全文获取类型
收费全文 | 135篇 |
免费 | 1篇 |
专业分类
系统科学 | 1篇 |
理论与方法论 | 10篇 |
现状及发展 | 16篇 |
研究方法 | 18篇 |
综合类 | 77篇 |
自然研究 | 14篇 |
出版年
2017年 | 2篇 |
2015年 | 1篇 |
2014年 | 1篇 |
2013年 | 3篇 |
2012年 | 3篇 |
2011年 | 25篇 |
2010年 | 1篇 |
2008年 | 11篇 |
2007年 | 7篇 |
2006年 | 6篇 |
2005年 | 7篇 |
2004年 | 8篇 |
2003年 | 6篇 |
2002年 | 3篇 |
2001年 | 5篇 |
2000年 | 3篇 |
1999年 | 1篇 |
1998年 | 1篇 |
1996年 | 1篇 |
1995年 | 2篇 |
1992年 | 1篇 |
1991年 | 2篇 |
1990年 | 4篇 |
1989年 | 3篇 |
1988年 | 1篇 |
1987年 | 1篇 |
1985年 | 1篇 |
1984年 | 1篇 |
1983年 | 3篇 |
1982年 | 1篇 |
1980年 | 1篇 |
1979年 | 2篇 |
1978年 | 2篇 |
1977年 | 2篇 |
1976年 | 1篇 |
1974年 | 1篇 |
1972年 | 1篇 |
1971年 | 1篇 |
1970年 | 1篇 |
1969年 | 1篇 |
1968年 | 1篇 |
1967年 | 1篇 |
1966年 | 2篇 |
1965年 | 2篇 |
1961年 | 1篇 |
1957年 | 1篇 |
排序方式: 共有136条查询结果,搜索用时 630 毫秒
81.
E Hough L K Hansen B Birknes K Jynge S Hansen A Hordvik C Little E Dodson Z Derewenda 《Nature》1989,338(6213):357-360
Both the phosphatidylinositol-hydrolysing and the phosphatidylcholine-hydrolysing phospholipases C have been implicated in the generation of second messengers in mammalian cells. The phosphatidylcholine-hydrolysing phospholipase C (PLC) from Bacillus cereus, a monomeric protein containing 245 amino-acid residues, is similar to some of the corresponding mammalian proteins. This, together with the fact that the bacterial enzyme can mimic the action of mammalian PLC in causing, for example, enhanced prostaglandin biosynthesis, suggests that B. cereus PLC can be used as a model for the hitherto poorly characterized mammalian PLCs. We report here the three-dimensional structure of B. cereus PLC at 1.5 A resolution. The enzyme is an all-helix protein belonging to a novel structural class and contains, at least in the crystalline state, three Zn2+ in the active site. We also present preliminary results from a study at 1.9 A resolution of the complex between PLC and inorganic phosphate (Pi) which indicate that the substrate binds directly to the metal ions. 相似文献
82.
Immunopathology of schistosomiasis in athymic mice. 总被引:3,自引:0,他引:3
83.
The stability of the salt content in Salicornia pacifica Standl. var. utahensis (Tidestrom) Munz in relation to environmental changes was investigated. Salicornia pacifica communities have a characteristic soil pH of 7.5 to 8.0 ± 0.2 and a constant subsurface soil moisture level of 25 to 35 percent. The ion content in the tissue of S. pacifica remained constant despite increased moisture stress throughout the growing season. The concentrations of the salts were significantly higher in the surface soil layers than in the subsurface layers around the roots. Normal metabolic processes in the tissues of S. pacifica appear to occur even though some fluctuations in the ionic balance and concentration of ions in the plant occur. 相似文献
84.
Burrowing Owls ( Athene cunicularia ) were studied in a prairie dog town of southwestern South Dakota. Pellets regurgitated by Burrowing Owls contained a wide variety of prey remains. Insects, spiders, small mammals, and vegetation were the most frequent items identified in the pellets. Mammals were consumed most frequently during spring and early summer. Insects were consumed in large numbers during the entire period of this study, but they became more frequent in owl pellets during late summer and fall in association with a decline of mammal remains. Some prey items observed around owl nest sites were not found in the pellets examined. Possible secondary poisoning of some prey of Burrowing Owls has not produced any change in owl food habits, based on other studies reported in the literature. 相似文献
85.
