Drosophila odorant receptors are both ligand-gated and cyclic-nucleotide-activated cation channels

From worm to man, many odorant signals are perceived by the binding of volatile ligands to odorant receptors that belong to the G-protein-coupled receptor (GPCR) family. They couple to heterotrimeric G-proteins, most of which induce cAMP production. This second messenger then activates cyclic-nucleotide-gated ion channels to depolarize the olfactory receptor neuron, thus providing a signal for further neuronal processing. Recent findings, however, have challenged this concept of odorant signal transduction in insects, because their odorant receptors, which lack any sequence similarity to other GPCRs, are composed of conventional odorant receptors (for example, Or22a), dimerized with a ubiquitously expressed chaperone protein, such as Or83b in Drosophila. Or83b has a structure akin to GPCRs, but has an inverted orientation in the plasma membrane. However, G proteins are expressed in insect olfactory receptor neurons, and olfactory perception is modified by mutations affecting the cAMP transduction pathway. Here we show that application of odorants to mammalian cells co-expressing Or22a and Or83b results in non-selective cation currents activated by means of an ionotropic and a metabotropic pathway, and a subsequent increase in the intracellular Ca(2+) concentration. Expression of Or83b alone leads to functional ion channels not directly responding to odorants, but being directly activated by intracellular cAMP or cGMP. Insect odorant receptors thus form ligand-gated channels as well as complexes of odorant-sensing units and cyclic-nucleotide-activated non-selective cation channels. Thereby, they provide rapid and transient as well as sensitive and prolonged odorant signalling.     

A natural polymorphism alters odour and DEET sensitivity in an insect odorant receptor

Blood-feeding insects such as mosquitoes are efficient vectors of human infectious diseases because they are strongly attracted by body heat, carbon dioxide and odours produced by their vertebrate hosts. Insect repellents containing DEET (N,N-diethyl-meta-toluamide) are highly effective, but the mechanism by which this chemical wards off biting insects remains controversial despite decades of investigation. DEET seems to act both at close range as a contact chemorepellent, by affecting insect gustatory receptors, and at long range, by affecting the olfactory system. Two opposing mechanisms for the observed behavioural effects of DEET in the gas phase have been proposed: that DEET interferes with the olfactory system to block host odour recognition and that DEET actively repels insects by activating olfactory neurons that elicit avoidance behaviour. Here we show that DEET functions as a modulator of the odour-gated ion channel formed by the insect odorant receptor complex. The functional insect odorant receptor complex consists of a common co-receptor, ORCO (ref. 15) (formerly called OR83B; ref. 16), and one or more variable odorant receptor subunits that confer odour selectivity. DEET acts on this complex to potentiate or inhibit odour-evoked activity or to inhibit odour-evoked suppression of spontaneous activity. This modulation depends on the specific odorant receptor and the concentration and identity of the odour ligand. We identify a single amino-acid polymorphism in the second transmembrane domain of receptor OR59B in a Drosophila melanogaster strain from Brazil that renders OR59B insensitive to inhibition by the odour ligand and modulation by DEET. Our data indicate that natural variation can modify the sensitivity of an odour-specific insect odorant receptor to odour ligands and DEET. Furthermore, they support the hypothesis that DEET acts as a molecular 'confusant' that scrambles the insect odour code, and provide a compelling explanation for the broad-spectrum efficacy of DEET against multiple insect species.     

Leadership by Activity Theory and Professional Development by Social Construction

Culture and human development come together as one. Contradictions and challenges abound in the transformation of social practices of leadership and management. Globalization, organizational, and professional development bring local realities together. Research must embrace characteristic discourses in order to constitute a modified perspective to current approaches. Research must direct attention to the predominant conceptions of leaders, contexts, and relations. Leaders are discoursed objects capable of individual action, acting rationally in order to structure their contexts. Their ability to relate to others is an individual act and a source to progress. Good discourses displace attitudes, personal properties, and individual acts and highlight relations. One shift of attention goes from transcendental notions of truth, right, and suitability to local–historical constructions of reality. Another shift goes from reality as a singular and independent state to multiple socially constructed realities. In the processes of social construction people create individual worlds where unique relations emerge. Coordinated training and research enable different modes of relating to each other in systemic practice.     

Agrotis segetum雄蛾触角叶神经元的时相编码特性

应用细胞内记录神经放电方法 ,研究Agrotissegetum雄蛾触角叶MGC神经元对性信息素的反应特征及时相编码特点。在此种雄蛾MGC中 ,投射神经元对性信息素的反应呈现一种双相反应模式。 2 6个MGC神经元均可对 1,3Hz频率的每个刺激有明显的双相反应 ,14个神经元可对 5Hz频率的每个刺激引起明显的双相反应 ,4个神经元仅能识别 7Hz频率的每个刺激 ,1个神经元可识别高至 10Hz频率的每个刺激。神经元对性信息素频率刺激的反应能力与神经元自发放电频率、反应时的超极化幅度等都有一定的相关关系     

High-resolution record of Northern Hemisphere climate extending into the last interglacial period

Two deep ice cores from central Greenland, drilled in the 1990s, have played a key role in climate reconstructions of the Northern Hemisphere, but the oldest sections of the cores were disturbed in chronology owing to ice folding near the bedrock. Here we present an undisturbed climate record from a North Greenland ice core, which extends back to 123,000 years before the present, within the last interglacial period. The oxygen isotopes in the ice imply that climate was stable during the last interglacial period, with temperatures 5 degrees C warmer than today. We find unexpectedly large temperature differences between our new record from northern Greenland and the undisturbed sections of the cores from central Greenland, suggesting that the extent of ice in the Northern Hemisphere modulated the latitudinal temperature gradients in Greenland. This record shows a slow decline in temperatures that marked the initiation of the last glacial period. Our record reveals a hitherto unrecognized warm period initiated by an abrupt climate warming about 115,000 years ago, before glacial conditions were fully developed. This event does not appear to have an immediate Antarctic counterpart, suggesting that the climate see-saw between the hemispheres (which dominated the last glacial period) was not operating at this time.     

Lasalleistichus a new genus of Tetrastichinae (Hymenoptera: Eulophidae) from the Neotropical region,including four new species

ABSTRACT

A new genus, Lasalleistichus, is described based on material from the Neotropical region (Brazil, Costa Rica, Peru). This new genus includes four species: L. albiclava, L. albifasciatus, L. fuscicoxa, L. longicaulis, all spp. n. and described here. The group is mainly female based, males are known only for L. albiclava. There is no biological information available for this group.

http://www.zoobank.org/urn:lsid:zoobank.org:pub:82E08F36-E2C4-42FF-AD12-11B49C272AC6     

Praxis Relevance in Science

Science is praxis relevant to the extent that it guides goal-directed action by telling us how to act in order to achieve the goals. Investigations aiming at high praxis relevance are performed in various disciplines under names such as clinical trials, evaluation research, intervention research and social experiments. In this contribution, the notion of (direct) praxis relevance is delineated, and it is distinguished from related properties of science such as those of being applied and being practically useful in a wider sense. Recommendations for the achievement of praxis relevance are offered in the form of five principles: the prerogative of direct experiments, minimized theory-induced uncertainty, multiple approximations, causal chain decomposition and successive improvements.