Gauss整环上的二维AFT算法

利用整环上的Ｍｂｉｕｓ反演公式，给出了Ｇａｕｓｓ整环上的二维Ｆｏｕｒｉｅｒ系数的ＡＦＴ算法，其方法同样适用于小波级数的系数计算。     

求可达矩阵的Warshall算法

给出并证明了确定内部独立的递阶层次结构的矩阵方法。将系统用有向图描述，利用集合论中求关系问包的Ｗａｒｓｈａｌｌ算法实现了求可达矩阵。在决策因素很多且问题很复杂时，可以通过有向图的可达矩阵来确定系统的层次结构。     

岩石含水饱和度,频率,流体类型对声波振幅影响规律的探讨

本实验以不同类型的砂岩为试样,在常温常压下用超声参数测定仪测量了饱和度、频率和流体类型对多孔隙岩石中声波衰减的影响,得到以下结论:(1)在干燥岩石中,纵波衰减比横波高;在被流体完全或部分饱和的岩石中,纵、横波的衰减均高于在干燥岩石中的衰减,横波的衰减的增加量比纵波大,并以复杂的方式依赖于饱和度。(2)在饱和盐水的岩石中,声波振幅随频率的增高而呈指数衰减。在低频段,声波有较大的衰减变化率;在某一特征频率(600kHz左右)之后,振幅与频率基本无关。(3)对同一块样品,饱和不同类型的流体,声波的振幅随流体的粘度增大而减小。     

短小芽孢杆菌质粒pCJ3的衍生质粒pAl32的酶谱及抗性基因定位

从短小芽孢杆菌四环素抗性质粒pCJ3截短的衍生质粒pAL32,经用6种限制酶双酶解试验,根据琼脂糖凝胶电泳带估算各片段的分子量,作出了pAL32的6种限制酶图谱。利用已除去复制功能的金黄色葡萄球菌质粒pUB110与pAL32构建的Km~rTc~r的双抗性重组质粒pSC33为载体,在EcoRV位点克隆的λDNA片段的重组子均为Km~rTc~s。用pUB110与pAL32在PvuⅡ位点连接后的重组质粒也为Km~rTc~s。根据这些重组子四环素抗性的失活,推知pAL32上EcoRV与PvuⅡ切点均位于其四环素抗性基因上。     

Self-Expanded Clustering Algorithm Based on Density Units with Evaluation Feedback Section

This paper presents an effective clustering mode and a novel clustering result evaluating mode. Clustering mode has two limited integral parameters. Evaluating mode evaluates clustering results and gives each a mark. The higher mark the clustering result gains, the higher quality it has. By organizing two modes in different ways, we can build two clustering algorithms： SECDU（Self-Expanded Clustering Algorithm based on Density Units） and SECDUF（Self-Expanded Clustering Algorithm Based on Density Units with Evaluation Feedback Section）. SECDU enumerates all value pairs of two parameters of clustering mode to process data set repeatedly and evaluates every clustering result by evaluating mode. Then SECDU output the clustering result that has the highest evaluating mark among all the ones. By applying ＂hill-climbing algorithm＂, SECDUF improves clustering efficiency greatly. Data sets that have different distribution features can be well adapted to both algorithms. SECDU and SECDUF can output high-quality clustering results. SECDUF tunes parameters of clustering mode automatically and no man＇s action involves through the whole process. In addition, SECDUF has a high clustering performance.     

机电传感器的特征化及其特征参数的确定

本文指出用Z参数和ABCD参数分别表示机电传感器在接收状态和发生状态下的网络参数是方便且合理的。然后本文规定接收器灵敏度和发生器发射电压和电流响应作为其相应的特征化参数。进而,本文提出一个三参数模型描述传感器的行为,并发展一个复数最小二乘拟合Fortran计算方法求出数学介。这样,机电传感器的网络参数和特征化参数可从它的一组电阻抗和负载阻抗数据对确定。     

Mismatch repair genes identified using genetic screens in Blm-deficient embryonic stem cells

