核桃JrHSP20-1基因鉴定及温度胁迫响应功能分析

探讨核桃(Juglans regia)热激蛋白响应温度胁迫的分子调控机理,为核桃防寒工作等提供理论指导.通过对核桃‘香玲’转录组分析获得1个热激蛋白家族基因HSP20(命名为JrHSP20-1)的全长cDNA序列.采用实时荧光定量PCR(qRT-PCR)技术分析在40,44,48,16,10,6℃胁迫下JrHSP20-1在核桃根、茎、叶中的表达.并将JrHSP20-1构建酵母表达载体PYES2-JrHSP20-1进行温度胁迫功能分析.结果显示,JrHSP20-1基因全长891bp,编码的蛋白分子量为33.57KD,含296个氨基酸,理论等电点为5.07.表达分析显示,JrHSP20-1在不同组织均有不同程度的表达;根中,44℃时JrHSP20-1被诱导为对照的2.3倍,48℃时达对照的74.5倍,10℃时被诱导为对照的12.0倍;茎中,48℃时上调最大为对照的300.2倍;叶中,在48℃和16℃胁迫下被诱导较高,分别为对照的82.1,53.8倍.酵母温度胁迫实验发现,在53℃和-20℃胁迫下,重组酵母INVSC1(pYES2-JrHSP20-1)的生命活力及恢复活性明显优于对照酵母INVSC1(pYES2).得出核桃JrHSP20-1基因的表达具有一定的组织特性,响应于温度胁迫诱导,且JrHSP20-1基因能有效提高转基因酵母对高温和低温的耐受力,表明JrHSP20-1基因是核桃抗寒耐高温的有效候选基因,为进一步研究JrHSP20-1的抗寒功能打下基础.     

半群PO_n(k,m)的秩

设POn是[n]上的部分保序变换半群.对任意1≤k≤n-1且2≤m≤n,研究半群POn(k,m)={α∈POn:x,y∈dom(α),x≤k■xα≤k,y≥m■yα≥m}证明了半群POn(k,m)的幂等元秩为3n-4.进一步,得到了半群POn(k,k+1)的秩为2n-2,且半群POn(k,m)(m≠k+1)的秩为2n-1.     

Study of Products Distilled Spirits with Cassava Dregs

In this paper, Cassava dregs are an outgrowth produced during starchy production which uses cassava as raw material. It is usually dropped out or used as cheap feedstuff. In order to make the best use of cassava dregs, increase industries＇ benefits and reduce castoffthis study developed a ＇new technique which used cassava dregs as raw material to produce distilled spirits based on cassava dregs characteristics. The technique adopt solid-ferment procedure. At first, the ferment is processed by solid-state distilling, and then rectification extra care refinement; at last the tequila was produced with characteristics of simple and elegant fragrance and mellow-tasting.     

Absolute scaling law for temperature data in Rayleigh-Benard convection

In addition to the hierarchical-structure (H-S) model,this paper further explores the most intensive intermittent structure of Rayleigh-Bénard convection at the high Ra numbers proportional to temperature.With respect to the discovery and by means of the scale,both of Bolgiano,there are two regions of the structure holding the absolute scaling law given by Ching's paper.Through theoretic analysis of data,this paper indicates that the regions act as two local intensive intermittent structures,by which the st...     

Numerical simulations of self-propelled swimming of 3D bionic fish school

Numerical simulations of self-propelled swimming of a three dimensional bionic fish and fish school in a viscous fluid are carried out.This is done with the assistance of a parallel software package produced for 3D moving boundary problems.This computational fluid dynamics package combines the adaptive multi-grid finite volume method,the immersed boundary method and VOF (volume of fluid) method.By using the package results of the self-propelled swimming of a 3D bionic fish and fish school in a viscous fluid...     

Distributed Estimation in Sensor Networks with Repetition Coding

This paper considers the distributed estimation of a source parameter using quantized sensor observations in a wireless sensor network with noisy channels. Repetition codes are used to transmit quantization bits of sensor observations and a quasi best linear unbiased estimate is constructed to estimate the source parameter. Simulations show that the estimation scheme achieves a better power and spectral efficiency than the previous scheme.     

3-D Design and Numerical Simulation of Two-Phase Flow in the Laser Rapid Prototyping Coaxial Powder Delivery System

The metal powder direct and rapid prototyping technology is one of the most developing methods in the laser rapid prototyping technique. The coaxial powder delivery system is the key technique. The purpose of this paper is to introduce a new type of coaxial powder delivery system and simulate the metal powder and shield gas flow in the powder nozzle. 2-D and 3-D model of the new coaxial powder delivery system are established. Then gas-solid two-phase flow model and the k-ε turbulent model are selected to simulate the flow of metal powder in powder nozzle. The Euler-Lagrange method is used in the simulating computation. The results show that the new coaxial powder delivery system has stable performance, uniform powder flux, high cooling efficiency, and long useful life.     

MC-CDMA系统中的信道编码技术

将正交频分复用 (OFDM)与码分多址 (CDMA)相结合 ,构成MC CDMA系统。在简单讲述卷积码和Turbo码的编译码器结构的基础上 ,将这两种编码方案应用于MC CDMA系统的下行链路中 ,并进行了计算机模拟。     

河南省重点国有企业财务管理状况透析

河南省重点国有企业财务管理应尽快在实现政企完全分开，确立企业的市场主体地位的前提下，建立健全激励机制，拓宽筹资源道，加强对上市公司的监管等。