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排序方式: 共有302条查询结果,搜索用时 31 毫秒
121.
Locasale JW Grassian AR Melman T Lyssiotis CA Mattaini KR Bass AJ Heffron G Metallo CM Muranen T Sharfi H Sasaki AT Anastasiou D Mullarky E Vokes NI Sasaki M Beroukhim R Stephanopoulos G Ligon AH Meyerson M Richardson AL Chin L Wagner G Asara JM Brugge JS Cantley LC Vander Heiden MG 《Nature genetics》2011,43(9):869-874
Most tumors exhibit increased glucose metabolism to lactate, however, the extent to which glucose-derived metabolic fluxes are used for alternative processes is poorly understood. Using a metabolomics approach with isotope labeling, we found that in some cancer cells a relatively large amount of glycolytic carbon is diverted into serine and glycine metabolism through phosphoglycerate dehydrogenase (PHGDH). An analysis of human cancers showed that PHGDH is recurrently amplified in a genomic region of focal copy number gain most commonly found in melanoma. Decreasing PHGDH expression impaired proliferation in amplified cell lines. Increased expression was also associated with breast cancer subtypes, and ectopic expression of PHGDH in mammary epithelial cells disrupted acinar morphogenesis and induced other phenotypic alterations that may predispose cells to transformation. Our findings show that the diversion of glycolytic flux into a specific alternate pathway can be selected during tumor development and may contribute to the pathogenesis of human cancer. 相似文献
122.
Cancellation of cellular responses to nanoelectroporation by reversing the stimulus polarity 总被引:1,自引:1,他引:0
Andrei G. Pakhomov Iurii Semenov Shu Xiao Olga N. Pakhomova Betsy Gregory Karl H. Schoenbach Jody C. Ullery Hope T. Beier Sambasiva R. Rajulapati Bennett L. Ibey 《Cellular and molecular life sciences : CMLS》2014,71(22):4431-4441
Nanoelectroporation of biomembranes is an effect of high-voltage, nanosecond-duration electric pulses (nsEP). It occurs both in the plasma membrane and inside the cell, and nanoporated membranes are distinguished by ion-selective and potential-sensitive permeability. Here we report a novel phenomenon of bioeffects cancellation that puts nsEP cardinally apart from the conventional electroporation and electrostimulation by milli- and microsecond pulses. We compared the effects of 60- and 300-ns monopolar, nearly rectangular nsEP on intracellular Ca2+ mobilization and cell survival with those of bipolar 60 + 60 and 300 + 300 ns pulses. For diverse endpoints, exposure conditions, pulse numbers (1–60), and amplitudes (15–60 kV/cm), the addition of the second phase cancelled the effects of the first phase. The overall effect of bipolar pulses was profoundly reduced, despite delivering twofold more energy. Cancellation also took place when two phases were separated into two independent nsEP of opposite polarities; it gradually tapered out as the interval between two nsEP increased, but was still present even at a 10-µs interval. The phenomenon of cancellation is unique for nsEP and has not been predicted by the equivalent circuit, transport lattice, and molecular dynamics models of electroporation. The existing paradigms of membrane permeabilization by nsEP will need to be modified. Here we discuss the possible involvement of the assisted membrane discharge, two-step oxidation of membrane phospholipids, and reverse transmembrane ion transport mechanisms. Cancellation impacts nsEP applications in cancer therapy, electrostimulation, and biotechnology, and provides new insights into effects of more complex waveforms, including pulsed electromagnetic emissions. 相似文献
123.
1686年,莱布尼茨在他的《形而上学谈》中指出,如果某个任意复杂理论是被允许的话,那么"理论"的观念就是个空观念,因为总是会有一种理论存在的。这种思想现在被发展成现代的算法信息论,这种理论所涉及的是计算机程序的大小并为哥德尔不完全性工作以及图灵的不可计算性的工作提供了一个新视角。尤其重要的是,停机概率欧米茄数,其比特位是不可还原的,也就是说,其最大值是不可知的数学事实。更一般地说,这些思想构成了一类"数字哲学",由Edward Fredkin,Stephen Wolfram以及其他人所倡导的,他们将世界视为一台巨大的计算机。最近还有人将其进行"数字物理学"的思辨,认为宇宙实际上是离散的而非连续的。这一世界系统在作者本人的《元数学》一书中作了简要论述。 相似文献
124.