Phenotype-driven recessive genetic screens in diploid organisms require a strategy to render the mutation homozygous. Although homozygous mutant mice can be generated by breeding, a reliable method to make homozygous mutations in cultured cells has not been available, limiting recessive screens in culture. Cultured embryonic stem (ES) cells provide access to all of the genes required to elaborate the fundamental components and physiological systems of a mammalian cell. Here we have exploited the high rate of mitotic recombination in Bloom's syndrome protein (Blm)-deficient ES cells to generate a genome-wide library of homozygous mutant cells from heterozygous mutations induced with a revertible gene trap retrovirus. We have screened this library for cells with defects in DNA mismatch repair (MMR), a system that detects and repairs base-base mismatches. We demonstrate the recovery of cells with homozygous mutations in known and novel MMR genes. We identified Dnmt1(ref. 5) as a novel MMR gene and confirmed that Dnmt1-deficient ES cells exhibit micro-satellite instability, providing a mechanistic explanation for the role of Dnmt1 in cancer. The combination of insertional mutagenesis in Blm-deficient ES cells establishes a new approach for phenotype-based recessive genetic screens in ES cells.     

Change in the body temperature of healthy term infant over the first 72 hours of life

OBJECTIVE: To determine the range of body temperature in a group of healthy Chinese term neonates over the first 72 hours of life and to assess the influence of body weight, gestational age and route of delivery.METHOD: All 200 consecutive cases of neonates delivered at our hospital from March to August 2001 were included in this retrospective study. Temperatures were measured immediately after delivery, after 30 minutes, 1 hour, 2 hours, 8 hours and 15 hours and on the 2nd and 3rd day. Axillary temperatures ranging from 36.5 degrees C to 37 degrees C were regarded as normal. No cases of maternal fever or systemic infection of the newborns were discovered. All infants were discharged in good general condition. RESULTS: The mean rectal temperature at birth was 37.19 degrees C. The lowest average temperature was reached at 1 hour after delivery (36.54 degrees C) with a significant difference between natural delivery (36.48 degrees C) and section (36.59 degrees C) (P<0.05). Temperature subsequently rose to 36.70 degrees C at 8 hours and 36.78 degrees C at 15 hours (P<0.05). Hypothermia was seen in 51.8% and hypothermia in 42.5% of the patients. On the 3rd day after delivery, 96% of all temperatures were in the normal range. A significant relation was found between hypothermia and both low birth weight (P<0.001) and low gestational age (P<0.05). CONCLUSION: The reference range presently used did not include all physiological temperatures in the first 72 hours of life. Considering other factors, such as birth weight, route of delivery, gestational age and body temperature on the 2nd and 3rd day of life, may help to correctly assess the significance of temperatures beyond the reference range.     

TRPV3 is a calcium-permeable temperature-sensitive cation channel

Transient receptor potential (TRP) proteins are cation-selective channels that function in processes as diverse as sensation and vasoregulation. Mammalian TRP channels that are gated by heat and capsaicin (>43 degrees C; TRPV1 (ref. 1)), noxious heat (>52 degrees C; TRPV2 (ref. 2)), and cooling (< 22 degrees C; TRPM8 (refs 3, 4)) have been cloned; however, little is known about the molecular determinants of temperature sensing in the range between approximately 22 degrees C and 40 degrees C. Here we have identified a member of the vanilloid channel family, human TRPV3 (hTRPV3) that is expressed in skin, tongue, dorsal root ganglion, trigeminal ganglion, spinal cord and brain. Increasing temperature from 22 degrees C to 40 degrees C in mammalian cells transfected with hTRPV3 elevated intracellular calcium by activating a nonselective cationic conductance. As in published recordings from sensory neurons, the current was steeply dependent on temperature, sensitized with repeated heating, and displayed a marked hysteresis on heating and cooling. On the basis of these properties, we propose that hTRPV3 is thermosensitive in the physiological range of temperatures between TRPM8 and TRPV1.     

加强实践教学提高人才培养质量

职业教育应重视培养学生的能力,必须加强实践教学,明确教学指导思想,强化实践教学环节,鼓励学生社会实践活动,抓好毕业实训工作。