步进电机细分技术在经济型数控机床中的应用

本文叙述了步进电机细分原理及微机控制系统，并将其应用于经济型数控铣床的横向进给伺服系统，有效提高了开环数控系统的定位精度。     

用酉几何构作笛卡尔认证码

利用有限域上的酉几何构作了一个笛卡尔认证码，并计算了它们的参数。此外，假定根据均匀概率分布选择编码规则，计算了模仿攻击与替换攻击成功的概率的最大值Ｐ１与Ｐｓ。     

电视逆程数据广播的原理与应用

详细介绍了电视逆程资源用于数据广播的原理与现状，探讨了电视逆程资源新的利用方式，计算了电视逆程进行数据广播的潜力。利用电视逆程作为交互电视的先导，采用了一种简单的设计方案，满足了图文电视节目的视频点播。     

Transgenesis in fish

Gene transfer into fish embryo is being performed in several species (trout, salmon, carps, tilapia, medaka, goldfish, zebrafish, loach, catfish, etc.). In most cases, pronuclei are not visible and microinjection must be done into the cytoplasm of early embryos. Several million copies of the gene are generally injected. In medaka, transgenesis was attempted by injection of the foreign gene into the nucleus of oocyte. Several reports indicate that the injected DNA was rapidly replicated in the early phase of embryo development, regardless of the origin and the sequence of the foreign DNA. The survival of the injected embryos was reasonably good and a large number reached maturity. The proportion of transgenic animals ranged from 1 to 50% or more, according to species and to experimentators. The reasons for this discrepancy have not been elucidated. In all species, the transgenic animals were mosaic. The copy number of the foreign DNA was different in the various tissues of an animal and a proportion lower than 50% of F1 offsprings received the gene from their parents. This suggests that the foreign DNA was integrated into the fish genome at the two cells stage or later. An examination of the integrated DNA in different cell types of an animal revealed that integration occurred mainly during early development. The transgene was found essentially unrearranged in the fish genome of the founders and offsprings. The transgenes were therefore stably transmitted to progeny in a Mendelian fashion. Southern blot analysis revealed the presence of possible junction fragments and also of minor bands which may result from a rearrangement of the injected DNA. In all species, the integrated DNA appeared mainly as random end-to-end concatemers. In adult trout blood cells, a small proportion of the foreign DNA was maintained in the form of non-integrated concatemers, as judged by the existence of end fragments. The transgenes were generally only poorly expressed. The majority of the injected gene constructs contained essentially mammalian or higher vertebrates sequences. The comparison of the expression efficiency of these constructs in transfected fish and mammalian cells indicates that some of the mammalian DNA sequences are most efficiently understood by the fish cell machinery. Chloramphenicol acetyl transferase gene under the control of promoters from Rous sarcoma virus, and human cytomegalovirus, was expressed in several tissues of transgenic fish. Chicken delta-crystallin gene was expressed in several tissues of transgenic fish.(ABSTRACT TRUNCATED AT 400 WORDS)     

理工科院校法学专业走出就业困境之策略探析

经过几年的扩招之后，各理工科院校培养出的法学专业毕业生已经开始陆续走向市场，从各理工科院校所统计的数据来看，法学专业毕业生的就业问题已显得十分突出。突出法学教育在理工科院校的专业特色，多方面强化学生的实践能力是理工科院校法学专业走出就业困境的必由之路。各理工科院校应理性地面对目前的艰难与困境，充分依据各自的资源来追求自己在法学教育上的比较优势。     

Chromosomal localization of a unique gene by non-autoradiographic in situ hybridization

During the past few years, several methods have been developed for the detection of specific nucleic acid sequences by in situ hybridization using non-radioactive labels such as fluorochromes, cytochemically detectable enzymes and electron-dense markers. These methods are preferable to autoradiography in terms of speed of performance and topological resolution. Their limited sensitivity, however, has so far restricted their use to the detection of repeated sequences. Here we report single gene detection with a procedure using 2-acetylaminofluorene (AAF)-modified probes, immunoperoxidase cytochemistry and reflection-contrast microscopy. We confirmed the autoradiographic data on the localization of the human thyroglobulin (Tg) gene to the distal end of the long arm of chromosome 8. A mixture of cosmid cHT2-derived subclones of the 3' part of the Tg gene, 22.3 kilobase pairs (kbp) in total, was used as a hybridization probe. This procedure can be used to map other unique sequences, if genomic clones are available from which clones with an appropriate amount of inserts can be isolated.     

集成电路高精度高速度在线电阻检测

本文提出的具有粗、精测双回路的测试系统,可兼顾电阻在线检测过程中的高精度与高速度问题。该系统各器件间的信息交换满足IEEE-488标准。