全文获取类型
收费全文 | 11341篇 |
免费 | 100篇 |
国内免费 | 166篇 |
专业分类
系统科学 | 195篇 |
丛书文集 | 609篇 |
教育与普及 | 458篇 |
理论与方法论 | 35篇 |
现状及发展 | 1038篇 |
研究方法 | 1377篇 |
综合类 | 7879篇 |
自然研究 | 16篇 |
出版年
2018年 | 21篇 |
2017年 | 34篇 |
2016年 | 21篇 |
2015年 | 28篇 |
2014年 | 56篇 |
2013年 | 46篇 |
2012年 | 680篇 |
2011年 | 870篇 |
2010年 | 234篇 |
2009年 | 115篇 |
2008年 | 832篇 |
2007年 | 867篇 |
2006年 | 915篇 |
2005年 | 971篇 |
2004年 | 740篇 |
2003年 | 701篇 |
2002年 | 635篇 |
2001年 | 498篇 |
2000年 | 722篇 |
1999年 | 241篇 |
1998年 | 62篇 |
1997年 | 47篇 |
1996年 | 33篇 |
1995年 | 33篇 |
1994年 | 41篇 |
1993年 | 38篇 |
1992年 | 29篇 |
1991年 | 43篇 |
1990年 | 51篇 |
1989年 | 54篇 |
1988年 | 41篇 |
1987年 | 40篇 |
1986年 | 61篇 |
1985年 | 52篇 |
1984年 | 38篇 |
1983年 | 34篇 |
1982年 | 52篇 |
1981年 | 48篇 |
1980年 | 25篇 |
1979年 | 28篇 |
1971年 | 34篇 |
1970年 | 61篇 |
1966年 | 29篇 |
1959年 | 169篇 |
1958年 | 290篇 |
1957年 | 203篇 |
1956年 | 176篇 |
1955年 | 197篇 |
1954年 | 191篇 |
1948年 | 53篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
681.
De-ubiquitination and ubiquitin ligase domains of A20 downregulate NF-kappaB signalling 总被引:1,自引:0,他引:1
Wertz IE O'Rourke KM Zhou H Eby M Aravind L Seshagiri S Wu P Wiesmann C Baker R Boone DL Ma A Koonin EV Dixit VM 《Nature》2004,430(7000):694-699
682.
The homeotic protein AGAMOUS controls microsporogenesis by regulation of SPOROCYTELESS 总被引:1,自引:0,他引:1
Ito T Wellmer F Yu H Das P Ito N Alves-Ferreira M Riechmann JL Meyerowitz EM 《Nature》2004,430(6997):356-360
683.
Our knowledge of the eggs and embryos of pterosaurs, the Mesozoic flying reptiles, is sparse. Until now, the recent discovery of an ornithocheirid embryo from 121-million-year-old rocks in China constituted the only reliable evidence of an unhatched pterosaur. Here we describe an embryonic fossil of a different pterosaur from the Early Cretaceous lacustrine deposits of Loma del Pterodaustro (the Lagarcito Formation, which is about 100 million years old) in central Argentina. This new fossil provides insight into the eggshell morphology, early growth and nesting environments of pterosaurs. 相似文献
684.
685.
686.
Worobey M Santiago ML Keele BF Ndjango JB Joy JB Labama BL Dhed'A BD Rambaut A Sharp PM Shaw GM Hahn BH 《Nature》2004,428(6985):820
Despite strong evidence to the contrary, speculation continues that the AIDS virus, human immunodeficiency virus type 1 (HIV-1), may have crossed into humans as a result of contamination of the oral polio vaccine (OPV). This 'OPV/AIDS theory' claims that chimpanzees from the vicinity of Stanleyville--now Kisangani in the Democratic Republic of Congo--were the source of a simian immunodeficiency virus (SIVcpz) that was transmitted to humans when chimpanzee tissues were allegedly used in the preparation of OPV. Here we show that SIVcpz is indeed endemic in wild chimpanzees of this region but that the circulating virus is phylogenetically distinct from all strains of HIV-1, providing direct evidence that these chimpanzees were not the source of the human AIDS pandemic. 相似文献
687.
