Application of surface plasmon resonance in screening adsorbents and explaining adsorption phenomena using model polymers

A surface plasmon resonance (SPR) technique was developed as an online screening method to identify effective adsorbents for the peptide NH₂-Cys-Thr-Trp-Trp-COOH (CW-4). To validate the SPR technique, several linear polymers containing amino acid residues were synthesized as models of their corresponding adsorbents. SPR screening of all the linear polymers demonstrated that the linear polymer containing phenylalanine residues (PPhe) exhibited the highest affinity for CW-4 among the polymers tested. In accordance with the screening results from SPR, the adsorbent containing phenylalanine (APhe) exhibited the highest adsorption capacity for CW-4. An interesting observation was that the amounts of CW-4 adsorbed on 3 adsorbents were quite small, even though both SPR and isothermal titration calorimetry (ITC) experiments indicated the presence of interactions between CW-4 and the three polymers. A kinetic analysis performed using SPR technology suggested such a phenomenon was ascribed to the poor stability of the CW-4/polymer complexes; that is, CW-4 associates with the polymers quickly, but it also dissociated quickly. The combined results suggested that SPR was a promising tool to identify the optimal adsorbent for peptides, analyze the interactions contributing to adsorption and explain adsorption phenomena using polymer models.     

Banach空间一类非线性算子方程的不动点

利用锥理论和非对称迭代方法，讨论了不具有连续性和紧性条件的非线性算子方程A（x，x）＋u0=Bx解的存在唯一性，并给出了迭代序列收敛于解的误差估计，所得结果是某些已知结果本质改进和推广．     

以科学发展观统领区域高校工作全局

坚持用科学发展观统领区域高校工作全局,要全面正确地把握科学发展观的第一要义、核心内涵、基本要求、实现途径和根本方法,紧紧围绕发展"这一主题",坚持做到"两个为本",牢固树立"三个观点",切实做到"四个着力",辩证处理"十大关系",不断增强贯彻落实科学发展观的自觉性和坚定性.     

论企业文化建设

企业文化建设要想真正成功,必须结合企业自身特点和所属行业的特性,创建个性鲜明、富有特色的企业文化,文章对推进企业文化建设进行了论述.     

浅议会计的监督职能

文章对会计监督职能弱化的原因进行了分析，提出强化会计监督职能的相关对策。     

水电站尾水河道定床变态模型相似律

水电站尾水河道的水流几何形态变化得足够强烈或频繁时，尾水河道中水流单位 长度上的能量损失．主要是局部阻力损失．考虑水流局部能量损失，得到模型相似参 数。采用不同的方法证明了水力摸型试验成果的缩尺影响很小．水力模型试验成果与 原型观测资料比较，两者符合较好。     

萃取动力学分析法的研究和应用Ⅵ.非催化法测定Ce(Ⅳ)

利用Ce(Ⅳ)在2mol/LHCl 介质中氧化二苯硫腙褪色的慢反应,用萃取平衡控制反应时间和水相中二苯硫腙的浓度,建立了非催化动力学分析法测定Ce(Ⅳ)的新方法。反应速度的检测是在620nm下测定反应前后CCl_4相中二苯硫腙吸光度的变化。方法的检测限为5.0×10~(-7) gCe(Ⅳ)·ml~(-1) ,线性范围为0. 5-5μgCe(Ⅳ)·ml~(-1)。对干扰严重的金属离子 Ag~+、Au~(3+)、Cu~(2+)、Hg~(2+)等可在 pH4-6条件下,用二苯硫腙的四氯化碳溶液萃取除去。将方法用于测定合成水样中Ce(Ⅳ),结果满意。