<Emphasis Type="Italic">Yersinia pseudotuberculosis</Emphasis> supports Th17 differentiation and limits de novo regulatory T cell induction by directly interfering with T cell receptor signaling

Adaptive immunity critically contributes to control acute infection with enteropathogenic Yersinia pseudotuberculosis; however, the role of CD4⁺ T cell subsets in establishing infection and allowing pathogen persistence remains elusive. Here, we assessed the modulatory capacity of Y. pseudotuberculosis on CD4⁺ T cell differentiation. Using in vivo assays, we report that infection with Y. pseudotuberculosis resulted in enhanced priming of IL-17-producing T cells (Th17 cells), whereas induction of Foxp3⁺ regulatory T cells (Tregs) was severely disrupted in gut-draining mesenteric lymph nodes (mLNs), in line with altered frequencies of tolerogenic and proinflammatory dendritic cell (DC) subsets within mLNs. Additionally, by using a DC-free in vitro system, we could demonstrate that Y. pseudotuberculosis can directly modulate T cell receptor (TCR) downstream signaling within naïve CD4⁺ T cells and Tregs via injection of effector molecules through the type III secretion system, thereby affecting their functional properties. Importantly, modulation of naïve CD4⁺ T cells by Y. pseudotuberculosis resulted in an enhanced Th17 differentiation and decreased induction of Foxp3⁺ Tregs in vitro. These findings shed light to the adjustment of the Th17-Treg axis in response to acute Y. pseudotuberculosis infection and highlight the direct modulation of CD4⁺ T cell subsets by altering their TCR downstream signaling.     

未知噪声统计的交错估计GPS导航定位算法

将未知噪声统计量的估计与信号状态估计交替进行的算法引入 Ｇ Ｐ Ｓ定位,计算机模拟结果表明效果很好。     

浅谈盾构隧道推进及衬砌拼装

文章针对在松软含水地层中采用手掘式敞胸盾构施工,需辅以气压、人工井点降水及其他地层加固措施,论述了在这种条件下盾构开挖掘进时的几种施工方法.     

高动态GPS/INS组合导航算法研究

首先介绍了新型深组合GPS/INS系统原理图及组合导航滤波算法。在该算法中,组合卡尔曼滤波器除完成INS误差及GPS接收机时钟误差的估计外,还参与了GPS码跟踪,即完成传统码跟踪环中环路滤波器的功能。采用自适应码跟踪误差估计器补偿组合卡尔曼滤波器测量值中的相关分量,从而消除了传统组合中不稳定的主要根源。然后进行了计算机仿真计算,仿真结果表明,新型深组合GPS/INS导航算法适用于机动性较高的载体。     

汽车电子机械制动器的效能分析

为了分析电子机械制动器（electmmechanical bloking,简称EMB）的制动效能,建立了电子机械制动器的数学模型和在水平路面上汽车制动时整车动力学模型,运用MATLAB／SIMULINK仿真软件对电子机械制动器和液压制动器（hydmulic braking简称HB）的制动性能进行了比较．结果表明：电子机械制动器的制动效能明显优于传统的液压制动器,为电子机械制动器工程开发提供了理论依据.     

浅谈室内绿化设计的原则与作用

随着生产力的高度发展和生活水平的日益提高,人类对绿色自然的需求也更为强烈,以至于绿色植物已发展成为室内设计中重要的一部分.在现代室内设计中,节能环保的设计理念已成为主流,合理利用自然资源,减少能源的消耗,关注健康,回归自然,扩展了室内设计的内涵,使室内设计走向更高层次和境界.     

高职院校困难学生心理危机干预的策略研究

高职学生安全管理体系是一个复杂系统,困难学生心理危机干预体系是高职学生安全管理系统的内容之一。困难学生群体因家庭困难、自尊心强、心理负担重、易受外界伤害等特点自身易诱发心理危机。学校应构建困难学生心理危机干预体系,当心理危机出现时,学生安全管理系统应给予及时、有效、准确的干预和帮扶。     

Optical detection of acetylcholine esterase based on CdTe quantum dots

In this study, we have constructed a simple, sensitive and rapid biosensor for detection of acetylcholine esterase (AChE) based on CdTe quantum dots (QDs). The detection limit of AChE by one-step enzyme reaction based on the thiolglycolic acid (TGA) stabilized QDs (TGA-QDs) was 10 U/L and the linear range was 10-100 and 100-1200 U/L, respectively. The detection limit of AChE by two-step enzyme reaction based on the 3-mercaptopropionic acid (MGA) stabilized QDs (MGA-QDs) was found to be 20 U/L and the linear range was 100-2500 U/L. The experimental conditions of biosensors were optimized, and the detection mechanism was studied. We also detected AChE in serum samples based on TGA-QDs or MGA-QDs. The linear range was 10-140 and 50-1000 U/L, respectively. The excellent performance of this novel biosensor demonstrated that this strategy has prodigious potential to be applied in practice detection of AChE.