Comparing Small‐ and Large‐Scale Models of Multicategory Buying Behavior

Focusing on the interdependence of product categories we analyze multicategory buying decisions of households by a finite mixture of multivariate Tobit‐2 models with two response variables: purchase incidence and expenditure. Mixture components can be interpreted as household segments. Correlations for purchases of different categories turn out to be much more important than correlations among expenditures as well as correlations among purchases and expenditures of different categories. About 18% of all pairwise purchase correlations are significant. We compare the best‐performing large‐scale model with 28 categories to four small‐scale models each with seven categories. In our empirical study the large‐scale model clearly attains a better forecasting performance. The small‐scale models provide several biased correlations and miss about 50% of the significant correlations which the large scale model detects. Copyright © 2013 John Wiley & Sons, Ltd.     

The sculpting of Jupiter's gossamer rings by its shadow

Dust near Jupiter is produced when interplanetary impactors collide energetically with small inner moons, and is organized into a main ring, an inner halo, and two fainter and more distant gossamer rings. Most of these structures are constrained by the orbits of the moons Adrastea, Metis, Amalthea and Thebe, but a faint outward protrusion called the Thebe extension behaves differently and has eluded understanding. Here we report on dust impacts detected during the Galileo spacecraft's traversal of the outer ring region: we find a gap in the rings interior to Thebe's orbit, grains on highly inclined paths, and a strong excess of submicrometre-sized dust just inside Amalthea's orbit. We present detailed modelling that shows that the passage of ring particles through Jupiter's shadow creates the Thebe extension and fully accounts for these Galileo results. Dust grains alternately charge and discharge when traversing shadow boundaries, allowing the planet's powerful magnetic field to excite orbital eccentricities and, when conditions are right, inclinations as well.     

Exact Efficient Handling of Interrupted Illumination in Helical Cone-Beam Computed Tomography with Arbitrary Pitch

We present a theoretically-exact and stable computed tomography(CT) reconstruction algorithm that is capable of handling interrupted illumination and therefore of using all measured data at arbitrary pitch.This algorithm is based on a differentiated backprojection(DBP) on M-lines.First,we discuss the problem of interrupted illumination and how it affects the DBP.Then we show that it is possible to take advantage of some properties of the DBP to compensate for the effects of interrupted illumination in a mat...     

Nanoscale architecture of integrin-based cell adhesions

Cell adhesions to the extracellular matrix (ECM) are necessary for morphogenesis, immunity and wound healing. Focal adhesions are multifunctional organelles that mediate cell-ECM adhesion, force transmission, cytoskeletal regulation and signalling. Focal adhesions consist of a complex network of trans-plasma-membrane integrins and cytoplasmic proteins that form a?<200-nm plaque linking the ECM to the actin cytoskeleton. The complexity of focal adhesion composition and dynamics implicate an intricate molecular machine. However, focal adhesion molecular architecture remains unknown. Here we used three-dimensional super-resolution fluorescence microscopy (interferometric photoactivated localization microscopy) to map nanoscale protein organization in focal adhesions. Our results reveal that integrins and actin are vertically separated by a ～40-nm focal adhesion core region consisting of multiple protein-specific strata: a membrane-apposed integrin signalling layer containing integrin cytoplasmic tails, focal adhesion kinase and paxillin; an intermediate force-transduction layer containing talin and vinculin; and an uppermost actin-regulatory layer containing zyxin, vasodilator-stimulated phosphoprotein and α-actinin. By localizing amino- and carboxy-terminally tagged talins, we reveal talin's polarized orientation, indicative of a role in organizing the focal adhesion strata. The composite multilaminar protein architecture provides a molecular blueprint for understanding focal adhesion functions.     

Evolutionary change from induced to constitutive expression of an indirect plant resistance

Induced plant resistance traits are expressed in response to attack and occur throughout the plant kingdom. Despite their general occurrence, the evolution of such resistances has rarely been investigated. Here we report that extrafloral nectar, a usually inducible trait, is constitutively secreted by Central American Acacia species that are obligately inhabited by ants. Extrafloral nectar is secreted as an indirect resistance, attracting ants that defend plants against herbivores. Leaf damage induces extrafloral nectar secretion in several plant species; among these are various Acacia species and other Fabaceae investigated here. In contrast, Acacia species obligately inhabited by symbiotic ants nourish these ants by secreting extrafloral nectar constitutively at high rates that are not affected by leaf damage. The phylogeny of the genus Acacia and closely related genera indicate that the inducibility of extrafloral nectar is the plesiomorphic or 'original' state, whereas the constitutive extrafloral nectar flow is derived within Acacia. A constitutive resistance trait has evolved from an inducible one, obviously in response to particular functional demands.     

Psoriasis-like skin disease and arthritis caused by inducible epidermal deletion of Jun proteins

Psoriasis is a frequent, inflammatory disease of skin and joints with considerable morbidity. Here we report that in psoriatic lesions, epidermal keratinocytes have decreased expression of JunB, a gene localized in the psoriasis susceptibility region PSORS6. Likewise, inducible epidermal deletion of JunB and its functional companion c-Jun in adult mice leads (within two weeks) to a phenotype resembling the histological and molecular hallmarks of psoriasis, including arthritic lesions. In contrast to the skin phenotype, the development of arthritic lesions requires T and B cells and signalling through tumour necrosis factor receptor 1 (TNFR1). Prior to the disease onset, two chemotactic proteins (S100A8 and S100A9) previously mapped to the psoriasis susceptibility region PSORS4, are strongly induced in mutant keratinocytes in vivo and in vitro. We propose that the abrogation of JunB/activator protein 1 (AP-1) in keratinocytes triggers chemokine/cytokine expression, which recruits neutrophils and macrophages to the epidermis thereby contributing to the phenotypic changes observed in psoriasis. Thus, these data support the hypothesis that epidermal alterations are sufficient to initiate both skin lesions and arthritis in psoriasis.     

Amnionless,essential for mouse gastrulation,is mutated in recessive hereditary megaloblastic anemia

The amnionless gene, Amn, on mouse chromosome 12 encodes a type I transmembrane protein that is expressed in the extraembryonic visceral layer during gastrulation. Mice homozygous with respect to the amn mutation generated by a transgene insertion have no amnion. The embryos are severely compromised, surviving to the tenth day of gestation but seem to lack the mesodermal layers that normally produce the trunk. The Amn protein has one transmembrane domain separating a larger, N-terminal extracellular region and a smaller, C-terminal cytoplasmic region. The extracellular region harbors a cysteine-rich domain resembling those occurring in Chordin, found in Xenopus laevis embryos, and Sog, found in Drosophila melanogaster. As these cysteine-rich domains bind bone morphogenetic proteins (Bmps), it has been speculated that the cysteine-rich domain in Amn also binds Bmps. We show that homozygous mutations affecting exons 1-4 of human AMN lead to selective malabsorption of vitamin B12 (a phenotype associated with megaloblastic anemia 1, MGA1; OMIM 261100; refs. 5,6) in otherwise normal individuals, suggesting that the 5' end of AMN is dispensable for embryonic development but necessary for absorption of vitamin B12. When the 5' end of AMN is truncated by mutations, translation is initiated from alternative downstream start codons.