全文获取类型
收费全文 | 17070篇 |
免费 | 39篇 |
国内免费 | 75篇 |
专业分类
系统科学 | 57篇 |
丛书文集 | 173篇 |
教育与普及 | 68篇 |
理论与方法论 | 55篇 |
现状及发展 | 7669篇 |
研究方法 | 683篇 |
综合类 | 8316篇 |
自然研究 | 163篇 |
出版年
2012年 | 203篇 |
2011年 | 351篇 |
2009年 | 100篇 |
2008年 | 266篇 |
2007年 | 296篇 |
2006年 | 271篇 |
2005年 | 289篇 |
2004年 | 333篇 |
2003年 | 297篇 |
2002年 | 282篇 |
2001年 | 475篇 |
2000年 | 500篇 |
1999年 | 342篇 |
1994年 | 305篇 |
1992年 | 275篇 |
1991年 | 226篇 |
1990年 | 288篇 |
1989年 | 261篇 |
1988年 | 263篇 |
1987年 | 283篇 |
1986年 | 294篇 |
1985年 | 340篇 |
1984年 | 236篇 |
1983年 | 259篇 |
1982年 | 212篇 |
1981年 | 206篇 |
1980年 | 245篇 |
1979年 | 509篇 |
1978年 | 434篇 |
1977年 | 394篇 |
1976年 | 318篇 |
1975年 | 392篇 |
1974年 | 476篇 |
1973年 | 422篇 |
1972年 | 433篇 |
1971年 | 564篇 |
1970年 | 612篇 |
1969年 | 534篇 |
1968年 | 537篇 |
1967年 | 463篇 |
1966年 | 411篇 |
1965年 | 294篇 |
1964年 | 130篇 |
1959年 | 166篇 |
1958年 | 322篇 |
1957年 | 253篇 |
1956年 | 202篇 |
1955年 | 200篇 |
1954年 | 193篇 |
1948年 | 167篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
981.
Functional haploinsufficiency of the human homeobox gene MSX2 causes defects in skull ossification 总被引:10,自引:0,他引:10
Wilkie AO Tang Z Elanko N Walsh S Twigg SR Hurst JA Wall SA Chrzanowska KH Maxson RE 《Nature genetics》2000,24(4):387-390
The genetic analysis of congenital skull malformations provides insight into normal mechanisms of calvarial osteogenesis. Enlarged parietal foramina (PFM) are oval defects of the parietal bones caused by deficient ossification around the parietal notch, which is normally obliterated during the fifth fetal month. PFM are usually asymptomatic, but may be associated with headache, scalp defects and structural or vascular malformations of the brain. Inheritance is frequently autosomal dominant, but no causative mutations have been identified in non-syndromic cases. We describe here heterozygous mutations of the homeobox gene MSX2 (located on 5q34-q35) in three unrelated families with PFM. One is a deletion of approximately 206 kb including the entire gene and the others are intragenic mutations of the DNA-binding homeodomain (RK159-160del and R172H) that predict disruption of critical intramolecular and DNA contacts. Mouse Msx2 protein with either of the homeodomain mutations exhibited more than 85% reduction in binding to an optimal Msx2 DNA-binding site. Our findings contrast with the only described MSX2 homeodomain mutation (P148H), associated with craniosynostosis, that binds with enhanced affinity to the same target. This demonstrates that MSX2 dosage is critical for human skull development and suggests that PFM and craniosynostosis result, respectively, from loss and gain of activity in an MSX2-mediated pathway of calvarial osteogenic differentiation. 相似文献
982.
983.
In model organisms, chemical mutagenesis provides a powerful alternative to natural, polygenic variation (for example, quantitative trait loci (QTLs)) for identifying functional pathways and complex disease genes. Despite recent progress in QTLs, we expect that mutagenesis is will ultimately prove more effective because the prospects of gene identification are high and every gene affecting a trait is potentially a target. 相似文献
984.
985.
986.
