alpha-globulin follicular fluid proteins within small and large bovine follicles.

Fluids from small (FF-S) and large (FF-L) bovine ovarian follicles were observed electrophoretically for differences in the alpha-globulin protein profile. FF-L possessed a fast migrating alpha-globulin 3 and a greater percentage of FF-L samples contained a higher number of alpha-globulin bands.     

Assembly of microarrays for genome-wide measurement of DNA copy number.

We have assembled arrays of approximately 2,400 BAC clones for measurement of DNA copy number across the human genome. The arrays provide precise measurement (s.d. of log2 ratios=0.05-0.10) in cell lines and clinical material, so that we can reliably detect and quantify high-level amplifications and single-copy alterations in diploid, polyploid and heterogeneous backgrounds.     

A screen of the complete protein kinase gene family identifies diverse patterns of somatic mutations in human breast cancer

We examined the coding sequence of 518 protein kinases, approximately 1.3 Mb of DNA per sample, in 25 breast cancers. In many tumors, we detected no somatic mutations. But a few had numerous somatic mutations with distinctive patterns indicative of either a mutator phenotype or a past exposure.