N-terminal of L protein of vesicular stomatitis virus contains a new signal sequence

The L protein (241kD) of vesicular stomatitis virus (VSV) is the most important snbnnit of the replication complex. The existence of specific localization signal in the L protein was investigated by making recombinant constructs expressing truncated mutants of the L protein fused to green fluorescent protein (GFP) in transient transfection assays. The chimeric genes encoding varied N-terminal of L and GFP gene were put under the control of T7 promoter or CMV promoter. The fusion proteins were transiently expressed in BHK-21, COS-7, CHO or Hep G2 cells. When more than 120 residues were deleted or only 96 residues were kept on the N-terminal, the fusion proteins were shown to be distributed throughout the cells, cytoplasm and nucleus under the confocal microscope. However, other chimeric proteins with 120 or more amino acids were dotted and distributed in the perinuclear regions. And the fusion protein with 96—120 aa has the similar distribution. A thirteen-residue peptide QGYSFLHEVDKEA (108—120) was identified as localization signal, whose function would be absolutely distributed with the deficiency of D or V. Our results show that there is an independent localizing signal in N-terminal domain of L protein of VSV and this functional signal is conserved in different cell lines.     

Contributions of different land cover types in Otindag Sandy Land and Bashang area of Hebei Province to the material source of sand stormy weather in Beijing

Different land cover types in Otindag Sandy Land and Bashang area of Hebei Province are linked to a material source of sand stormy weather in Beijing based on results of field vegetation and soil survey and laboratory works. Results of grain size analysis show that dust release potential in per unit area of moving sandy land is small,while lowland meadow and meadow steppe in stony hills have high potential of releasing dusts in per unit area during sand stormy weather occurrence. Further considering the effects of vegetation, it is inferred that the moving sandy land served as a material source of past dust storm and the possibility of dust release in per unit area is low in current time.Typical steppe in stony hills is undergoing desertification and its dust release possibility in per unit area is high. Farmland has strong potential of release dusts when they are ploughed in spring, but a large amount of therophytes grow and thus prevent dusts from release when cultivation was terminated.Potentials of dust release in per unit area in fixed sand dunes,stony mountain meadow steppe and low land meadow are lowdue to high cover of perennials. Sand dune reactivation and desiccation of lakes and lowlands under estimated future climatic change will make them serve as a future material source of sand stormy weather.     

李群G在流形M上左作用的相关性质

本通过李群G在流形M上左作用，构造了M上单参数可微变换群，证明了其诱导向量场与李代数g之间存在同态映射，且诱导向量场是—李代数。     

简析微分几何研究中的思想和方法

本简要分析了微分几何研究中处理局部与整体关系的思想以及通过附加结构利用分析、代数工具进行研究的方法。     

一种素数域上的非超奇椭圆曲线构造方案

对基域为素数域的非超奇椭圆曲线进行了研究，给出了构造素域上具有特定素数阶的非超奇椭圆曲线的一种算法，分析了算法时间复杂度．     

邛崃波尔山羊场牧业生态系统能流物流的研究

运用生态学的系统分析方法，以邓崃波尔山羊场牧业生态系统为研究对象，对其能流、物流以及能量和物质的转化效率和经济效益进行了研究．结果表明，该牧业生态系统以畜产品及畜体输出的能量和粗蛋白质分别占相应总输出的31．07％和31．77％，家畜能量和粗蛋白质的转化效率分别为16．560／o和17．81％，产值盈利率为36．42％，其中羊高达52．26％．该系统是一个值得推广的牧业生态系统．     

到酉群实形式的调和映射

研究从单连通区域Ω R2 ∪ {∞ }到酉群U(N)的某一类实形式G(包括正交群和辛群 )的调和映射 ,引入了G uniton和扩张G uniton的概念 ,并通过两次dressing作用和奇异Darboux变换给出了G uniton及其旗因子的纯代数构造方法 .证明了任意具有有限uniton数的调和映射 φ :Ω →G可因子分解为有限个G uniton的乘积     

三种曝气器在染料化工废水中充氧能力的比较

以某染料化工废水为试验对象 ,选择了三种不同规格型号的微孔曝气器 ,分析比较了其在不同工况条件下的供氧能力、氧利用率和需气量 ,并根据试验结果对该化工废水处理站的供氧系统作了综合评估 .评估结果对废水处理中曝气器的合理选用和供氧系统的正确评估具有参考意义     

反应热喷涂Al2O3.TiO2陶瓷涂层性能的研究

利用自蔓延高温合成原理(SHS)结合传统燃气喷涂技术,研究了反应热喷涂工艺制备的Al2O3.TiO2基金属陶瓷涂层的性能。结果表明,铝热反应热喷涂技术能够促进涂层性能的提高。涂层平均结合强度达17.50MPa以上,平均孔隙率降低到5.50%以下。     

谈谈多媒体课件教学的三大误区

在肯定多媒体课件教学在当今现代教育技术中的作用和优势的同时，剖析了现阶段多媒体课件在教学中的应用现状及存在的问题，探讨了如何正确应用多媒体课件教学这一先进教学手段以获得良好的教学效果。