The humoral immune response to heat shock proteins.

Humoral immune reactions to heat shock proteins (hsp) from microorganisms are one aspect of microbial infections in humans. The production of antibodies which are specific to epitopes present on procaryotic hsp leads also to the appearance of cross-reactive serum antibodies in the host organism that react with human hsp. This article discusses the consequences of such autoreactive antibodies for the host in context with the development of immune tolerance and autoimmune diseases, especially rheumatoid arthritis (RA), and in experimental animal models for arthritis such as adjuvant arthritis in rats. On the basis of epitope cross-reactivity between hsp and other host proteins, a hypothesis is presented for the development of autoimmune disease following the production of hsp-specific antibodies.     

灰铸铁的应力屈服曲面及其三维热弹塑性应力解析

对原有的灰铸铁件应力屈服曲面进行了合理的简化,使其在理论上合理,工程应用上更为方便。建立了灰铸铁件三维非稳态热弹塑性应力场的有限元方法。以灰铸铁哑铃件为对象,用新屈服曲面对其应力发展过程进行了模拟,且比较了用新旧屈服面计算得到的结果。     

中药材中孳生粉螨的初步调查

本文采用清水漂浮法和塔氏电热集螨器分离法,共分离中药材样本124种,1240份,从中分离出粉螨45种,隶属7科23属.得出结论:中药材粉螨的污染严重,应加强对中药螨类的防治,以保护中药材及预防人体螨病.     

Phenological studies of selected savanna mosses of south-western Nigeria

It was established that there is little diversity of bryophytes in the derived savanna. Mosses were found in the sampling sites, whereas liverworts were rarely observed. The reproductive methods of four dominant sexually reproducing savanna mosses —Archidium ohioense, Bryum coronatum, Fissidens minutifolius andTrachycarpidium tisserantii were monitored over two consecutive rainy seasons. Protonemal and gametophyte production were noticed in the field in March/April, and capsule dehiscence and spore dispersal occurred in September/October. The sequential stages of development, starting with gametangial production and ending with the falling of the dehisced capsules, occurred within the rainy season. However,A. ohioense andT. tisserantii did not discharge their spores easily (cleistocarpous), unlike the stegocarpous speciesB. coronatum andF. minutifolius. Water availability and possibly high humidity may have contributed to growth. The short period between sex organ formation and dehiscence of capsule seen in these studies, compared with the longer period in some temperate mosses, may be an advantage for bryophytes in a savanna environment.     

提高大型仪器设备利用率的新途径

大型仪器设备是高校开展教学、科研的物质保证,是高校实验室的主要技术装备,衡量一所高校办学实力和水平的重要指标之一.……     

Brdicka溶液中β_2—微球蛋白和巯基乙酸的检测

本文首次研究了β_2—微球蛋白和巯基乙酸在Brdicka溶液中产生灵敏“活性钴”催化电流的条件,并在此基础上分别测定了二者的最低检测限和线性范围。实验表明:利用该催化电流进行伏安免疫检测(VIA),其灵敏度将不亚于传统的放射免疫检测(RIA),同时,该法还具有简单、快速、可均相测定的优点。     

Exocytotic fusion is activated by Rab3a peptides.

Studies of intracellular traffic in yeast and mammalian systems have implicated members of the Rab family of small GTP-binding proteins as regulators of membrane fusion. We have used the patch clamp technique to measure exocytotic fusion events directly and investigate the role of GTP-binding proteins in regulating exocytosis in mast cells. Intracellular perfusion of mast cells with GTP-gamma S is sufficient to trigger complete exocytotic degranulation in the absence of other intracellular messengers. Here we show that GTP is a potent inhibitor of GTP-gamma S-induced degranulation, indicating that sustained activation of a GTP-binding protein is sufficient for membrane fusion. We have found that synthetic oligopeptides, corresponding to part of the effector domain of Rab3a, stimulate complete exocytotic degranulation, similar to that induced by GTP-gamma S. The response is selective for Rab3a sequence and is strictly dependent on Mg2+ and ATP. This suggests that sustained activation of a Rab3 protein causes exocytotic fusion. The peptide response can be accelerated by GDP-beta S, suggesting that Rab3a peptides compete with endogenous Rab3 proteins for a binding site on a target effector protein, which causes fusion on activation.     

Influence of hydrochlorothiazide on the pain threshold and on the antinociceptive activity of morphine,in rats

Summary Hydrochlorothiazide, acutely injected in rats, has a weak analgesic activity per se and potentiates and prolongs the antinociceptive effect of morphine.This work was supported in part by grants from Consiglio Nazionale delle Ricerche, and by Ministero della Pubblica Istruzione, Roma.