联结数与分数（k，n’）一临界消去图

设G是一个图,若删除G中任意n’个顶点的剩余子图依然是分数k-消去图,则称G为分数（k,n＇）-临界消去图．笔者证明了若k≥2,n,≥0,bind（G）≥^（n＇＋1）且6（G）≥k＋n＇＋1,则G是分数（k,n＇）-临界消去图．     

有向图和二部有向图的局部边连通性

笔者首先利用顶点的度和给出了有向图是超级局部边连通的一个最好可能的充分条件,然后提出了二部有向图为极大局部边连通和超级局部边连通的度序列条件.这些结果在网络可靠性分析中有一定应用.     

Re-emerging superconductivity at 48 kelvin in iron chalcogenides

Pressure has an essential role in the production and control of superconductivity in iron-based superconductors. Substitution of a large cation by a smaller rare-earth ion to simulate the pressure effect has raised the superconducting transition temperature T(c) to a record high of 55?K in these materials. In the same way as T(c) exhibits a bell-shaped curve of dependence on chemical doping, pressure-tuned T(c) typically drops monotonically after passing the optimal pressure. Here we report that in the superconducting iron chalcogenides, a second superconducting phase suddenly re-emerges above 11.5?GPa, after the T(c) drops from the first maximum of 32?K at 1?GPa. The T(c) of the re-emerging superconducting phase is considerably higher than the first maximum, reaching 48.0-48.7?K for Tl(0.6)Rb(0.4)Fe(1.67)Se(2), K(0.8)Fe(1.7)Se(2) and K(0.8)Fe(1.78)Se(2).     

靶向TTF-1的siRNA腺相关病毒载体组装验证

为了解决靶向TTF-1的siRNA导入肺腺癌细胞的问题,设计了3条靶向NCI-H1975细胞TTF-1基因的siRNA,并将其分别构建到腺相关病毒上.在293细胞中装配病毒,收获病毒原初液后进行超滤浓缩、层析柱纯化,测定病毒滴度.重组病毒感染肺腺癌细胞系NCI-H1975,通过Western-Blot实验检测siRNA效果,利用凋亡实验验证细胞生物学效应.Western-Blot实验测得siRNA有较好的干扰效果,凋亡实验测得肺腺癌细胞NCI-H1975产生了凋亡,从而证明具有感染性的靶向TTF-1的siRNA腺相关病毒载体组装成功.     

Theoretical study on stereodynamics of H + NeH+ (v = 0, j = 0) → H2 + + Ne reaction

Stereodynamics of reaction H + NeH+(v = 0,j = 0) → H2+ + Ne is investigated by quasi-classical trajectory method using a new potential energy surface constructed by Lv et al.The distributions of P(r),P(r) and PDDCSs are calculated at four different collision energies.The rotational polarization of product H2+ presents different characters at different collision energies.The product rotational angular momentum vector j’ is not only aligned,but also oriented along the direction perpendicular to the scattering plane.With the increase of collision energy,the rotation of product molecule has a preference of changing from the "in-plane" mechanism to the "out-of-plane" mechanism.Although the title reaction is mainly dominated by the direct reaction mechanism,the indirect mechanism plays a role when the collision energies are low.     

An engraved artifact from Shuidonggou, an Early Late Paleolithic Site in Northwest China

Cognition and symbolic thinking are viewed as important features of modern human behavior.Engraved objects are seen as a hallmark of cognition and symbolism,and even as evidence for language.Accumulated evidences including engraved bones,ochre,ostrich eggshells and stone artifacts were unearthed from Africa,Europe,Levant even Siberia Paleolithic sites.But the archaeological evidence for this,including beads,ornaments,burials,performed objects and engraved objects,is rarely discovered in the Pleistocene of East Asia.The present paper reports an engraved stone object unearthed in the Early Late Paleolithic levels about 30 ka BP at the Shuidonggou site(SDG) in northwestern China.It was unearthed in the 1980’s excavation from Lower culture unit of SDG1 but was identified in 2011 when the first author of this article observed the collection from the 1980’s excavations stored in the Institute of Archaeology of Ningxia Hui Autonomous Region for further detailed lithic analysis.This lithic artifact is the first engraved non-organic object of the Paleolithic period found in China.In order to clarify the details of the incisions and to document the human intentional modifications,we used a KEYENCE VHX-600 Digital Microscope to measure and observe all the incisions in 3-dimensional perspective.Comparing the natural cracks and analyzing many details of the incisions,we argue that incisions on this stone artifact are the result of intentional behaviors by ancient humans.Also,we exclude the possible other causes including animal-induced damages,post-depositional phenomenon and unintentional by-products.Combining all these features,we suggest that the incisions were made by an intentional behavior and were probably of a non-utilitarian character.Because the nature of most other engraved objects in China is debate,we cannot get a clear scenario of the emergence and progress of modern human behavior in North China.But we infer the possible existence of a counting or recording system,or other symbolic behaviors,which reflect considerably evolved cognitive capacities or modern human behavior in the Early Late Paleolithic of East Asia.     

