A transgenic insertion upstream of sox9 is associated with dominant XX sex reversal in the mouse

In most mammals, male development is triggered by the transient expression of the Y-chromosome gene, Sry, which initiates a cascade of gene interactions ultimately leading to the formation of a testis from the indifferent fetal gonad. Several genes, in particular Sox9, have a crucial role in this pathway. Despite this, the direct downstream targets of Sry and the nature of the pathway itself remain to be clearly established. We report here a new dominant insertional mutation, Odsex (Ods), in which XX mice carrying a 150-kb deletion (approximately 1 Mb upstream of Sox9) develop as sterile XX males lacking Sry. During embryogenesis, wild-type XX fetal gonads downregulate Sox9 expression, whereas XY and XX Ods/+ fetal gonads upregulate and maintain its expression. We propose that Ods has removed a long-range, gonad-specific regulatory element that mediates the repression of Sox9 expression in XX fetal gonads. This repression would normally be antagonized by Sry protein in XY embryos. Our data are consistent with Sox9 being a direct downstream target of Sry and provide genetic evidence to support a general repressor model of sex determination in mammals.     

美国东北部早熟和晚熟玉米杂交种年际间产量稳定性

玉米是美国东北部奶牛业的主要能量来源，杂交种的选择是提高玉米产量、降低生产成本的关键和前提。连续5年在美国麻省大学农学实验站的典型砂壤土耕地上进行试验，结合当地气象部门提供的生育期间玉米的生长期生长度日数和降雨量，对3个早熟杂交种和3个晚熟杂交种的产量进行了年际间稳定性比较。结果表明：产量与年际间气候条件无关，无论是青贮饲料产量还是穗棒产量，晚熟品种的产量均高于早熟品种（2001年杂交种E409L）。5年平均晚熟品种的青贮饲料产量和穗棒产量分别比早熟品种高出10．9％和8．0％。相同年份品种间产量存在差异，同一品种年际间产量差异显著。低温年份，相对冷凉的生育前期和温热的生育后期不利于美国东北部玉米生产。生育期间较高的降雨量有利于青贮产量的形成。选择种植晚熟品种是该区农民提高玉米产量、降低生产成本的有效途径。只有热量多且雨量充沛的年份才有利于玉米产量潜力的挖掘。有关某种玉米基因型对当地气候适应性方面的知识，将有利于育种者选择有价值的种质资源。表6，参17。     

酚与4,4′-联吡啶π-π叠加作用的核磁氢谱研究

通过核磁氢谱法研究酚类化合物与4,4′-联吡啶季铵盐π-π叠加作用,研究了吡啶环上α-H,β-H及氧乙基上的α′-H,β′-H的化学位移值及其改变的情况.结果表明,β-萘酚、α-萘酚、对苯二酚、间苯二酚及苯酚使上述氢的化学位移降低,其化学位移降低次序为:β萘酚>α-萘酚>对苯二酚、间苯二酚>苯酚.而8-羟基喹啉和间硝基苯酚导致上述氢的化学位移稍升高.     

灌孔混凝土砌块砌体受压性能研究

通过对灌孔混凝土砌块砌体中混凝土芯柱三向受压应力状态进行分析,提出了适用于灌孔混凝土受压弹性模量的计算方法.同时,基于大量的试验结果,提出了适用于灌孔混凝土砌块砌体的泊松比、峰值应变、极限应变等参数,并引入到Domingo J.Carreira等提出的混凝土本构模型中,得出了适用于灌孔混凝土砌块砌体受压的应力-应变全曲线公式.对比结果表明,计算值与试验结果吻合良好,可供灌孔混凝土砌块砌体结构设计和研究参考.     

Malarial parasites complete sporogony in axenic mosquitoes

Summary To obtain sporogonic stages of malaria free from microbial contaminants for in vitro studies,Anopheles stephensi were reared under sterile conditions using a mosquito cell line as larval food. The adult females, kept in sterile humidified containers and allowed to engorge on parasitemic hamsters, supported the sporogonic development of the rodent malarial parasitePlasmodium berghei. In 10 experiments, the proportion of infected mosquitoes varied from 0 to 92%, and the geometric mean number of oocysts per female mosquito from 2.5 to 58,6, with a range of 1 to 548. The average number of salivary gland sporozoites per infected mosquito was determined by direct sporozoite counts in the pooled homogenate of the thoraces of all female mosquitoes. In five experiments, it varied from 2.7×10³ to 9.0×10³. The sterile sporozoites, harvested on day 19 or 20 after the infective blood meal, were as infective for rodents as nonsterile ones.Supported in part by Public Health Service research grant AI 18345 from the National Institute of Allergy and Infectious Diseases, by a grant from the Agency of International Development DSPE-5542-G-SS-3042-00, and by a Charles and Johanna Busch award.     

Default Priors for Neural Network Classification

Feedforward neural networks are a popular tool for classification, offering a method for fully flexible modeling. This paper looks at the underlying probability model, so as to understand statistically what is going on in order to facilitate an intelligent choice of prior for a fully Bayesian analysis. The parameters turn out to be difficult or impossible to interpret, and yet a coherent prior requires a quantification of this inherent uncertainty. Several approaches are discussed, including flat priors, Jeffreys priors and reference priors.