Knockdown of <Emphasis Type="Italic">MRP4</Emphasis> by lentivirus-mediated siRNA improves sensitivity to adriamycin in adriamycin-resistant acute myeloid leukemia cells

Chemotherapy remains the standard treatment for acute myeloid leukemia;however,the emergence of drug resistance is a major hurdle in the successful treatment of leukemia.The expression of multidrug resistance-associated protein 4(MRP4)induces re- sistance in the adriamycin-resistant acute myeloid leukemia cell line,K562/ADR.The aim of this study was to investigate whether knockdown of MRP4 by lentivirus-mediated siRNA could improve the sensitivity of K562/ADR cells to adriamycin.Five lenti- virus-mediated short hairpin RNAs(lv-shRNAs-MRP4)were designed to trigger the gene silencing RNA interference(RNAi) pathway.The efficiency of lentivirus-mediated siRNA infection into K562/ADR cells was determined using fluorescence mi- croscopy to observe lentivirus-mediated GFP expression.MRP4 expression in infected K562/ADR cells was evaluated by real- time PCR and Western blot analysis.The MTS assay was used to measure cell viability and flow cytometry was used to measure apoptosis.The transfection efficiency of K562/ADR cells was over 80 percent.The gene silencing efficacy of lv-shRNA1-MRP4 was superior to the other constructs.Infection of K562/ADR cells with lv-shRNA1-MRP4 led to strong inhibition of MRP4 mRNA and protein expression.Combined treatment with lv-shRNA1-MRP4 and adriamycin decreased cell growth and increased apoptosis compared to treatment with lv-shRNA1-MRP4 or adriamycin alone.These data indicate that in K562/ADR cells MRP4 is involved in drug resistance mechanisms and that lentivirus-mediated knockdown of MRP4 may enhance sensitivity to adriamycin.     

Changes in the power spectrum of electrical signals in maize leaf induced by osmotic stress

To quantify the characteristics of the power spectrum of plant electrical signals, we defined the following concepts:spectral edge frequency (SEF), spectral center frequency (SCF), power index (PI) and power spectral entropy (PSE). These parameters were used to examine and quantify changes in the power spectrum of electrical signals in maize leaves under osmotic stress. In the absence of osmotic stress, the SEF of the electrical signal in maize leaves was approx. 0.2 Hz and the SCF was approx. 0.1 Hz. The electrical signal in maize leaves was mainly a slow wave signal with a frequency of 0-0.1 Hz. After 2 h osmotic stress, the SEF and SCF of the electrical signal increased to higher frequencies. The proportion of the fast wave frequency also increased to 0.1-0.2 Hz, resulting in a dramatic increase in PSE. We also found that the changes in PSE and SCF were significantly correlated during osmotic stress. We propose that the changes in the PSE and SCF in maize leaves can be used as a sensitive signal indicating water deficit in leaf cells under osmotic stress. Thus, measurement of SCF or PSE of electrical signals in maize leaves could be used to develop early warning and rapid diagnosis techniques for the water demands of plants.     

基于FPGA面向多媒体处理的MPSoC

针对嵌入式单核处理器处理速度慢及主频提升受限等问题,提出了嵌入式双核处理器(two-cores embedded processor,TEP)模型.针对处理器运行时对存储器的依赖和分配问题,提出了基于非统一存储结构模拟分布式存储结构的方案;针对多核间对共享数据存储器的访存问题,给出了从属单元的仲裁机制,实现了共享资源的访问;针对面向多媒体应用的多核处理器间传输数据量大及通讯开销高的问题,提出了基于消息数据分离的传输方案.系统在FPGA平台进行了实现和验证,测试结果表明,TEP系统以较少的资源消耗和通讯开销获得了大加速比的性能.     

疏水冷面霜晶生长的有限扩散凝聚模型

为了深入了解霜晶的动态生长过程,以有限扩散凝聚模型为基础建立了疏水冷面霜晶二维生长模型.通过在表面上设置不同冰珠数量来模拟冷面不同疏水性能,无涂层金属表面则布满冰珠;采用粒子在网格节点上随机移动来描述水蒸气分子的运动,粒子沿各方向移动的概率相同.数值结果表明,霜晶动态生长的可视化结果与实验图像在形态上保持一致;树枝状霜晶具有分形特征,相应的分形维数为1.44～1.78,与实验值相近.疏水冷面霜晶生长缓慢且分布稀疏;采用疏水效果不好的冷面的结霜情况与金属表面相似.     