Caitlin Collin Frank Hauser Ernesto Gonzalez de Valdivia Shizhong Li Julia Reisenberger Eva M. M. Carlsen Zaid Khan Niels Ø. Hansen Florian Puhm Leif Søndergaard Justyna Niemiec Magdalena Heninger Guilin R. Ren Cornelis J. P. Grimmelikhuijzen 《Cellular and molecular life sciences : CMLS》2013,70(21):4197-4197
86.
Strained silicon as a new electro-optic material 总被引:1,自引:0,他引:1
Jacobsen RS Andersen KN Borel PI Fage-Pedersen J Frandsen LH Hansen O Kristensen M Lavrinenko AV Moulin G Ou H Peucheret C Zsigri B Bjarklev A 《Nature》2006,441(7090):199-202
For decades, silicon has been the material of choice for mass fabrication of electronics. This is in contrast to photonics, where passive optical components in silicon have only recently been realized. The slow progress within silicon optoelectronics, where electronic and optical functionalities can be integrated into monolithic components based on the versatile silicon platform, is due to the limited active optical properties of silicon. Recently, however, a continuous-wave Raman silicon laser was demonstrated; if an effective modulator could also be realized in silicon, data processing and transmission could potentially be performed by all-silicon electronic and optical components. Here we have discovered that a significant linear electro-optic effect is induced in silicon by breaking the crystal symmetry. The symmetry is broken by depositing a straining layer on top of a silicon waveguide, and the induced nonlinear coefficient, chi(2) approximately 15 pm V(-1), makes it possible to realize a silicon electro-optic modulator. The strain-induced linear electro-optic effect may be used to remove a bottleneck in modern computers by replacing the electronic bus with a much faster optical alternative. 相似文献
87.
88.
Theologis A Ecker JR Palm CJ Federspiel NA Kaul S White O Alonso J Altafi H Araujo R Bowman CL Brooks SY Buehler E Chan A Chao Q Chen H Cheuk RF Chin CW Chung MK Conn L Conway AB Conway AR Creasy TH Dewar K Dunn P Etgu P Feldblyum TV Feng J Fong B Fujii CY Gill JE Goldsmith AD Haas B Hansen NF Hughes B Huizar L Hunter JL Jenkins J Johnson-Hopson C Khan S Khaykin E Kim CJ Koo HL Kremenetskaia I Kurtz DB Kwan A Lam B Langin-Hooper S Lee A Lee JM Lenz CA Li JH Li Y Lin X Liu SX Liu ZA Luros JS 《Nature》2000,408(6814):816-820
The genome of the flowering plant Arabidopsis thaliana has five chromosomes. Here we report the sequence of the largest, chromosome 1, in two contigs of around 14.2 and 14.6 megabases. The contigs extend from the telomeres to the centromeric borders, regions rich in transposons, retrotransposons and repetitive elements such as the 180-base-pair repeat. The chromosome represents 25% of the genome and contains about 6,850 open reading frames, 236 transfer RNAs (tRNAs) and 12 small nuclear RNAs. There are two clusters of tRNA genes at different places on the chromosome. One consists of 27 tRNA(Pro) genes and the other contains 27 tandem repeats of tRNA(Tyr)-tRNA(Tyr)-tRNA(Ser) genes. Chromosome 1 contains about 300 gene families with clustered duplications. There are also many repeat elements, representing 8% of the sequence. 相似文献
89.
90.
Atomic-scale imaging of carbon nanofibre growth 总被引:1,自引:0,他引:1
Helveg S López-Cartes C Sehested J Hansen PL Clausen BS Rostrup-Nielsen JR Abild-Pedersen F Nørskov JK 《Nature》2004,427(6973):426-429
The synthesis of carbon nanotubes with predefined structure and functionality plays a central role in the field of nanotechnology, whereas the inhibition of carbon growth is needed to prevent a breakdown of industrial catalysts for hydrogen and synthesis gas production. The growth of carbon nanotubes and nanofibres has therefore been widely studied. Recent advances in in situ techniques now open up the possibility of studying gas-solid interactions at the atomic level. Here we present time-resolved, high-resolution in situ transmission electron microscope observations of the formation of carbon nanofibres from methane decomposition over supported nickel nanocrystals. Carbon nanofibres are observed to develop through a reaction-induced reshaping of the nickel nanocrystals. Specifically, the nucleation and growth of graphene layers are found to be assisted by a dynamic formation and restructuring of mono-atomic step edges at the nickel surface. Density-functional theory calculations indicate that the observations are consistent with a growth mechanism involving surface diffusion of carbon and nickel atoms. The finding that metallic step edges act as spatiotemporal dynamic growth sites may be important for understanding other types of catalytic reactions and nanomaterial syntheses. 相似文献