VEGF regulates haematopoietic stem cell survival by an internal autocrine loop mechanism

Vascular endothelial growth factor (VEGF) is a principal regulator of blood vessel formation and haematopoiesis, but the mechanisms by which VEGF differentially regulates these processes have been elusive. Here we describe a regulatory loop by which VEGF controls survival of haematopoietic stem cells (HSCs). We observed a reduction in survival, colony formation and in vivo repopulation rates of HSCs after ablation of the VEGF gene in mice. Intracellularly acting small-molecule inhibitors of VEGF receptor (VEGFR) tyrosine kinase dramatically reduced colony formation of HSCs, thus mimicking deletion of the VEGF gene. However, blocking VEGF by administering a soluble VEGFR-1, which acts extracellularly, induced only minor effects. These findings support the involvement in HSC survival of a VEGF-dependent internal autocrine loop mechanism (that is, the mechanism is resistant to inhibitors that fail to penetrate the intracellular compartment). Not only ligands selective for VEGF and VEGFR-2 but also VEGFR-1 agonists rescued survival and repopulation of VEGF-deficient HSCs, revealing a function for VEGFR-1 signalling during haematopoiesis.     

关于老子思想现代意义的哲学反思

老子的思想可,包含了四方面的精华,对现今人类仍具广泛的现实意义：一是“无为而无不为”的政治艺术;二是“绝学无忧”,对科技与文化异化状态的超越;三是自然而然的生态伦理;四是不为物役,重身轻名的人生哲思。     

中日铁路项目国民经济评价方法比较与分析

以日本《铁路费用效益分析手册 (99版 )》和中国《铁路建设项目经济评价办法 (第 2版 )》为参照 ,通过对比分析中日铁路项目国民经济评价方法的差异 ,提出日本铁路费用效益分析方法中的可借鉴之处 :借鉴日本评价方法分 3类界定受益群体的思路 ,解决我国现行方法存在的效益重复计算问题 ;以福利经济学和“有无对比法”作为项目评价的基本指导原则 ,解决我国由于评价原则模糊导致的同一项目不同评价结论的问题等     

Induced recovery of defective membrane expression of a CC chemokine receptor 5 mutant by phytohemagglutinin

CC chemokine receptor 5 (CCR5) is a member of the G-protein-coupled receptor superfamily. It plays an important role in macrophage tropic human immunodeficiency virus-1 entry and in some inflammatory reactions. CCR5-893(–) is a single-nucleotide deletion that results in complete truncation of the C tail of the gene product. We detected CCR5-893(–) in a sample of patients infected with non-tuberculosis mycobacteria and found that it was maintained heterozygously with a frequency of 2%. There is no association between this mutation and any immunodeficiency. Membrane expression of CCR5-893(–) was substantially reduced compared to the wild type, but this defective surface presentation recovered greatly recovered in the presence of 2 mg l-1 phytohemagglutinin (PHA). However, PHA inducement did not affect the total intracellular expression of CCR5-893(–) or wild-type CCR5. Thus we suggest there exist some PHA-induced factor(s) that could mediate the presentation of truncated CCR5.Received 23 July 2003; accepted 18 August 2003     

高校图书馆“学科馆员”的角色定位

阐述了现代信息社会高校图书馆建立“学科馆员”制度的必然性,从学历水平、能力水平等方面探讨了“学科馆员”的角色定位与工作职责定位。     

抓"软"管理 提升核心竞争力

从更新观念,推动改革和创新;营造企业内外发展的良好氛围;构筑企业文化,提升企业核心竞争力3个方面阐述了抓“软”管理,对提升企业核心竞争力的重要作用。     

Research on data combination for Phased-array Ground Penetrating Radar

0　IntroductionGroundPenetratingRadar (GPR)isanewandefficientex ploringmeanstodetecttheobjectinshallowsubsurface.Comparedwithotherdetectingmethods,GPRhasmanyadvan tages,suchasquickness,highresolution ,convenientoperationetc.SoGPRisappliedtonationaldefenseandnationaleconomy .IndevelopmentofGPRtechnology ,therecomeforthcontinuous waveradar,FMcontinuous waveradar,andshockpulseradar.Amongoftheseradars,shockpulseradarsendsgreatamplitudeimpulsewithperiodinns,receivingequivalentsamplingtoreali…     

A CORBA-Based Multidatabase System——Panorama Project

0　ＩｎｔｒｏｄｕｃｔｉｏｎＴｈｅｎｅｅｄｆｏｒｉｎｔｅｇｒａｔｉｏｎｏｆｄｉｆｆｅｒｅｎｔｄａｔａｂａｓｅｍａｎａｇｅｍｅｎｔｓｙｓｔｅｍｓ (ＤＢＭＳｓ) ,ｍａｋｉｎｇｄｉｆｆｅｒｅｎｃｅｓｂｅｔｗｅｅｎｔｈｅｓｅｓｙｓｔｅｍｓｉｎｖｉｓｉｂｌｅａｎｄｐｒｏｖｉｄｉｎｇｕｓｅｒｓｗｉｔｈａｕｎｉｆｏｒｍａｃｃｅｓｓｔｏａｌｌｔｈｅｄａｔａｂａｓｅｓｈａｓｇｒｏｗｎｒａｐｉｄｌｙｄｕｒｉｎｇｔｈｅｌａｓｔｄｅｃａｄｅ .Ｈｏｗｅｖｅｒ,ｄａｔａｂａｓｅａｕｔｏｎｏｍｙａｎｄｈｅｔｅｒｏｇｅｎｅｉｔｙ…     

竞技体育社会评价指标体系构建研究

为实现竞技体育的可持续发展,有必要对竞技体育进行社会评价、根据“资源配置--运行--效益”模式,科学构建竞技体育社会评价的指标体系,有利于竞技体育的良性运行,有利于奥运战略的完成和竞技体育的可持续发展、     

商业银行构建"金融超市"路径初探

文章阐述了商业银行构建“金融超市”的成因、必要性,提出通过商业银行经营实体的微观金融创新和金融监管部门的金融制度创新互动来构建“金融超市”。