核酸分析中的新突破双-嵌入剂荧光探针

近年来兴起的双－嵌入剂是一类安全、方便、高灵敏度、低背景的理想核酸探针,当使用激光激发的共焦荧光扫描仪检测时,对核酸的分析可达ｐｇ级,赶上甚至超过了放射性同位素标记物．本文对双－嵌入剂荧光核酸探针的结构、性质、应用及发展展望几方面进行了综述．     

多孔介质应力关系方程

Ｔｅｒｚａｇｈｉ方程（σ＝σＧ＋Ｐ）是描述多孔介质应力关系的经典方程,由于方程同时定义了二个不可测量,因而是不实用的;方程没有引入反映多孔介质特性的最重要参数（φ）,因而是不妥当的;鉴于方程存在的问题,本文对其进行了深入的研究,重新建立了多孔介质应力之间的关系方程：σ＝φＰ＋（１－φ）σＧ;由于φ的引入,新方程更全面、更合理地反映了多孔介质固有的力学特性．     

泥沙输移对长江中游水位抬升的影响

通过对长江干流宜昌至汉口河段及与洞庭湖区泥沙输移变化的综合分析,得到了三口分流分沙逐年减少是造成洞庭湖区淤积速率减小和螺山至汉口河段淤积速率增大的主要原因,也是造成长江干流监利至螺山河段水位逐年抬升、洞庭湖区的洪水调节能力大幅度下降的主要原因等结论．按照因势利导的治理原则,荆江及洞庭湖的近期治理策略应是淤积围垸减少河道淤积、疏浚河道、加固干堤．     

一种新的混沌神经网络模型及其动力学分析

提出了一种混沌神经网络模型。通过引入暂态混沌和时变增益,该网络比Hopfield型网络具有更加丰富和更为灵活的动力学特性,从而具有更强的搜索全局最优解或近似全局最优解的能力,它可以用于求解各种复杂的优化问题。大量的数字模拟表明网络能较好地解决Hopfield型网络的局部极值问题。     

AHP法在空调冷热源方案选择中的应用

从空调冷热源设计的实际需要出发,提出了将系统工程中著名的ＡＨＰ法引入设计方案决策的新途径．在简要论述ＡＨＰ法基本思想和步骤的基础上,着重分析了ＡＨＰ法在实用中存在的问题,探讨了一种直观、高效的构造具有足够满意一致性ＡＨＰ判断矩阵的新方法,并运用该法对某大厦空调冷热源设计的具体实例进行了计算,得出了合理的结论．结果表明,ＡＨＰ法能够成功地应用于空调冷热源方案选择的决策．     

基于领域复用的软插件库应用研究

目的研究基于领域复用的软插件的原理和实现模型。方法 结合基于领域复用的基本原则,对构造基于领域复用的软插件库的方法进行讨论,并以商用ＰＯＳ机软插件库有组织构造为例进行了具体说明。结果与结论基于领域复用的软插件比传统方法更有利于软件复用。     

基于任务的产品工艺设计过程管理

在分析产品工艺设计流程现状的基础上,研究了现有ＣＡＰＰ系统中工艺过程设计和过程管理,提出了任务驱动型的ＣＡＰＰ思想．认为工艺设计的过程本质上是对任务完成过程的集合,通过对任务的生成、监控、设计、提交、签审、发放,正好控制工艺设计的流程,并在ＣＡＰＰ系统开发中进行了成功的尝试,为工艺设计的管理提供了一种可行的方法．     

Preparation of enantiomerically pure 1,1′-bi-2-naphthols from 85% cinchonine and 1,1′-bi-2-naphtholboric anhydride

A possibility of preparation of enantiomerically pure 1,1′-bi-2-naphthols using an impure cinchonine has been examined. The solid and the mother liquor formed from the reaction of 1,1′-bi-2-naphtholboric anhydride and 85% cinchonine in toluene could give optically pure (S)-(−)- and (R)-(+)-1,1′-bi-2-naphthol after acidification and kinetic crystallization, the overall yields were 40% and 28%, respectively. Supported by the National Natural Science Foundation of China (2937206) and the Natural Science Foundation of Hubei Province (93J27) Xiong Ying, born in 1971, Graduate student     

Spontaneous transformation of C60Br24 to C60 in solvents

It was found that C₆₀Br₂₄ transforms to C₆₀ spontaneously in some solvents. The intermediate, C₆₀Br₈, can be separated in some cases. When the bromine trap, phenol, was added,p-bromophenol and other brominated product were obtained and identified. The influences of time, the kind of solvent, etc were investigated. Possible mechanism was suggested. Supported by the National Natural Science Foundation of China Liu Ying: born in 1969, Ph. D     

Cloning and expression of the vp39 gene ofBombyx mori nuclear polyhedrosis virus inE. coli

The nuclear capsid protein gene (vp39) ofBombyx mori nuclear polyhedrosis virus (BmNPV) was amplified successfully by PCR technique and inserted into pGEM 3zf(+). The 5′ and 3′ terminal area of the amplified vp39 gene were sequenced with silver-staining dideoxy method. Bmvp39 gene was sub-cloned into the expression vector pRSET-A, and transformed intoE. coli BL21. This gene was highly expressed by IPTG induction. SDS-PAGE analysis showed that the expressed protein is about 38 kd, and the expressed amount reached maxium in 4 h with IPTG induction. Supported by the National Natural science Foundation of China and the Doctoral Foundation of Edn carto Committee Liu Deli: born in 1954, Doctoral Candidate from Huazhong Normal University To whom correspondence should be addressed: (027-7882712-2938)