Heritable and inducible genetic interference by double-stranded RNA encoded by transgenes

Double-stranded RNA interference (RNAi) is an effective method for disrupting expression of specific genes in Caenorhabditis elegans and other organisms. Applications of this reverse-genetics tool, however, are somewhat restricted in nematodes because introduced dsRNA is not stably inherited. Another difficulty is that RNAi disruption of late-acting genes has been generally less consistent than that of embryonically expressed genes, perhaps because the concentration of dsRNA becomes lower as cellular division proceeds or as developmental time advances. In particular, some neuronally expressed genes appear refractory to dsRNA-mediated interference. We sought to extend the applicability of RNAi by in vivo expression of heritable inverted-repeat (IR) genes. We assayed the efficacy of in vivo-driven RNAi in three situations for which heritable, inducible RNAi would be advantageous: (i) production of large numbers of animals deficient for gene activities required for viability or reproduction; (ii) generation of large populations of phenocopy mutants for biochemical analysis; and (iii) effective gene inactivation in the nervous system. We report that heritable IR genes confer potent and specific gene inactivation for each of these applications. We suggest that a similar strategy might be used to test for dsRNA interference effects in higher organisms in which it is feasible to construct transgenic animals, but impossible to directly or transiently introduce high concentrations of dsRNA.     

一种新型的CPU故障检测方法及其应用

本文根据国产新型单路数字新闻传真系统的特点,采用一种新颖而有效的 CPU 故障诊断算法.此方法的故障覆盖率较高,在程序运行后,得到令人满意的效果.     

关于两类n阶变系数线性方程的求解

众所周知,能用初等积分法求解的常微方程为数是不多的。就是线性方程,当系数是自变量的函数时,至今为止也没有一般的求解方法,但是当系数是常数时,便可求解了。本文对两类变系数方程,通过变量替换,化为常系数方程,从而解决了求解问题。     

酶性DNA（Ⅲ）：鱼精DNA酯酶活性的初步研究

从鱼精提取的ＤＮＡ表现酯酶活性。用α或β萘酯作底物，坚牢蓝作显色偶合剂，发现萘酯能被此ＤＮＡ水解从而导致坚牢蓝出现特征性的紫红色。ＤＮＡ溶液中的Ｈ＾＋或ＯＨ＾－并不能使坚牢蓝显色。     

合成气制备二甲醚工艺条件的研究

在固定床高压流动反应装置上考察了CuO -ZnO/HZSM - 5和CuO -ZnO -ZrO2 -MnO2 -Al2 O3/HZSM - 5二甲醚 (合成 )催化剂的活性 .实验结果表明 ,CuO -ZnO -ZrO2 -MnO2 -Al2 O3/HZSM - 5催化剂在高空速下活性较好 .同时确定二甲醚 (合成 )催化剂的最佳反应条件为 :T反 ≤ 2 5 0℃ ,P =4.0MPa,GHSV =1 0 0 0h- 1 .     

生态风险评价

本文阐述了生态风险评价的基本概念和定量研究方法．通过研究方法与技术路线,确立评价要素和受体—端点,建立一套风险评价的生态系统指标体系     

表面活性剂对Ni-P-PTFE复合镀液的影响

本文应用zeta电位仪研究了各种阳离子表面活性剂 ,非离子表面活性剂对Ni-P-PTFE复合镀中PTFE粒子zeta电位的影响 ,且应用扫描电子显微镜分析了镀层的形貌 结果表明 ,粒子的zeta电位越正 ,粒子在槽液中越不易聚结 ,且越有利于粒子的共沉积     

马氏环境中马氏链于小柱集上之概率的指数估计

本文给出了马氏随机环境中马氏链于小柱集上之概率的一个指数估计式，其估计指数即为熵，这一结果拓广了平稳遍历马氏链的相应结果。     

底层大空间高层结构自由振动的模态综合解法

本文将模态综合法用于底层大空间高层建筑结构自由振动的计算,导出了框支剪力墙—落地剪力墙结构,框支剪力墙—落地剪力墙—壁式框架结构自由振动的计算公式,文末给出了算例.     

粉粒体气力输送垂直管的压力损失

在作者工作的基础上,对不同的固体粒子在气流悬浮下垂直管内流动阻力进行了分析和实验研究。文中提出了固体粒子稀相流垂直管内适用的压力损失计算式。