Effect of platelet membranes on the electrophoretic pattern of 6-phosphogluconic dehydrogenase from platelet lysates

Riassunto L'incubazione di lisati piastrinici con NADP provoca inattivazione della 6PGD e ne modifica la migrazione elettroforetica. Non si osserva invece attivazione della GSSG-R.

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Supported by a grant from the Consiglio Nazionale delle Ricerche Contratto No 70.00378/18/115.3565. Roma (Italy).     

Fluorogenic amines in the notochords of chick embryos treated by cholinergics]

The investigation of 3 day old notochords by the fluorescence method of Falck and Owman showed an increase of the fluorescence after treatment of the chick embryos at 48 hrs of incubation, with nicotine sulfate or carbachol. The addition of norepinephrine or L-Dopa emphasized the formaldehyde induced fluorescence. The simultaneous treatment with a cholinergic agent and atropine, propranolol or reserpine decreased or suppressed the fluorescence. These results demonstrate the existence of a relationship between the treatment with a cholinergic agent and the amount of chordal biogenic amines.