A rapid and accurate 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide colorimetric assay for quantification of bacteriocins with nisin as an example

The objective of this study is to propose a more accurate and faster MTT [3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide] colorimetric assay (MCA) for quantitative measurement of polypeptide bacteriocins in solutions with nisin as an example. After an initial incubation of nisin and indicator bacterium Micrococcus luteus NCIB 8166 in tubes, MTT was added for another incubation period. After that, nisin was quantified by estimating the number of viable bacteria based on measuring the amount of purple formazan produced by cleavage of yellow tetrazolium salt MTT. Then MCA was compared to a standard agar diffusion assay (ADA). The results suggested a high correlation coefficient (r ²=0.975±0.004) between optical density (OD) and the inhibitory effect of nisin on a bacterial strain Micrococcus luteus NCIB 8166 at a range of 0.125~32 IU/ml. The MCA described in this study was very quick. Quantification of nisin took only 7~8 h and the detection limit was at the level of 0.125 IU/ml when compared to 12 IU/ml and 24~28 h for ADA. The MCA provides an accurate and rapid method for quantification of nisin in solutions and is expected to be used for quantification of other antimicrobial substances.     

中晚期高危妊娠引产32例分析及经验总结

目的：分析中晚期妊娠合并症引产病例的特征，适当引产方式的选择。总结引产处理成功的经验。方法：总结32例中晚期妊娠合并内外科疾病选择引产病例，分析各病种分布以及引产方式、引产并发症。结果：32例病例中肝肾疾病占31％，选择利凡诺引产15例，水囊引产12例，药物引产4例。均无内外科并发症发生。结论：做好有妊娠合并症的中晚期引产的术前术中准备工作，选择合适的引产方式是引产成功安全的关键。     

Amniotic membrane transplantation for porous sphere orbital implant exposure

Objective: This study is aimed at describing the clinical outcome of amniotic membrane transplantation for exposure of porous sphere implants. Methods: A retrospective review of consecutive cases of porous sphere orbital implant exposure was carried out. Eight cases were presented between May 2004 and Oct. 2006 (5 males, 3 females; mean age 44.5 years). Six had enucleation and two had evisceration. Exposure occurred in two primary and six secondary. Orbital implant diameter was 22 mm in seven cases and 20 mm in one case. Six patients are with hydroxyapatite and two with high-density porous polyethylene (Medpor) orbital implants. The mean time from implantation to exposure was 1.1 months (range 0.8~2 months). All patients required surgical intervention. Results: The time of follow-up ranged from 3.0 to 28.0 months (mean 16.5 months). Amniotic membrane grafting successfully closed the defect without re-exposure in all of these patients. The grafts were left bare with a mean time to conjunctiva of about 1 month (range 0.8~1.5 months). Conclusion: Exposed porous sphere implants were treated successfully with amniotic membrane graft in all of patients. The graft is easy to harvest. This technique is useful, dose not lead to prolonged socket inflammation and infection, and it is valuable application extensively.     

浅谈利用任务驱动法进行单片机教学与职业核心能力的培养

本文就任务驱动法在单片机教学中的应用与职业核心能力培养进行了阐述，并且以实例的形式得到了很好的验证。     

非有效市场条件下证券交易税实证研究

主要分析在股市存在泡沫的情况下,证券交易税税率提高对采取正反馈投资策略的噪音交易者和理性投资的被动交易者及套利者的影响,在理论不支持征收交易税降低市场波动的宏观调节政策.     

论建立学校安全责任强制保险制度

学校安全事故频繁发生,我国现在学校安全保险法律制度不完善。作者分析了中国目前学校安全责任制度存在的问题,并提出了关于建立学校安全责任绝对强制保险制度的建议。     

葡萄酒月度价格指标的季节ARIMA时间序列分析

以时间序列分析为工具,研究了法国葡萄酒价格月度指标,使用基于周期图的Fisher检验证实该数据指标具有潜在周期,最后发现(1,0,0)×(1,0,0)12模型能较好地拟合数据.在此基础上给出相应的预测.     

Perceptual learning of dot pattern

Dot pattern is a type of pattern defined by specific spatial relationship among some dots. As a compensation for concrete and meaningful visual stimuli, meaningless dot pattern can be used as stimuli in learning tasks. We performed an experiment for exploring the perceptual learning process of dot pattern against random-dot background. Participants were required to learn two types of dot patterns (9-dot and 11-dot). They were assigned to two groups: participants in Group 1 learned 9-dot pattern first and 11-dot pattern later, while those in Group 2 learned 11-dot pattern first and 9-dot pattern later. The results showed that participants could acquire the spatial relationship of dot pattern through perceptual learning in relatively short learning time. In comparison with 9-dot pattern, learning time of 11-dot pattern was slightly longer and its accuracy rate lower, but there was significant positive transfer effect from 11-dot pattern learning to 9-dot learning.     

专业知识在车工实习中的渗透教学

从实习场地、实习教学过程和课后作业等方面对专业知识的渗透教学做了介绍,并通过实例要求学生深刻理解专业知识,熟练掌握车工操作技能,将车工专业知识渗透到实习教学中.     

浅析机械制图教学与创新教育

文章阐述了在机械制图教学中，如何培养学生画图、读图能力与创新能力，以及它们两者间的辩证关系。