经典力学

全书分为四部分，含19章1个附录。第一部分单质点的牛顿力学，含第1～8章。第1章代数和向量的运算；第2章速度、加速度和标量角速展；第3章牛顿运动定律和引力定律；第4章质点动力学中的问题；第5章线性振荡；第6章能量守恒；第7章有心场中的轨迹；第8章非线性振荡和相空间。第二部分多质点系统，含9～11章。第9章能量原理；第10章线性动量原理；第11章角动量原理。第三部分分析力学，含第12～14章。第12章拉格朗日方程和守恒原理；第13章变分的运算和哈密顿原理；第14章哈密顿方程和相空间。第四部分进一步的论题，含第15～19章。第15章小振荡的一般方程；第16章向量角速度和刚体运动学；第17章旋转参考标架；第18章张量代数和惯性张量；第19章刚体动力学中的问题。附录质量中心和惯性矩。最后对每一章后的习题给出了部分的解答。     

Effects of G-protein mutations on skin color

A new class of dominant dark skin (Dsk) mutations discovered in a screen of approximately 30,000 mice is caused by increased dermal melanin. We identified three of four such mutations as hypermorphic alleles of Gnaq and Gna11, which encode widely expressed Galphaq subunits, act in an additive and quantitative manner, and require Ednrb. Interactions between Gq and Kit receptor tyrosine kinase signaling can mediate coordinate or independent control of skin and hair color. Our results provide a mechanism that can explain several aspects of human pigmentary variation and show how polymorphism of essential proteins and signaling pathways can affect a single physiologic system.     

Plant genetics: gene transfer from parasitic to host plants

Plant mitochondrial genes are transmitted horizontally across mating barriers with surprising frequency, but the mechanism of transfer is unclear. Here we describe two new cases of horizontal gene transfer, from parasitic flowering plants to their host flowering plants, and present phylogenetic and biogeographic evidence that this occurred as a result of direct physical contact between the two. Our findings complement the discovery that genes can be transferred in the opposite direction, from host to parasite plant.     

Structural analysis of a eukaryotic sliding DNA clamp-clamp loader complex

Sliding clamps are ring-shaped proteins that encircle DNA and confer high processivity on DNA polymerases. Here we report the crystal structure of the five-protein clamp loader complex (replication factor-C, RFC) of the yeast Saccharomyces cerevisiae, bound to the sliding clamp (proliferating cell nuclear antigen, PCNA). Tight interfacial coordination of the ATP analogue ATP-gammaS by RFC results in a spiral arrangement of the ATPase domains of the clamp loader above the PCNA ring. Placement of a model for primed DNA within the central hole of PCNA reveals a striking correspondence between the RFC spiral and the grooves of the DNA double helix. This model, in which the clamp loader complex locks onto primed DNA in a screw-cap-like arrangement, provides a simple explanation for the process by which the engagement of primer-template junctions by the RFC:PCNA complex results in ATP hydrolysis and release of the sliding clamp on DNA.     

Two mitotic kinesins cooperate to drive sister chromatid separation during anaphase

During anaphase identical sister chromatids separate and move towards opposite poles of the mitotic spindle. In the spindle, kinetochore microtubules have their plus ends embedded in the kinetochore and their minus ends at the spindle pole. Two models have been proposed to account for the movement of chromatids during anaphase. In the 'Pac-Man' model, kinetochores induce the depolymerization of kinetochore microtubules at their plus ends, which allows chromatids to move towards the pole by 'chewing up' microtubule tracks. In the 'poleward flux' model, kinetochores anchor kinetochore microtubules and chromatids are pulled towards the poles through the depolymerization of kinetochore microtubules at the minus ends. Here, we show that two functionally distinct microtubule-destabilizing KinI kinesin enzymes (so named because they possess a kinesin-like ATPase domain positioned internally within the polypeptide) are responsible for normal chromatid-to-pole motion in Drosophila. One of them, KLP59C, is required to depolymerize kinetochore microtubules at their kinetochore-associated plus ends, thereby contributing to chromatid motility through a Pac-Man-based mechanism. The other, KLP10A, is required to depolymerize microtubules at their pole-associated minus ends, thereby moving chromatids by means of poleward flux.     

Climatology: threatened loss of the Greenland ice-sheet

The Greenland ice-sheet would melt faster in a warmer climate and is likely to be eliminated--except for residual glaciers in the mountains--if the annual average temperature in Greenland increases by more than about 3 degrees C. This could raise the global average sea-level by 7 metres over a period of 1,000 years or more. We show here that concentrations of greenhouse gases will probably have reached levels before the year 2100 that are sufficient to raise the temperature past this warming threshold.