PPAR γ partial agonist, KR-62776, inhibits adipocyte differentiation via activation of ERK

Indenone KR-62776 acts as an agonist of PPARγ without inducing obesity in animal models and cells. X-ray crystallography reveals that the indenone occupies the binding pocket in a different manner than rosiglitazone. 2-Dimensional gel-electrophoresis showed that the expression of 42 proteins was altered more than 2.0-fold between KR-62776- or rosiglitazone-treated adipocyte cells and control cells. Rosiglitazone down-regulated the expression of ERK1/2 and suppressed the phosphorylation of ERK1/2 in these cells. However, the expression of ERK1/2 was up-regulated in KR-62776-treated cells. Phosphorylated ERK1/2, activated by indenone, affects the localization of PPARγ, suggesting a mechanism for indenone-inhibition of adipogenesis in 3T3-L1 preadipocyte cells. The preadipocyte cells are treated with ERK1/2 inhibitor PD98059, a large amount of the cells are converted to adipocyte cells. These results support the conclusion that the localization of PPARγ is one of the key factors explaining the biological responses of the ligands. Received 04 March 2009; received after revision 13 March 2009; accepted 17 March 2009     

一种新的蠕虫检测和控制方法

在分析网络蠕虫连接请求和网络正常连接请求差异的基础上,提出一种新的蠕虫检测和控制方法.该方法针对网络蠕虫攻击特定端口以及攻击地址发散的特性,采用基于端口的多工作集区分网络蠕虫连接请求和网络正常连接请求,在蠕虫控制中使用多延迟队列处理可疑连接请求,避免了不同端口流量之间的相互影响;针对网络正常连接请求的暂时突发特征,利用令牌桶控制多延迟队列的输出,缩短了正常连接请求在延迟队列中的停留时间.测试表明,在主机感染了蠕虫后,新方法将误报率从85%降低到12%,对正常连接请求的平均延迟时间从95.4s降低到5.6s.     

二甲醚HCCI发动机微量排放物的研究

根据优化气相色谱仪工作条件,对二甲醚均质充量压燃(HCCI)发动机排气中的甲醛和甲酸甲酯进行了检测.通过二甲醚简化反应机理与Fluent的耦合联算,以及结合Woschni传热模型和DME详细反应机理的Chemkin反应动力学数值模拟,对二甲醚HCCI发动机的燃烧过程及其微量排放物(甲醛、甲酸和甲酸甲酯)进行了预测分析.结果表明:在一定条件下,甲醛、甲酸和甲酸甲酯存在于排气中,其排放量均随过量空气系数的减小而减小,发动机转速、进气温度和压力对其影响也很大.     

仿生六足机器人步态规划策略实验研究

采用日本弓背蚁进行常规步态实验学研究和仿生学探索,通过对高速数字摄像机采样结果的判断和分析,发现了蚂蚁后足的滑行过程并定义为“滑动相“.此外,还针对蚂蚁直线前进步态给出了合理的步态关系表达式,通过与周期规则步态的对比证明了该式的现实可行性,为仿生六足机器人进行周期不规则步态的规划奠定了理论基础.所采用的实验学研究和仿生学探索相结合的方法能有效提高仿生机器人的技术水平.     

BRD7 regulates the insulin-signaling pathway by increasing phosphorylation of GSK3β

Reduced hepatic expression levels of bromodomain-containing protein 7 (BRD7) have been suggested to play a role in the development of glucose intolerance in obesity. However, the molecular mechanism by which BRD7 regulates glucose metabolism has remained unclear. Here, we show that BRD7 increases phosphorylation of glycogen synthase kinase 3β (GSK3β) in response to activation of the insulin receptor-signaling pathway shortly after insulin stimulation and the nutrient-sensing pathway after feeding. BRD7 mediates phosphorylation of GSK3β at the Serine 9 residue and this effect on GSK3β occurs even in the absence of AKT activity. Using both in vitro and in vivo models, we further demonstrate that BRD7 mediates phosphorylation of ribosomal protein S6 kinase (S6K) and leads to increased phosphorylation of the eukaryotic translation initiation factor 4E-binding protein 1 (4E-BP1) and, therefore, relieves its inhibition of the eukaryotic translation initiation factor 4E (eIF4E). However, the increase in phosphorylation of 4E-BP1 with BRD7 overexpression is blunted in the absence of AKT activity. In addition, using liver-specific BRD7 knockout (LBKO) mice, we show that BRD7 is required for mTORC1 activity on its downstream molecules. These findings show a novel basis for understanding the molecular dynamics of glucose metabolism and suggest the unique function of BRD7 in the regulation of glucose homeostasis.     

一种新的模糊自校正控制学习算法

提出一种基于模糊竞争学习的模糊自校正控制方法.通过基于模糊竞争学习确定一种在线模糊辨识算法,在采用此模糊辨识方法对对象进行在线估计的基础上,用调节器实现参数的自动整定.为了验证所提方法的有效性,给出了非线性系统的控制结果.     

柔臂弯曲和挠转振动的非线性应力反馈控制

用数值仿真的方法讨论了一种用新型的应力及其微分的非线性反馈律来镇定柔性臂的弯曲和扭转振动的问题。该法使用跟踪－微分器取得柔性臂根部应力信号和微分信号,并与根部应力信号以及端咪的挠率信号一起组成非线性控制输入来控制柔性臂的弯曲和扭转振动。其镇定的仿真实验效果很好。     

滞后中立型线性离散系统的滞后无关稳定性判据

给出了几个滞后中立型线性离散系统稳定性的充分条件，由于这些条件与滞后无关且形成简单，对于初步判定其稳定性提供了有用的工具。     

金属有机化合物化学气相淀积含钛硬质涂层的性能研究

在用文题淀积方法制备出氮化钛、碳氮化钛和碳氮氧化钛硬质涂层的基础上,用表面分析手段分别进行了成分、结构分析、形貌观察和硬度测定。结果表明:以二乙胺基钛为原料可分别在773K和973K淀积氮化钛和碳氮化钛涂层;用钛酸异丙酯和钛酸丁酯可分别在973K和1073K获得碳氮氧化钛涂层。所得的涂层表面光洁度高、与基体附着性好、硬度满足实用要求。相比于普通化学气相淀积方法,本法在制备硬质涂层上有两大优点:(1)淀积温度降低,扩大了基体的选用范围;(2)固溶体涂层的获得扩大了涂层的适用范围。