Expression of lysine-rich protein gene and analysis of lysine content in transgenic wheat

Expression vector pBPC102, which carries winged bean lysine-rich protein (wblrp) gene and dihydropicolinate synthase (DHDPS) gene, was transferred into hexaploid winter wheat cv. Jinghua No.l, Jing411, You899 and Yangnongl5 explants of immature inflorescence and immature embryos by particle bombardment. More than 100 transgenic plants were obtained under the selection of s-(2-aminoethyl)-L-cysteine (AEC). Confirmed transgenic plants of To and TI generation by PCR and PCR-Southern blotting analyses showed successful integration of wblrp gene into wheat genome. Analysis of transgenic plant lines of T2 by Northern dot-blotting showed good expression of wblrp gene in offspring seed. The content of free lysine in leaves, contents of bound lysine and total proteins in seeds of T2 transgenie wheat lines were determined and analyzed. Among 34 tested transgenic lines, levels of free lysine content in leaves of 9 transgenic lines are 2~3times higher than un-trans-formed wild-type cultivars. Among 17 analyzed transgenic lines, bound lysine content of 4 transgenic lines is more than 10% higher than that of wild-type cultivars. Our research suggests that introducing wblrp gene into wheat is an effective way to improve its nutrition quality.     

An early cretaceous<Emphasis Type="Italic">Ginkgo</Emphasis> ovule-bearing organ fossil from Liaoning,Northeast China and its evolutionary implications

Ginkgo is a very important gymnospermous genus that has only one surviving species, Ginkgo biloba L. As a so-called “living fossil“, wild Ginkgo was found only in China, but it once flourished well and had a world-wide distribution in the Mesozoic. The earliest Ginkgo has been known from the Middle Jurassic, based on ovule-bearin gorgans. In the Jurassic and Early Cretaceous, Ginkgo was one of the dominant gymnosperms in floras and possibly one of the coal-forming plants in North China. During the Early Tertiary, it reduced to a circumpolar distribution in the North Hemisphere; only a few reported from the South Hemisphere. From the Pliocene onwards, the Ginkgoales has been represented by the extant monotypic “living fossil“ Ginkgo biloba.     

Characterization of MASDs of surface soils in north China and its influence on estimating dust emission

Inordertoquantitivelyevaluateeffectsofdustaerosolonradiativeforcing[1],andtounderstandtheprocessofatmosphericchemistryandbiogeochemistry[2,3],modelingthedustemissionfluxisakindoffundamentalwork.TheDPMmodel[4—6]isidealforpredictingdustemission[7—10]becauseitconsiderseffectsofsurfacefea-tures(MASDofsurfacesoil,roughnesslengthandsoilmoisture,etc)andsandblastingprocess.Italsoprovidesanexplicitphysicalmechanismthatisvalidatedbywindtunnelandfieldexperiments[7—10].Themeandiameterofsurfacesoilsi…     

Transformation of the salt-tolerant gene of<Emphasis Type="Italic">Avicennia marina</Emphasis> into tobacco plants and cultivation of salt-tolerant lines

Salt-tolerant gene, CSRG1, which was isolated from a kind of salt-tolerant mangroves, Avicennia marina, constructed the transgenic plasmid, pGAM189/CSRG1. CSRG1, GUS, Kmr and Hyg^r could be transferred into tobacco genome by the ameliorated leaf discs method of agro-bacterium-mediate transformation. Thirteen stable resistant lines were obtained when fifty transgenic explants were selected through 50 mg/L hygromycin and 150 mg/L kanamycin. Assessments of PCR amplification, Southern blot analysis and GUS histochemical staining showed that CSRG1 has been integrated into the genome of the eleven transgenic lines (frequency of transformation was 22%). Northern bolt analysis revealed that CSRG1 had expressed in transgenic lines. The assessments of salt-tolerant ability and photosyn-thetic rates indicated that the survival rate of the transgenic lines is 80%—90% and the transgenic lines could increase by 30%—40% in plant height, even when they were cultivated in MS medium containing 2% NaCl and the total seawater (salinity 24). It is supposed that the special physiologic metabolic pathway formed by the products of CSRG1 can really endow the tobacco plants with the high salt-tolerant ability, not only to Na^ stress, but also to the comprehensive stress of various ions.     

