Construction and anti-tumor effects of recombinant fowlpox virus expressing Newcastle disease virus hemagglutinin-neuramidinase gene

Hemagglutinin-neuramidinase (HN), a Newcastle disease virus-derived protein, not only mediates receptor recognition but also possesses neuraminidase (NA) activity, the ability to cleave a component of those receptors, N-acetylneuraminic acid (NAcneu, sialic acid). It is known that this protein in mammalian species, including human beings, has interesting anti-neoplastic as well as immune stimulating properties. To explore the use of the HN gene in cancer gene therapy, we constructed a recombi-nant fowlpox virus expressing the HN protein (vFV-HN) and compared the anti-tumor activity of the recombinant virus with that of wild-type fowlpox virus (FPV) in vivo and in vitro. Here we found that although B16 cells were somewhat resistant to the basal cytotoxic effect of wild-type fowlpox virus, infection with vFV-HN caused a pronounced cytotoxic effect and, the survival of tumor-bearing mice immunized with vFV-HN was significantly increased compared with the survival of mice immunized with the FPV alone. Furthermore, the immunization of mice with vFV-HN elicited a B16 tumor-specific cytotoxic T lymphocyte (CTL) response and clonal expansion of both CD4 and CD8 T cell populations in vivo. In addition, T cells from lymph nodes of mice vaccinated with vFV-HN secreted high levels of the Th1 cytokine IL-2 and IFN-γ, indicating that the regression of tumor cells is related to a Th1-type dominant immune response. These results demonstrate that vaccination with vFV-HN may be a potential strategy for cancer gene therapy.     

快速介入性活检技术在纵隔疾病诊断中的应用

目的探讨B超引导下自制穿刺导向器快速介入性活检技术在纵隔疾病诊断中的临床应用价值。方法对36例经X线、CT或MRI证实有纵膈占位性病变且不能确诊的患者行自制B超穿刺导向器引导下的快速自动活检。结果36例患者全部穿刺成功,取材满意,取材阳性率100%。34例有明确的病理结果,病理阳性率94.4%。结论B超引导下自制穿刺导向器的快速介入性活检技术所取组织能够充分满足病理切片需要,病理确诊阳性率高,无严重并发症,能够为临床提供可靠的诊断治疗依据。     

配网馈线自动化通信方式的分析

对基于光纤的一点对多点的多点共线通信方式和基于无线的一点对多点的点对点通信方式进行了分析研究，并得出了两种通信方式相应的优缺点，就配网馈线自动化数据通信的要求，提出了应按网络方式传输数据，应按连接方式传输数据，应采用面向对象方式的应用层协议，应采用国际标准化的应用层协议。就数据通信对馈线终端单元（FTU）的要求，提出了FTU单元应具有网络内核操作系统，应按客户／服务器模式实现数据通信，应按面向对象方式定义组织传输的数据，应根据数据逻辑地址传输数据。     

锁相频率合成功率源与晶体介质谐振腔匹配网络

论述了锁模激光的调制器晶体谐振腔与锁相频率合成功率源匹配网络的实现，笔者经过深入研究，提出锁模激光的调制器晶体谐振腔非线性特性，并根据定一论断选择新的器件、网络电路，精心调制完成了难于实现的匹配，给出了实际运用的匹配波形图。     

浅层渗碳工艺方法研究及应用

薄板零件的浅层渗碳工艺为冲压成形零件提供了可靠的表面强化手段 ,具有独特的优点 ,工件经渗碳后其表面强度和耐磨性大大提高 ,同时由于心部和表面层含碳量不同 ,硬化后的表面获得有利的残余压应力 ,从而可进一步提高渗碳零件的弯曲疲劳强度和接触疲劳强度 介绍一种浅层渗碳的优化工艺 ,即采用低温、低碳势、渗碳后直接淬火的工艺 与传统的渗碳工艺相比 ,该工艺具有节能、省时 ,并且渗碳零件质量均匀、综合性能高的优点 ,充分发挥了低碳材料的潜力     

对称应力模型的一个疑难

应力对称模型对显著应力梯度问题，显著微结构介质，对波长较小的动力学问题，已经表现出与实验结果的显著差异。就各向同性线弹性均匀固体的静力学问题，介绍应力对称模型的一个疑难，对于直角形区域角顶的两边承受不等的剪应力问题，对称应力模型无解。引入非对称应力模型和偶应力化解了这一疑难。分析表明，随着到边界距离的增加，非对称应力模型的解趋近于对称应力模型的解。     

继电保护远方测试方法的探讨

就电力系统继电保护现场测试的不足，对电力系统继电保护远方测试的方法进行了探讨研究，给出了建立保护测试分析中心的技术要求、技术难点和制约因素，提出了建立电力系统继电保护测度分析中心的方法。就电力系统继电保护测试数据传输的问题，介绍了利用调度自动化系统数据网络来传输保护测试数据的技术要求。就电力系统继电保护远方测 方法对微机继电保护装置的要求，提出了现场微机继电保护装置应具有远方测试功能和具有同步测试功能。     

第四类华罗庚域上的Bergman核函数

显式给出了第四类华罗庚域HEⅣ上的Bergman核函数及其全纯自同构群。     

Modeling studies on the changes of the Subtropical High over the Western Pacific during the last 100 years

Intensity index series of Subtropical High over the Western Pacific was constructed according to modeling results by using of CCM3.6 atmospheric model with climatologically SST forcing for 30 years, and with observed SST forcing from 1900 to 2000. The index series constructed by modeling closely correlated to the observations for 1951-1999 (c.c. 0.72) and to the statistically reconstructed one for 1900-1950 (c.c. 0.51). The index series for 1900-1999 showed significant 80 a and 40 a periodicity. A minimum of persistence in seasonal variations of the index occurred in Autumn, which was called "Autumn Barrier". It may relate to the "Spring Barrier", found in the seasonal variations of SST over the Eastern Equatorial Pacific. The increasing trend shown in the index series for the 20th century may associate with the global warming caused by enhancement of greenhouse effect.     

辊压适张度对圆锯片模态参数的影响

采用实验模态分析的方法 ,研究了在不同适张度情况下圆锯片模态参数的变化规律 ,得到了圆锯片适张度的一种评价依据