化学发光自显影免疫印迹法（Western Blotting）检测绒山羊皮肤组织中催乳素受体蛋白

采集不同季节绒山羊皮肤组织样品,提取总蛋白,经聚丙烯酰胺凝胶电泳,将电泳结果转移到醋酸纤维薄膜,然后与催乳素抗体结合、辣根过氧化物酶标记二抗结合,后者作用于化学发光底物,暗室中置X-光胶片上自显影数分钟,经显影、定影,得到黑色曝光斑,根据胶片上曝光斑的位置确定出催乳素受体的分子量为87kD。根据不同时期样品黑斑的大小得出各时期催乳素受体表达量与绒毛生长趋势是一致的。     

道路交通驾驶员差异性分类定量研究

驾驶员个体差异是影响城市道路交通运营、效率及安全的一个重要因素之一。为了实现对驾驶员差异性的影响分析,采用驾驶模拟舱研究平台对驾驶员样本进行归类。研究分别选取了反映驾驶员特征的静态驾驶适性和动态驾驶适性两类,共计15个指标。采用因子分析的方法将相关指标归纳为驾驶员的5个类因子,并通过主成份分析的方法找出每类因子中的主导指标,并以此指标作为聚类分析的输入变量。结果表明聚类分析得到的三类驾驶员具有明显的各自特征。     

播种方式对紫花苜蓿＋无芒雀麦人工草地土壤剖面养分分布的影响

采用田间调查取样和室内分析相结合的方法,2007年在内蒙古民族大学农学院试验农场以2年生单播紫花苜蓿、单播无芒雀麦、隔行混播和同行混播人工草地为研究对象,采用分层取样法研究了不同播种方式对土壤理化性质的影响,结果表明：在牧草生长期间单播无芒雀麦草地土壤容重最小,紫花苜蓿草地土壤容重最大;单播无芒雀麦、隔行混播和同行混播草地0～20cm土层有机质含量大于单播紫花苜蓿草地,20～30cm不显著,30～40cm单播紫花苜蓿草地显著增加,分别是隔行混播草地、同行混播和单播无芒雀麦草地的1.11、1.72和1.94倍;速效N单播无芒雀麦草地最低,速效P、速效K单播紫花苜蓿草地最低.     

基于CVaR的能源互补联合系统优化配置模型研究

由于风电、光电出力具有不确定性,因此会引起电网频率的较大波动,严重影响电能质量.为了保证含有新能源电力系统的稳定运行,特提出风光水储联合调度优化模型.首先,建立含新能源的电力系统优化调度数学模型,该模型追求系统机组耗量最小.在进行含新能源发电的电力系统优化调度中,可被用来调度的出力实际为满足负荷功率后,扣除新能源输出功率的那部分火电机组出力.算例结果表明,本文采取CVaR具有良好的性能;风电与光伏清洁能源机组并入系统中增加了日前调度的不确定性风险,但通过CVaR评估计算系统风险,采取有效的措施会使系统发电成本减少与碳排放量减少.     

Oxidative stress triggers neuronal caspase-independent death: Endonuclease G involvement in programmed cell death-type III

To characterize neuronal death, primary cortical neurons (C57/Black 6 J mice) were exposed to hydrogen peroxide (H₂O₂) and staurosporine. Both caused cell shrinkage, nuclear condensation, DNA fragmentation and loss of plasma membrane integrity. Neither treatment induced caspase-7 activity, but caspase-3 was activated by staurosporine but not H₂O₂. Each treatment caused redistribution from mitochondria of both endonuclease G (Endo G) and cytochrome c. Neurons knocked down for Endo G expression using siRNA showed reduction in both nuclear condensation and DNA fragmentation after treatment with H₂O₂, but not staurosporine. Endo G suppression protected cells against H₂O₂-induced cell death, while staurosporine-induced death was merely delayed. We conclude that staurosporine induces apoptosis in these neurons, but severe oxidative stress leads to Endo G-dependent death, in the absence of caspase activation (programmed cell death-type III). Therefore, oxidative stress triggers in neurons a form of necrosis that is a systematic cellular response subject to molecular regulation. Electronic supplementary material  The online version of this article (doi:) contains supplementary material, which is available to authorized users.     

Influence of the carbohydrate moiety on the growth inhibitory activity and adhesiveness of 3T3 cell plasma membranes

Treatment of 3T3 cell plasma membranes with glycosidase enzymes decreased their ability to inhibit cell growth and also decreased their binding to 3T3 cells. This suggests that carbohydrate is required for complete function of inhibitory activity and that inhibition is associated with membrane adhesion.