Effect of thrombin on the apoptosis of hippocampal neurons in vitro

Hippocampal neurons were treated by thrombin and thrombin receptor activating peptides (TRAP). Cell survival rate was decreased in a dose-dependent manner by MTT assay. The numbers of apoptotic cell and apoptotic rate of hippocampal neurons treated by different concentrations of thrombin were increased in a dose-dependent manner by terminal deoxynucleotidyl transferase (TdT) mediated dUTP-biotin nick end-labeling (TUNEL) method and Flow Cytometry. When the concentration of thrombin is 40 U/mL, TUNEL positive cells and apoptotic rate of hippocampal neurons reached peak value, were 27.3±4.0 and (29.333±4.633)%, respectively. Immunocytochemistry assay show that Bcl-2 protein expression was down-regulated and Bax protein expression was up-regulated with the concentration of thrombin increased. TRAP can mimic the effect of thrombin to induce apoptosis on hippocampal neurons. These data demonstrated that thrombin induced hippocampal neuron apoptosis in a dose-dependent manner through activating protease-activated protein-1 (PAR-1). The change in expression of Bcl-2 and Bax was related with the effect of high concentration thrombin induced apoptosis on hippocampal neurons. Foundation item: Supported by the Natural Science Foundation of Hainan Province (N30215) Biography: YANG Wen-qiong (1968-), female, Ph.D. candidate, research direction: cerebrovascular disease.     

试用层次分析法确定疑难病症的治疗方案

医生在为疑难病症制定治疗方案时,涉及到患者病情、经济状况、治疗条件和费用等方面的因素,通常是以经验或主观判断为主,而数学在医学中的应用还不多为避免医生或患者的主观选择影响治疗,本文应用数学方法,将可以采用的治疗方案、医患者客观存在的条件及最后拟采用的治疗方案建立成层次结构模型,阐述了将层次结构模型构造成对比较阵、确定一致性检验指标、计算组合权向量及检验组合一致性的方法当因素和层次较多,计算工作量较大,特别是通不过一致性检验时,需重新调整、重建成对比较阵,进行反复计算,因此采用计算机编程对模型进行电算是必要的最后,例举了一个病例说明该方法在确定疑难病症治疗方案上的具体应用     

强阻尼半线性波动方程的指数吸引子

研究了一类带强阻尼项的半线性波动方程的指数吸引子的存在性,通过算子分解来构造渐近紧的不变吸收集,进而得到了空间Ｅ１中的指数吸引子     

硬实时任务在多处理机上的动态调度研究

在硬实时任务集能够在多处理机上进行静态调度的基础上,从理论上研究了相同硬实时任务集在多处理机上能够进行动态调度的条件,并给出了动态调度规则,为硬实时任务的动态调度问题提供了一种新的解决途径。     

A forensic method for efficient file extraction in HDFS based on three-level mapping

The large scale and distribution of cloud computing storage have become the major challenges in cloud forensics for file extraction. Current disk forensic methods do not adapt to cloud computing well and the forensic research on distributed file system is inadequate. To address the forensic problems, this paper uses the Hadoop distributed file system (HDFS) as a case study and proposes a forensic method for efficient file extraction based on three-level (3L) mapping. First, HDFS is analyzed from overall architecture to local file system. Second, the 3L mapping of an HDFS file from HDFS namespace to data blocks on local file system is established and a recovery method for deleted files based on 3L mapping is presented. Third, a multi-node Hadoop framework via Xen virtualization platform is set up to test the performance of the method. The results indicate that the proposed method could succeed in efficient location of large files stored across data nodes, make selective image of disk data and get high recovery rate of deleted files.     

基于小波变换的主元分析人脸图象识别

基于小波变换的主元分析人脸图象识别高西奇，周洪祥，何振亚（东南大学无线电工程系，南京２１００１８）人脸图象识别是模式识别领域的一个重要研究课题［１，２］，但是由于人脸虽有固定结构而无固定形状的特殊性，人脸的自动识别非常困难，特别是要求机器在学习之后能...     

Microstructural characteristics and mechanical properties of bobbin-tool friction stir welded 2024-T3 aluminum alloy

Cold-rolled 2024-T3 sheet alloy was subjected to bobbin-tool friction stir welding (BTFSW). The microstructural characteristics and mechanical properties of the nugget zone in the as-welded state were investigated. The results show that the equiaxed grain size of BTFSW 2024-T3 alloy decreases from 7.6 to 2.8 μm as the welding speed is increased from 80 to 120 mm/min; in addition, fine grains are generated in the nugget zone and the size distribution is non-uniform. All Al₂CuMg (S') precipitates dissolve into the Al matrix, whereas Mn-rich phases confirmed as T phases (Al₂₀Cu₂Mn₃, Al₆Mn, or Al₃Mn) remain unchanged. The optimized parameters for BTFSW are verified as the rotation speed of 350 r/min and the travel speed of 100 mm/min. The variations in precipitation and dislocation play more important roles than grain size in the nugget zone with respect to influencing the mechanical properties during the BTFSW process. After the BTFSW process, the fracture mode of base material 2024-T3 alloy transforms from ductile rupture to ductile-brittle mixed fracture.     

面向对象的活塞CAD/CAM述评

介绍了活塞CAD/CAM技术及其发展现状。该技术主要包括在三维有限元的基础上 ,采用面向对象的技术和专家知识型的数据库建立活塞的实体模型 ,并在进行有限元的剖分和边界条件的确立后对分析结果优化设计 ,最后将设计好的实体通过在计算机自动生成NC程序。活塞CAD/CAM是一套在三维实体建模基础上的CAD/CAM一体化系统     

FTIR assessment of the secondary structure of proteins in human breast benign and malignant tissues

The compositions of the secondary structures of protein in the human breast normal, hyperplasia, fibroadenoma and invasive ductal carcinoma tissues have been estimated from the Fourier self deconvolved spectra, the second derivative spectra and the curve-fitting analysis of the amide I bands in their spectra. Some parameters of the secondary structures of proteins in these 4 types of tissues are significantly different and located in separate ranges. Fibroadenoma tissue demonstrates the most remarkable difference in the compositions of α-helical and atypical helical structures. The carcinoma tissue has the highest ratio of the intermolecular β-sheet content and the intramolecular β-sheet content. This noticeable difference in the secondary structure may have important implications for analyzing the properties and types of human breast disease.     

优化Tikhonov迭代法在电容层析成像中的应用

为了提高电容层析成像的速度和质量,将收敛速度较快的Tikhonov迭代法应用于电容层析成像.Tikhonov迭代法的难点在于正则化系数的选取,通过对其正则化作用的分析,提出利用对灵敏场的奇异值分解,选取最大奇异值作为正则化系数,从而保证算法收敛的稳定性;同时为了提高收敛速度,将线性反演算法(LBP)计算所得的灰度作为迭代的初始值.结果表明:该正则化系数具有更高的稳定性和收敛速度;Tikhonov迭代法与Landweber迭代法相比具有收敛速度更快,重建图像质量更高的优点.