Heritable and inducible genetic interference by double-stranded RNA encoded by transgenes

Double-stranded RNA interference (RNAi) is an effective method for disrupting expression of specific genes in Caenorhabditis elegans and other organisms. Applications of this reverse-genetics tool, however, are somewhat restricted in nematodes because introduced dsRNA is not stably inherited. Another difficulty is that RNAi disruption of late-acting genes has been generally less consistent than that of embryonically expressed genes, perhaps because the concentration of dsRNA becomes lower as cellular division proceeds or as developmental time advances. In particular, some neuronally expressed genes appear refractory to dsRNA-mediated interference. We sought to extend the applicability of RNAi by in vivo expression of heritable inverted-repeat (IR) genes. We assayed the efficacy of in vivo-driven RNAi in three situations for which heritable, inducible RNAi would be advantageous: (i) production of large numbers of animals deficient for gene activities required for viability or reproduction; (ii) generation of large populations of phenocopy mutants for biochemical analysis; and (iii) effective gene inactivation in the nervous system. We report that heritable IR genes confer potent and specific gene inactivation for each of these applications. We suggest that a similar strategy might be used to test for dsRNA interference effects in higher organisms in which it is feasible to construct transgenic animals, but impossible to directly or transiently introduce high concentrations of dsRNA.     

立方氮化硼横向电光调制半波电压的测量

宽禁带半导体材料立方氮化硼(cBN)具有闪锌矿结构和43m点群对称性,因此cBN晶体也是电光晶体。用cBN晶体进行了横向电光调制,首次观察到cBN的电光效应,并且测得了样品的半波电压．     

计算机回归数学模型在血水草生物碱杀螺研究中的应用

通过用计算机回归数学模型对数据进行逐步回归计算和因素分类区域处理，探讨了影响血水草生物碱杀螺效果的各种因素的变化规律，发现药物浓度对灭螺效用的规律呈抛物线变化，在温度为25℃、浸泡时间为72h时达到最大；温度的效用也呈现同样的规律，在浓度为2．5ng／L、浸泡时间为72h时达到最大；而浸泡时间则不同：对于浓度的变化，它的效用呈抛物线规律，在2．5ng／L时达到最大；而对于温度的变化，则呈单调上升的趋势．根据因素分类区域分析的结果，可以得出实验室药物杀螺的最优条件．     

基于压缩感知的自适应匹配追踪算法优化

针对基于压缩感知的稀疏自适应匹配追踪(sparsity adaptive matching pursuit,SAMP)算法运行效率低的问题,给出了一种优化的自适应匹配追踪(modified adaptive matching pursuit,MAMP)算法.该算法在支撑集选择过程中对稀疏度进行了初步估计,并优化了迭代停止的条件.实验表明,该算法相比于SAMP有更快的收敛速度,并且实现更优的重建效果.     

硅氢反应合成有机硅蜡

在铂络合物催化剂的作用下,以高碳数烯烃化合物和含氢硅油为原料,利用硅氢加成反应合成有机硅蜡。研究了原料配比、催化剂用量、反应时间、反应温度对Si-H键转化率和产物运动粘度的影响。确定最佳反应条件为：烯键与硅氢健摩尔比n（C—C）：n（Si—H）-1．12：1、铂催化剂用量3．2μg／g、反应温度130℃、反应时间8h。并对合成产物的分子结构进行了红外、核磁表征,结果表明长链烷基已被接枝到聚硅氧烷主链中。     

辽宁省生物技术产业资本市场研究

资本市场是生物技术产业发展的动力和催化剂，世界生物技术产业发展强国都在很大程度上得益于其资本市场的支持，在分析生物技术产业资本市场特点的基础上，研究辽宁省生物技术产业资本市场的现状，构建辽宁省生物技术产业的资本市场体系——对处于研究开发期阶段的企业以政府资金投入为主，对处于创业期、早期成长期和加速成长期的企业以引进风险投资为主，对处于稳定成长期和成熟期的企业以银行贷款、发行债券和上市融资为主。     

Demography of the Yarrow's spiny lizard, Sceloporus jarrovii , from the central Chihuahuan Desert

The demography of a population of Yarrow's spiny lizard, Sceloporus jarrovii , was examined from 2004 to 2006 in the canyon Las Piedras Encimadas, located in Gomez Palacio, Durango, México. Lizards were studied using a mark-recapture technique. Reproduction in females occurred between November and May, coinciding with dry conditions. Reproductive activity was highest (percent of females with vitellogenic follicles or embryos) in the middle of the dry season (November and December). Thirteen percent of females reached sexual maturity at an average age of 8.5 months. The population structure was similar in spring and fall, but not in summer. A notable feature of summer, coinciding with the wet season, was the greater number of hatchlings and juveniles. The overall sex ratio did not differ from 1:1. The density of adults varied from 12 to 62 animals ? 0.5 ha –1 . Temperate and arid-adapted populations of S. jarrovii exhibited broad similarity in timing of the reproductive season, whereas factors such as density, growth, age at sexual maturity, and survivorship differed.     

两河口特大桥拱箱悬索吊装施工方法

结合工程实例,介绍拱箱悬索吊装施工方法。     

The p66shc adaptor protein controls oxidative stress response and life span in mammals

Gene mutations in invertebrates have been identified that extend life span and enhance resistance to environmental stresses such as ultraviolet light or reactive oxygen species. In mammals, the mechanisms that regulate stress response are poorly understood and no genes are known to increase individual life span. Here we report that targeted mutation of the mouse p66shc gene induces stress resistance and prolongs life span. p66shc is a splice variant of p52shc/p46shc (ref. 2), a cytoplasmic signal transducer involved in the transmission of mitogenic signals from activated receptors to Ras. We show that: (1) p66shc is serine phosphorylated upon treatment with hydrogen peroxide (H2O2) or irradiation with ultraviolet light; (2) ablation of p66shc enhances cellular resistance to apoptosis induced by H2O2 or ultraviolet light; (3) a serine-phosphorylation defective mutant of p66shc cannot restore the normal stress response in p66shc-/- cells; (4) the p53 and p21 stress response is impaired in p66shc-/- cells; (5) p66shc-/- mice have increased resistance to paraquat and a 30% increase in life span. We propose that p66shc is part of a signal transduction pathway that regulates stress apoptotic responses and life span in mammals.     

Data association based on target signal classification information

In most of the passive tracking systems, only the target kinematical information is used in the measurement-to-track association, which results in error tracking in a multitarget environment, where the targets are too close to each other. To enhance the tracking accuracy, the target signal classification information （TSCI） should be used to improve the data association. The TSCI is integrated in the data association process using the JPDA （joint probabilistic data association）. The use of the TSCI in the data association can improve discrimination by yielding a purer track and preserving continuity. To verify the validity of the application of TSCI, two simulation experiments are done on an air target-tracing problem, that is, one using the TSCI and the other not using the TSCI. The final comparison shows that the use of the TSCI can effectively improve tracking accuracy.