Mechanisms of gene silencing by double-stranded RNA 总被引:6,自引:0,他引:6
Double-stranded RNA (dsRNA) is an important regulator of gene expression in many eukaryotes. It triggers different types of gene silencing that are collectively referred to as RNA silencing or RNA interference. A key step in known silencing pathways is the processing of dsRNAs into short RNA duplexes of characteristic size and structure. These short dsRNAs guide RNA silencing by specific and distinct mechanisms. Many components of the RNA silencing machinery still need to be identified and characterized, but a more complete understanding of the process is imminent. 相似文献
688.
Adhesive type 1 pili from uropathogenic Escherichia coli strains have a crucial role during infection by mediating the attachment to and potentially the invasion of host tissue. These filamentous, highly oligomeric protein complexes are assembled by the 'chaperone-usher' pathway, in which the individual pilus subunits fold in the bacterial periplasm and form stoichiometric complexes with a periplasmic chaperone molecule that is essential for pilus assembly. The chaperone subsequently delivers the subunits to an assembly platform (usher) in the outer membrane, which mediates subunit assembly and translocation to the cell surface. Here we show that the periplasmic type 1 pilus chaperone FimC binds non-native pilus subunits and accelerates folding of the subunit FimG by 100-fold. Moreover, we find that the FimC-FimG complex is formed quantitatively and very rapidly when folding of FimG is initiated in the presence of both FimC and the assembly-competent subunit FimF, even though the FimC-FimG complex is thermodynamically less stable than the FimF-FimG complex. FimC thus represents a previously unknown type of protein-folding catalyst, and simultaneously acts as a kinetic trap preventing spontaneous subunit assembly in the periplasm. 相似文献
689.
Stomp M Huisman J De Jongh F Veraart AJ Gerla D Rijkeboer M Ibelings BW Wollenzien UI Stal LJ 《Nature》2004,432(7013):104-107
The dazzling diversity of the phytoplankton has puzzled biologists for decades. The puzzle has been enlarged rather than solved by the progressive discovery of new phototrophic microorganisms in the oceans, including picocyanobacteria, pico-eukaryotes, and bacteriochlorophyll-based and rhodopsin-based phototrophic bacteria. Physiological and genomic studies suggest that natural selection promotes niche differentiation among these phototrophic microorganisms, particularly with respect to their photosynthetic characteristics. We have analysed competition for light between two closely related picocyanobacteria of the Synechococcus group that we isolated from the Baltic Sea. One of these two has a red colour because it contains the pigment phycoerythrin, whereas the other is blue-green because it contains high contents of the pigment phycocyanin. Here we report theory and competition experiments that reveal stable coexistence of the two picocyanobacteria, owing to partitioning of the light spectrum. Further competition experiments with a third marine cyanobacterium, capable of adapting its pigment composition, show that this species persists by investing in the pigment that absorbs the colour not used by its competitors. These results demonstrate the adaptive significance of divergence in pigment composition of phototrophic microorganisms, which allows an efficient utilization of light energy and favours species coexistence. 相似文献
690.
A dialogue between the hippocampus and the neocortex is thought to underlie the formation, consolidation and retrieval of episodic memories, although the nature of this cortico-hippocampal communication is poorly understood. Using selective electrolytic lesions in rats, here we examined the role of the direct entorhinal projection (temporoammonic, TA) to the hippocampal area CA1 in short-term (24 hours) and long-term (four weeks) spatial memory in the Morris water maze. When short-term memory was examined, both sham- and TA-lesioned animals showed a significant preference for the target quadrant. When re-tested four weeks later, sham-lesioned animals exhibited long-term memory; in contrast, the TA-lesioned animals no longer showed target quadrant preference. Many long-lasting memories require a process called consolidation, which involves the exchange of information between the cortex and hippocampus. The disruption of long-term memory by the TA lesion could reflect a requirement for TA input during either the acquisition or consolidation of long-term memory. To distinguish between these possibilities, we trained animals, verified their spatial memory 24 hours later, and then subjected trained animals to TA lesions. TA-lesioned animals still exhibited a deficit in long-term memory, indicating a disruption of consolidation. Animals in which the TA lesion was delayed by three weeks, however, showed a significant preference for the target quadrant, indicating that the memory had already been adequately consolidated at the time of the delayed lesion. These results indicate that, after learning, ongoing cortical input conveyed by the TA path is required to consolidate long-term spatial memory. 相似文献