 vs 9.60±4.53, p<0.01; CD80⁺ MHC-II⁺: 44.86±12.31 vs 9.80±5.72, p<0.01, normal mice vs HBV transgenic mice), the ability of DCs stimulating T lymphocytes proliferation decreases (0.37±0.11 vs 0.20±0.11, p<0.05, normal mice vs HBV transgenic mice), levels of IL-12 and IFN-γ decrease whereas the level of IL-10 increases; CMP can enhance DCs’ ability of stimulating T lymphocytes proliferation, facilitate the secretion of IL-12 and IFN-γ, inhibit the secretion of IL-10, thus up regulates DCs function. The results show a good prospective use of CMP on the treatment of chronic hepatitis B. Biography: HOU Anji (1963–), male, Ph.D. candidate, Associate professor, research direction: clinical virology.     

Numerical simulation on the evolution of sediment waves caused by turbidity currents

Interest in the forming mechanism of sediment waves increases recently because of its significance on submarine engineering, sedimentary dynamics and hydrocarbon reservoir prediction in deep water. In this paper, the time-averaged continuity equations and Reynolds-averaged Navier-Stokes equations are applied in the numerical simulation of fluid dynamics. The modeling results are used to illuminate the effects of topography on turbidity current and explore the origin of submarine sediment waves. The research results show that (1) deposition occurs firstly at the lower ramp due to the deceleration of fluid, increase of density, loss of flow capacity and longer duration of flow passage; (2) density increase at the upslope due to the local jam results in velocity decrease and pressure increase; (3) sediment waves begin to be formed and migrated toward upstream in an area far away from the source with in- crease of the turbidity events; (4) deposition becomes more slowly with decrease of grain sizes, but the shape and sequences of these deposits are controlled by topography, not grain size.     

Fabrication and superconducting properties of nano-SiC doped MgB2 tapes

Nano-SiC doped MgB2 tapes were prepared by the in situ powder-in-tube method. Heat treatment was performed at 650℃ for 1 h. XRD data indicate that SiC particles had reacted with the MgB2 during sintering process. MgB2 core seemed to be denser after SiC doping, and the critical temperature was slightly depressed. The critical current density Jc of the SiC doped tapes was significantly enhanced in magnetic fields up to 14 T compared to the undoped ones. For the 5% SiC doped samples, Jc was in- creased by a factor of 32 at 4.2 K, 10 T. The enhancement of Jc-B properties in SiC doped MgB2 tapes is considered to be due to the enhancement of grain linkages and the introduction of effective flux pining centers. The substitution of B by C in MgB2 grains is thought to be the main reason for the improve- ment of the flux pinning ability in SiC doped MgB2 tapes.