Amino-acid radicals play key roles in many enzymatic reactions. Catalysis often involves transfer of a radical character within the protein, as in class I ribonucleotide reductase where radical transfer occurs over 35 A, from a tyrosyl radical to a cysteine. It is currently debated whether this kind of long-range transfer occurs by electron transfer, followed by proton release to create a neutral radical, or by H-atom transfer, that is, simultaneous transfer of electrons and protons. The latter mechanism avoids the energetic cost of charge formation in the low dielectric protein, but it is less robust to structural changes than is electron transfer. Available experimental data do not clearly discriminate between these proposals. We have studied the mechanism of photoactivation (light-induced reduction of the flavin adenine dinucleotide cofactor) of Escherichia coli DNA photolyase using time-resolved absorption spectroscopy. Here we show that the excited flavin adenine dinucleotide radical abstracts an electron from a nearby tryptophan in 30 ps. After subsequent electron transfer along a chain of three tryptophans, the most remote tryptophan (as a cation radical) releases a proton to the solvent in about 300 ns, showing that electron transfer occurs before proton dissociation. A similar process may take place in photolyase-like blue-light receptors. 相似文献
987.
ATM phosphorylates p95/nbs1 in an S-phase checkpoint pathway 总被引:70,自引:0,他引:70
The rare diseases ataxia-telangiectasia (AT), caused by mutations in the ATM gene, and Nijmegen breakage syndrome (NBS), with mutations in the p95/nbs1 gene, share a variety of phenotypic abnormalities such as chromosomal instability, radiation sensitivity and defects in cell-cycle checkpoints in response to ionizing radiation. The ATM gene encodes a protein kinase that is activated by ionizing radiation or radiomimetic drugs, whereas p95/nbs1 is part of a protein complex that is involved in responses to DNA double-strand breaks. Here, because of the similarities between AT and NBS, we evaluated the functional interactions between ATM and p95/nbs1. Activation of the ATM kinase by ionizing radiation and induction of ATM-dependent responses in NBS cells indicated that p95/nbs1 may not be required for signalling to ATM after ionizing radiation. However, p95/nbs1 was phosphorylated on serine 343 in an ATM-dependent manner in vitro and in vivo after ionizing radiation. A p95/nbs1 construct mutated at the ATM phosphorylation site abrogated an S-phase checkpoint induced by ionizing radiation in normal cells and failed to compensate for this functional deficiency in NBS cells. These observations link ATM and p95/nbs1 in a common signalling pathway and provide an explanation for phenotypic similarities in these two diseases. 相似文献
988.
989.
A family of candidate taste receptors in human and mouse 总被引:32,自引:0,他引:32
The gustatory system of mammals can sense four basic taste qualities, bitter, sweet, salty and sour, as well as umami, the taste of glutamate. Previous studies suggested that the detection of bitter and sweet tastants by taste receptor cells in the mouth is likely to involve G-protein-coupled receptors. Although two putative G-protein-coupled bitter/sweet taste receptors have been identified, the chemical diversity of bitter and sweet compounds leads one to expect that there is a larger number of different receptors. Here we report the identification of a family of candidate taste receptors (the TRBs) that are members of the G-protein-coupled receptor superfamily and that are specifically expressed by taste receptor cells. A cluster of genes encoding human TRBs is located adjacent to a Prp gene locus, which in mouse is tightly linked to the SOA genetic locus that is involved in detecting the bitter compound sucrose octaacetate. Another TRB gene is found on a human contig assigned to chromosome 5p15, the location of a genetic locus (PROP) that controls the detection of the bitter compound 6-n-propyl-2-thiouracil in humans. 相似文献
990.
Genomic rearrangement in NEMO impairs NF-kappaB activation and is a cause of incontinentia pigmenti. The International Incontinentia Pigmenti (IP) Consortium 总被引:12,自引:0,他引:12
Smahi A Courtois G Vabres P Yamaoka S Heuertz S Munnich A Israël A Heiss NS Klauck SM Kioschis P Wiemann S Poustka A Esposito T Bardaro T Gianfrancesco F Ciccodicola A D'Urso M Woffendin H Jakins T Donnai D Stewart H Kenwrick SJ Aradhya S Yamagata T Levy M Lewis RA Nelson DL 《Nature》2000,405(6785):466-472