Serotonin1A receptor acts during development to establish normal anxiety-like behaviour in the adult

Serotonin is implicated in mood regulation, and drugs acting via the serotonergic system are effective in treating anxiety and depression. Specifically, agonists of the serotonin1A receptor have anxiolytic properties, and knockout mice lacking this receptor show increased anxiety-like behaviour. Here we use a tissue-specific, conditional rescue strategy to show that expression of the serotonin1A receptor primarily in the hippocampus and cortex, but not in the raphe nuclei, is sufficient to rescue the behavioural phenotype of the knockout mice. Furthermore, using the conditional nature of these transgenic mice, we suggest that receptor expression during the early postnatal period, but not in the adult, is necessary for this behavioural rescue. These findings show that postnatal developmental processes help to establish adult anxiety-like behaviour. In addition, the normal role of the serotonin1A receptor during development may be different from its function when this receptor is activated by therapeutic intervention in adulthood.     

Organization and sequence studies of the 17-piece chicken conalbumin gene

The conalbumin gene has been cloned and shown to consist of at least 17 exons approximately 60-200 base pairs long. The DNA sequence upstream from the region coding for the 5' end of the mRNA shows similarities with sequences present in homologous positions in other genes. High and low frequency repetitive sequences are found both upstream from the conalbumin gene and within one intron.     

局部放电脉冲群的实用快速分类技术及应用

对108/s高速采样率获取的超宽带局放脉冲波形-时间序列,使用等效时频法提取了局部放电脉冲群的波形特征参数,并研究了模糊C均值聚类算法的特性及其在局部放电脉冲波形特征向量参数处理中的应用.基于GIS的局部放电试验数据处理表明:由等效时频特征提取和模糊C均值聚类分析组成的局部放电脉冲群快速分类技术可以对多个局部放电源构成的脉冲群进行准确分类.这为研制基于单个脉冲的局部放电超宽带检测与模式识别系统提供了实用的脉冲群快速分类技术.     

履带式移动机器人动力学模型及其反馈控制

履带结构被广泛地应用在移动式机器人的设计当中。为提高其反馈控制模型的精度,该文详细分析了履带式机器人的受力特点,提出了一种适于进行控制器设计的履带机器人模型,并在此基础上,按照反馈线性化的思想,提出了一种履带式机器人稳定路径跟踪控制器的设计方法,同时给出了方法的非奇异条件。该文提出的控制系统模型和路径跟踪方法,为履带式机器人控制系统设计提供了理论依据。     

悬索桥主缆缠绕钢丝的设计

提出了考虑主缆空隙效应的缠绕钢丝预加力计算公式,计算了选择主缆缠绕时机的条件和缠丝的技术要求,算例表明,主缆空隙率和缠绕钢丝的直径与弹性模量是影响缠丝预加力的主要因素。     

模糊逻辑推理在电子病历智能辅助诊断系统中的应用研究

文章将模糊逻辑推理的思想应用于电子病历系统的知识表示和智能推理,在辅助诊断时,根据病人病症进行模糊推理,给出确信度,供医生参考.并用模糊逻辑推理方法在电子病历系统中进行室性早搏的实时自动检测实验,结果表明该方法能帮助医生准确思维,达到有效地降低误诊误治率和减少医疗费用的双重目的.     

野油菜黄单胞菌NK－01生物合成黄原胶的分子遗传学分析

本文采用甲基磺酸乙酯（ＥＭＳ）诱变野油菜黄单胞菌ＮＫ－０１得到多糖合成缺陷的不产粘突变株。经ＳａｌＩ部分酶切得到ＮＫ－０１染色体ＤＮＡ片段，将其连接到广泛寄主载体ｐＲＫ２９３上，在Ｅ．ｃｏｌｉＨＢ１０１中建立完整的ＮＫ－０１基因文库。在协助质粒ｐＲＫ２０１３存在下，不产多糖突变株分别与含基因文库的Ｅ．ｃｏｌｉ菌落群进行三亲接合转移，筛选具有卡那霉素抗性的产粘接合后体。对接合后体进行重组质粒的酶切分析表明，所克隆的ＤＮＡ具有片段重叠。上述重组质粒对另外９株不产多糖突变株互补检测及遗传学分析表明，１３．４ｋｂ的ＤＮＡ片段含有合成黄原胶所必需的基因。