MicroRNA silencing through RISC recruitment of eIF6 总被引:1,自引:0,他引:1
Chendrimada TP Finn KJ Ji X Baillat D Gregory RI Liebhaber SA Pasquinelli AE Shiekhattar R 《Nature》2007,447(7146):823-828
125.
Hara MR Kovacs JJ Whalen EJ Rajagopal S Strachan RT Grant W Towers AJ Williams B Lam CM Xiao K Shenoy SK Gregory SG Ahn S Duckett DR Lefkowitz RJ 《Nature》2011,477(7364):349-353
The human mind and body respond to stress, a state of perceived threat to homeostasis, by activating the sympathetic nervous system and secreting the catecholamines adrenaline and noradrenaline in the 'fight-or-flight' response. The stress response is generally transient because its accompanying effects (for example, immunosuppression, growth inhibition and enhanced catabolism) can be harmful in the long term. When chronic, the stress response can be associated with disease symptoms such as peptic ulcers or cardiovascular disorders, and epidemiological studies strongly indicate that chronic stress leads to DNA damage. This stress-induced DNA damage may promote ageing, tumorigenesis, neuropsychiatric conditions and miscarriages. However, the mechanisms by which these DNA-damage events occur in response to stress are unknown. The stress hormone adrenaline stimulates β(2)-adrenoreceptors that are expressed throughout the body, including in germline cells and zygotic embryos. Activated β(2)-adrenoreceptors promote Gs-protein-dependent activation of protein kinase A (PKA), followed by the recruitment of β-arrestins, which desensitize G-protein signalling and function as signal transducers in their own right. Here we elucidate a molecular mechanism by which β-adrenergic catecholamines, acting through both Gs-PKA and β-arrestin-mediated signalling pathways, trigger DNA damage and suppress p53 levels respectively, thus synergistically leading to the accumulation of DNA damage. In mice and in human cell lines, β-arrestin-1 (ARRB1), activated via β(2)-adrenoreceptors, facilitates AKT-mediated activation of MDM2 and also promotes MDM2 binding to, and degradation of, p53, by acting as a molecular scaffold. Catecholamine-induced DNA damage is abrogated in Arrb1-knockout (Arrb1(-/-)) mice, which show preserved p53 levels in both the thymus, an organ that responds prominently to acute or chronic stress, and in the testes, in which paternal stress may affect the offspring's genome. Our results highlight the emerging role of ARRB1 as an E3-ligase adaptor in the nucleus, and reveal how DNA damage may accumulate in response to chronic stress. 相似文献
126.
Carpten JD Faber AL Horn C Donoho GP Briggs SL Robbins CM Hostetter G Boguslawski S Moses TY Savage S Uhlik M Lin A Du J Qian YW Zeckner DJ Tucker-Kellogg G Touchman J Patel K Mousses S Bittner M Schevitz R Lai MH Blanchard KL Thomas JE 《Nature》2007,448(7152):439-444
Although AKT1 (v-akt murine thymoma viral oncogene homologue 1) kinase is a central member of possibly the most frequently activated proliferation and survival pathway in cancer, mutation of AKT1 has not been widely reported. Here we report the identification of a somatic mutation in human breast, colorectal and ovarian cancers that results in a glutamic acid to lysine substitution at amino acid 17 (E17K) in the lipid-binding pocket of AKT1. Lys 17 alters the electrostatic interactions of the pocket and forms new hydrogen bonds with a phosphoinositide ligand. This mutation activates AKT1 by means of pathological localization to the plasma membrane, stimulates downstream signalling, transforms cells and induces leukaemia in mice. This mechanism indicates a direct role of AKT1 in human cancer, and adds to the known genetic alterations that promote oncogenesis through the phosphatidylinositol-3-OH kinase/AKT pathway. Furthermore, the E17K substitution decreases the sensitivity to an allosteric kinase inhibitor, so this mutation may have important clinical utility for AKT drug development. 相似文献
127.
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129.
Intestinal tissue mass was significantly reduced throughout the gastrointestinal tract (p less than 0.001) of intravenously fed (TPN) rats. Urogastrone-epidermal growth factor, (URO-EGF), reversed these changes. Although plasma enteroglucagon and gastrin levels showed a small increase with URO-EGF, this was far less than the gut tissue weight change, suggesting that it was unlikely that they were involved in modulating the proliferative response of the intestine to URO-EGF. Peptide tyrosine tyrosine (PYY) levels were however significantly increased by URO-EGF, indicating that PYY may possibly have a role in the modulation of intestinal cell proliferation. 相似文献
130.