Anion-exchangeable inorganic-organic hybrid materials synthesized without using templates

Inorganic-organic hybrid materials have been obtained at room temperature in aqueous solution without using the templates of surfactants. The materials are carefully characterized by anion-exchange measurement, elements analysis, X-ray diffraction, and infrared spectroscopy. Notably, the anion-exchange capacity of the samples (3.9mmol.g^-1) is even higher than anion-exchangeable resins. Interestingly, both small and large anions could be easily exchanged into the samples due to the plasticity of the samples, along with the phase transition.     

Second generation YBCO coated conductors： A review

The advance in first generation Bi-2223 HTS wire has enabled the demonstration of superconducting power cables, magnetic energy-storage devices, transformers,fault current limiters and motors. However, the low irreversibility field (H^*) prevents application of Bi-2223 at 77 Kin any significant field. Worldwide activities are therefore,focused on developing a second-generation HTS technology based on YBCO, for which H^* (77K)-7 T. In this paper, we discuss the status and commercial prospects of second generation HTS wire technologies. In addition, we review the recent results and discuss the prospects of future applications.     

Miniaturized volume holographic optical data storage and correlation system with a storage density of 10 Gb／cm^3

The general idea of holographic optical data storage (HODS) is briefly introduced. Based on the recent advances of HODS, the key techniques and the challenges of HODS are discussed. Some new techniques are proposed to improve the system. A miniaturized volume holographic data storage and correlation system is presented. It can achieve a density of 10 Gb/cm^3 and a fast correlation recognition rate of more than 2000 images per second. It shows the attracting potential advantages over other conventional storage methods in the information storage as well as information processing.     

Infectious foot-and-mouth disease virus derived from a cloned full-length cDNA of OH／CHA／99

The China foot-and-mouth virus (FMDV) isolate OH/CHA/99 was isolated from swine, which was unable to infect bovine thyroid cells in vitro or to cause typical disease in bovines following intradermal inoculation in the tongue. To enhance antigenicity, replication, maturation and pathogenicity studies of OH/CHA/99, an infectious fulllength cDNA clone, designated pBIFMDV, was prepared. The in vitro and in vivo biological properties of the virus derived from pBIFMDV were studied by analyzing antigenicity, plaque morphology and virulence in pigs. The results showed that the virus derived from pBIFMDV had the same biological properties as the parent strain OH/CHA/99; the fulllength infections cDNA clone, pBIFMDV, will be very useful in studies of the antigenicity, virulence, pathogenesis, maturation and replication of FMDV.     

A new model for analyzing laminar forced convective enhanced heat transfer in latent functionally thermal fluid

In this paper, a new model to analyze laminar forced convective enhanced heat transfer in latent functionally thermal fluid is developed. The main characteristics of the model are- i) a new formula of the specific heat at constant pressure is used; ii ) a real heat transfer process is considered; that is, heat transfer processes occur not only between working fluid and microcapsules, but also between the mixture and tube wall; iii) the new method, which combines the newly developed axisymmetrical dual reciprocity boundary element method (DRBEM) with finite difference method (FDM), is used to solve the control equations of this problem. The new model is validated by experimental data. Some new physical results on the variational characteristics of the specific heat at constant pressure with space and time during phase-change process, the time-marching history of the phase-change interfaces and so on are obtained. Several main physical factors that affect enhanced heat transfer in latent functionally thermal fluid are numerically analyzed. Some new understandings for the mechanism of enhanced heat transfer in the functionally fluid are obtained.