Ionic liquids containing the urea moiety for recognition of halides and complex anions

A series of novel imidazolium based ionic liquids containing the urea moiety were designed and synthesized for anion recognition. 1-Ethylurea-3-methylimidazolium acetate ([Eumim]OAc) was used as the receptor for the halides and complex anions (BF4-, PF6-, BPh4-). 1H NMR spectra showed that the urea protons and imidazolium C(2) proton of the receptor ([Eumim]OAc) moved upfield on addition of various anions. A Job plot showed that the [Eumim]OAc receptor formed a 1:1 complex with BPh4-. X-ray diffraction analysis and the molecular modeling study revealed that the conformations of [Eumim]OAc and [Eumim]BPh4 were different. The conformational change of the cation was caused by anion exchange, and may provide an alternative to current methods for recognition of anions.     

Preparation and cellular uptake of PLGA particles loaded with lamivudine

Poly(D,L-lactide-co-glycolide) (PLGA) nanoparticles loaded with lamivudine and coated with bovine serum albumin (BSA) were prepared via a double emulsion method. The influences of experiments parameters such as volume of inner aqueous phase, concentration of organic phase and ultrasonication time on the particle size and drug entrapment efficiency were investigated, obtaining PLGA particles with a diameter of ~260 nm and drug entrapment efficiency of ~35%. The particles were observed by scanning electron microscopy and transmittance electron microscopy, showing a core-shell structure. BCA assay found that 58 mg BSA was present on/in 1 g LPB particles. The loaded lamivudine showed a burst release at beginning and sustained release until 24 h in physiological conditions. Low pH could accelerate the release of lamivudine from PLGA particles, making the PLGA particles potential intelligent intracellular drug carriers. The PLGA particles were readily internalized into the human liver cells within a short time and increased gradually with the prolongation of incubation time regardless of the loading of lamivudine. The particles either resided within lysosomes or transferred to cytoplasm, but could not enter into the cell nucleus. The cell viability was not significantly influenced in the presence of the particles regardless of lamivudine encapsulation, suggesting that this kind of particles may be a good candidate for the intracellular anti-hepatitis B drug delivery.     

Eocene high grade metamorphism and crustal anatexis in the North Himalaya Gneiss Domes,Southern Tibet

Determination of the timing and geochemical nature of early metamorphic and anatectic events in the Himalayan orogen may provide key insights into the physical and chemical behavior of lower crustal materials during the early stage of tectonic evolution in large-scale collisional belts.The Yardoi gneiss dome is the easternmost dome of the North Himalayan Gneiss Domes(NHGD),and contains three types of amphibolites with distinct mineral assemblage,elemental and radiogenic isotope geochemistry,as well as various types of gneisses.SHRIMP zircon U/Pb analyses on the garnet amphibolite and garnet-bearing biotite granitic gneiss yield ages of nearly peak metamorphism at 45.0±1.0 Ma and 47.6±1.8 Ma,respectively,which are 2 to 4 Ma older than the age for partial melting in migmatitic garnet amphibolite(43.5±1.3 Ma).Available data have demonstrated that ultra-high pressure metamorphism in the Tethyan Himalaya occurred at ～55 Ma,and high amphibolite facies to granulite facies metamorphism at 45 to 47 Ma.In addition,partial melting at thickened crustal conditions occurred at 43.5±1.3 Ma,which led to the formation of high Sr/Y ratios two-mica granites.The high-grade metamorphic rocks in the NHGD may represent the subducted front of the Indian continental lithosphere.In large collisional belts,fertile components in crustal materials could melt and form granitic melts with relatively high Na/K and Sr/Y ratios under thickened crustal conditions,significantly different from those formed by decompressional melting during rapid exhumation.     

Modern human behaviors during the late stage of the MIS3 and the broad spectrum revolution: Evidence from a Shuidonggou Late Paleolithic site

The last glacial period was vital for the distribution and evolution of early modern humans in Asia. The Shuidonggou Late Paleolithic site, dated at 30-20 ka BP, accumulated cultural remains during the important late stage of MIS 3 period in the last glacial. These remains represent characteristics of typical Late Paleolithic conditions in North China:high degree of standardization and morphological variability of tool types, exploitation of bone materials, systematic use of body decorations, extensive use of earth-pit hearths, distinct functional spatial organization within habitations, and conversion of subsistence patterns. These characteristics illustrate early modern human behaviors during the late MIS3 period, and provide clues and perspectives for the analysis of early modern human origins in China. At the same time, the conversion of subsistence patterns is considered to be a combination of multiple early modern human behaviors, as well as the result of the Broad Spectrum Revolution. In this paper, we argue for the dynamic mechanism of Broad Spectrum Revolution from a human behavioral and ecological perspective.