西藏旅游发展中的负外部效应及其内化研究

西藏旅游发展中负外部性表现在以下几个方面：一是季节性集中的大量游客导致的环境成本;二是旅游对藏民族传统文化生态的破坏引起的社会成本;三是旅游对西藏其他产业的＂挤出效应＂所导致的机会成本。文章认为促使外部效应内部化,减少西藏旅游外部效应的对策包括政府运用税收、补贴、押金、保证金等经济手段实现旅游企业外部成本内部化;合理界定旅游资源产权,防止＂公地悲剧＂发生;建立民族生态博物馆,实行民族文化＂前台、帷幕、后台＂开发与保护模式;对旅游项目进行综合成本—收益分析,追求旅游综合效益最大化。     

以二甲醚为还原剂的氮氧化物存储还原试验研究

试验研究了以Pt/Co-BaO/γ^- Al₂O₃为催化剂、二甲醚（DME）为还原剂的NOx储存还原（NSR）性能,以及还原时间和温度对NSR的影响规律,并通过程序升温脱附和程序升温表面反应分析了DME对其存储NOx热稳定性的影响.结果表明,在等温DME-NSR循环过程中,NOx的转化效率保持在60%~70%以上;DME能够促进催化剂中硝酸盐的分解和NOx的脱附,并还原NOx.     

A new improvement of TCP based on adaptive filtering

On the basis of studying the standard TCP retransmission mechanism,this paper proposes a method to adopt the theory of adaptive filtering in the field for the estimation of round trip time (RTT).Then t...     

双核金属钌羰基-亚硝基配位化合物Ru_2（NO）_2（CO）_n（n=8,7,6,5）的量子化学计算

采用BP86和MPW1PW91两种密度泛函理论方法对双核金属钌羰基-亚硝基配位化合物Ru2（NO）2（CO）n（n=8,7,6,5）进行了量子化学理论计算,优化后得到了19个单态和三态异构体,讨论了它们的结构和键的性质。得到的Ru2（NO）2（CO）8,Ru2（NO）2（CO）7,Ru2（NO）2（CO）6和Ru2（NO）2（CO）5的基态分别是C2v,C1,C2v和C1构型的单态。将Ru2（NO）2（CO）n（n=7,6,5）与同族铁的配合物Fe2（NO）2（CO）n（n=7,6,5）进行比较,发现二者的稳定构型特点不一致。     

基于机器学习的Cortex-M监控视频车型识别

为了解决高速公路环境下监控视频图像车型识别需要将海量视频数据上传计算机服务器中心,对所有的视频流图像进行结构化处理和车型识别,造成服务器中心数据压力大、计算任务重,对服务器性能要求高的问题。对此,提出一种基于机器学习的Cortex-M监控视频车型识别的方法。首先,将训练机训练好的六种车型的权值矩阵文件移植到前端Cortex-M核系列开发板上,采用开发板内嵌的CMSIS-NN网络函数库搭建与训练机相同的网络模型结构;同时采用开发板内嵌的CMSIS-DSP库加快图像处理速度,并对选择处理监控视频图像实现车型识别;实验结果表明,该方法平均识别率达到94.6%以上,与采用计算机进行识别相同,可见该方法能够缓解大量视频上传给服务器中心造成的压力,为高速公路环境下监控视频图像车型识别研究提供了一种可选择的方案。     

铁路突发事件应急资源调配的优化与决策

优化铁路应急救援资源的调配是降低突发事件人员伤亡、减少财产损失的重要途径。基于应急资源调配的特点及要求,建立应急资源调配时间最短的单目标模型,以及调配时间最短,且应急出救点最少的双目标模型;并运用分层序列法进行求解。研究表明,双目标模型优化可以减少应急出救点、节约救援成本。利用Matlab软件设计应急资源优化调配的决策程序,实现资源调配决策的快速化、科学化;并以南昌铁路局为例进行实证分析。研究成果为铁路突发事件的科学应急救援,减少事故救援时间和成本提供参考。