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21.
22.
构建T7噬菌体展示禽流感病毒抗原变异性基因片段文库. 首先, 从Gene Bank中查找筛选禽流感病毒抗原变异性基因, 将其截短、 修饰、 简并后得到禽流感病毒抗原变异性基因微阵列. 其次, 将合成的禽流感病毒抗原变异性基因片段文库扩增、 酶切, 链接到双酶切后的T7噬菌体载体基因上, 构成重组噬菌体DNA. 最后, 重组噬菌体DNA经体外包装和扩增, 得到T7噬菌体展示文库, 并进行T7噬菌体展示文库滴度、 重组率和免疫活性测定. 实验结果表明, 从Gene Bank中查找、 筛选、 剪切和修饰共获得96 258条序列构建T7噬菌体展示文库, 原始文库滴度为3.6×107个菌落/mL, 重组率大于90%. 用禽流感病毒H5N1抗体进行捕获, 经聚合酶链式反应(PCR)鉴定, 得到理想目的条带, 证明噬菌体表面展示蛋白具有抗原活性, 可用于禽流感病毒感染患者的快速检测及抗原表位筛选. 相似文献
23.
Static extracting method of software intended behavior based on API functions invoking 总被引:1,自引:0,他引:1
The method of extracting and describing the intended behavior of software precisely has become one of the key points in the fields of software behavior's dynamic and trusted authentication. In this paper, the author proposes a specified measure of extracting SIBDS (software intended behaviors describing sets) statically from the binary executable using the software's API functions invoking, and also introduces the definition of the structure used to store the SIBDS in detail. Experimental results demonstrate that the extracting method and the storage structure definition offers three strong properties: (i) it can describe the software's intended behavior accurately; (ii) it demands a small storage expense; (iii) it provides strong capability to defend against mimicry attack. 相似文献
24.
XIE Xianjian WEI Fangqiang BAI Jingwen LAI Ting 《武汉大学学报:自然科学英文版》2007,12(4):729-736
In this experiment, using the methods of drought-stress in the pot and PEG simulative drought-stress, three native rocky-slope grasses Pogonatherum panideum(Lam.) Hack, Erioophorum comosum nees and Cynodon dactylon cultivated in the pots were selected as materials to study their drought-resistance by analyzing the indexes such as the leaf area index, the drying roots accumulation, the holding water ability of leaf, the relative conductance of leaf, the soluble protein, chlorophyll. At the same time, by comparing with membership function value of each index, the order of each grass drought-resistance was decided. The results showed that the drought-resistance of Erioophorum comosum nees was stronger than Cynodon dactylon and drought-resistance of Cynodon dactylon was stronger than Pogonatherum panideum (Lam.) Hack. The purpose of the experimental results was to find theoretical foundations for selecting and cultivating native protecting-slope vegetations to adapt to rocky slopes. 相似文献
25.
WANG Xibo ZHOU Benhai YU Gei LI Qian 《武汉大学学报:自然科学英文版》2007,12(5):946-950
μC/OS-Ⅱ is an open source real-time kernel adopting priority preemptive schedule strategy. Aiming at the problem of μC/OS-Ⅱ failing to support homology priority tasks scheduling, an approach for solution is proposed. The basic idea is adding round-robin scheduling strategy in its original scheduler in order to schedule homology priority tasks through time slice roundrobin. Implementation approach is given in detail. Firstly, the Task Control Block (TCB) is extended. And then, a new priority index table is created, in which each index pointer points to a set of homology priority tasks. Eventually, on the basis of reconstructing μC/OS-Ⅱ real-time kernel, task scheduling module is rewritten. Otherwise, schedulability of homology task supported by modified kernel had been analyzed, and deadline formula of created homology tasks is given. By theoretical analysis and experiment verification, the modified kernel can support homology priority tasks scheduling, meanwhile, it also remains preemptive property of original μC/OS-Ⅱ. 相似文献
26.
LUO HaiYing WANG YunFang KONG Wei PEI XueTao 《科学通报(英文版)》2007,52(18):2449-2456
Today, liver transplantation (LT) is the only established treatment for end-stage liver diseases. The de- velopment of LT, including OLT, cadaveric LT, split LT, living donor LT (LDLT), brings hopes to patients with these diseases. However, increasing donor shortage, rejection and life-long immunosuppression with its side effects are the major limitations of this therapy strategy. Bone marrow-derived stem cells (BMDSCs) are capable of differentiating into hepatocyte-like cells and contribute to liver injury repair. The microenvironment of liver injury caused by rejection, ischemia/reperfusion, loss of liver mass, recurrence of HCV and "small-for-size syndrome" after LT can attract a variety of bone marrow-derived stem cell population to the peripheral circulation and then migration to the injury liver to promote the hepatic function restoration. Additionally, BMDSCs can also take part in the functional regeneration of living donor liver after LDLT. This participation in liver regeneration may be associated to the interac- tion between SDF-1and its receptor CXCR4, involving HGF, IL-8, MMP9, and VEGF/VEGFR-2. BMDSC with its bio-characteristics could maintain the allograft tolerance from different angles and in different ways. In conclusion, BMDSCs transplantation, as a new assistant therapeutic method for LT, will ex- pand the space of LT, and provide more survival opportunities for the patients suffering liver diseases in the future. 相似文献
27.
28.
安徽省豹猫的分布和数量 总被引:3,自引:0,他引:3
应用皮张收购资料的统计和访问调查、野外实地考察等方法,研究了豹猫在安徽省的分布、数量及历史变化。 相似文献
29.
30.
The exponential Radon transform, a generalization of the Radon transform, is defined and studied as a mapping of function
spaces. It is represented in terms of Fourier transform of its domain and range, and this leads to the harmonic decomposition
reconstruction. The results are similar results of Tretiak and Metz.
Foundation item: Supported by the National Natural Science Foundation of China (No. 19971064), Key Project of Science and
Technology of Hubei Province Education Committee.
Biography: Wang Jin-ping (1963-), male, Ph.D. candidate, research direction: numberical solution of singular integral equation
and integral transformation etc. 相似文献