Archaeal acylamino acid releasing enzyme/lipase:Crystallization and preliminary crystallographic analysis in a new crystal form

A primitive orthorhombic crystal form of acylamino acid releasing enzyme‘lipase(APE1547)from hyperthermophilic archaeon Aeropyrum pernix strain K1 has been obtained at 291K.The diffraction pattern of the crystal extends to 0.27nm resolution at 100K using Cu Kαradiation.The crystal belongs to the space group P212121 with unit cell dimensions of b=6.399,b=10.439and c=16.953nm.The presence of two molecules per asymmetric unit gives a crystal volume per protein mass(Vm)of 0.0022nm^3 Da^-1 and a solvent content of 43% by volume.A full set of X-ray diffraction data were collected to 0.3nm from the native crystal.     

^210Pbex depth distribution in soil and calibration models for assessment of soil erosion rates from ^210Pbex measurements

Throughout the past decade, the potential for using nuclear weapon-produced 137Cs ( half-life 30.2 a) fallout to quantify soil erosion rates on both cultivated and uncul- tivated lands for medium-term timescales (≈ 40 a) has been successfully demonstrated in a wide range of envi- ronments in different regions of the world[1—6]. Because in difficulties of distinguishing Chernobyl-derived compo- nent from total 137Cs input in many parts of Europe, and in measuring low activity samples of…     

A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)

Severe Acute Respiratory Syndrome (SARS) is a newly identified infectious disease[1—5]. The global outbreak of SARS has been threatening the health of people worldwide and has killed 353 people and infected more than 5462 in 27 countries, as reported by WHO on April 29, 2003 (http://www.who.int/csr/sarscountry/en). Although it has been recognized that a variant of virus from the family of coronavirus might be the candidate pathogen of SARS[1—5], its identity as the unique pathogen sti…     

东营凹陷胜坨地区沙三-沙四段扇体特征与岩性油气藏

东营凹陷胜坨地区沙四段、沙三段沉积时期 ,发育了洪积扇、近岸水下扇和浊积扇 3种扇体沉积 ,根据其沉积、成藏特征分析了 3种扇体的成藏规律和分布特点。研究结果表明 ,沙四段沉积期主要发育洪积扇 ,岩性以砾岩、含砾砂岩为主 ,断层附近砂体的储集性能好 ,多形成背斜和断块油气藏 ;沙三段下部沉积期发育近岸水下扇 ,岩性以砾岩、含砾砂岩为主 ,扇中储集物性最好 ,多形成构造岩性油气藏 ;沙三段中部沉积期发育浊积扇 ,岩性以砂岩为主 ,储集性能好 ,但砂体薄 ,多形成透镜体和上倾尖灭油气藏     

舟桥丙丁接头接触分析的计算机模拟

深入考察接触行为的特点，并以舟桥纵向连接件丙丁接头为例，建立了两种接触计算模型。采用大型通用有限元程序进行分析，模拟丙丁接头受力时的应力应变。与经验合成计算结果和常规有限元分析的结果进行对比，证明接触分析方法对连接件塑性变形、疲劳分析和优化设计有重要的指导意义。     

[(n-C4H9)2Sn(2-PyCO2)2(H2O)] 的合成、晶体结构

利用三丁基氯化锡和2-吡啶甲酸在三乙胺存在下以1:1摩尔比反应,合成了非预期有机锡配合物[(n-C4H9)2Sn(2-PyCO2)2(H2O)].通过元素分析、红外光谱、核磁共振氢谱和X-射线单晶衍射对其结构进行了表征.测试结果表明,该配合物为单体结构,锡原子呈七配位畸变五角双锥构型.通过配位水分子和游离羰基氧原子的氢键作用,形成了二维网状结构.     

高科技企业竞争的网络特征及其对策

本文从提高我国高科技企业持续创新能力的现实需要出发 ,在对高科技产业核心资源和市场特性分析的基础上 ,从网络效应角度具体分析了其竞争的网络特征 ,最后从培育核心能力 ,实施网络作战等方面提出了在网络竞争环境下 ,提升高科技企业竞争优势的具体对策。     

Evolution of the South China Sea and monsoon history revealed in deep-sea records

As the third summary report of ODP Leg 184 to the South China Sea (SCS), this paper discusses the evolution of the East Asian monsoon and the SCS basin. A multi-proxy approach, involving geochemistry, micropale-ontology, pollen and other analyses, was adopted for reconstructing the evolutionary history of the East Asian monsoon, which was characterized by a series of paleo-climate events especially at 8, 3.2, 2.2 and 0.4 Ma. The new record indicates similar stages in the development of the East and South Asian monsoons, with an enhanced winter monsoon over East Asia being the major difference. The rich spectrums of monsoon variability from the southern SCS also reveal other characteristic features of the low latitude ocean. Evidence for the evolution of the SCS includes the hemipelagic Oligocene sediments, implying the existence of deep water environments during the early seafloor spreading stage of the SCS basin. The four major unconformities and some remarkabl ediagenetic features in upper Oligocene deposits indicate the strongest tectonic events in the region. From a careful comparison of lithologies and sedimentation rates, we conclude that the prominent differences in sedimentary environments between the southern and northern SCS were established only by ～3 Ma.     

非线性尘埃声波在含有2种不同温度离子的尘埃等离子体中的稳定性问题

在无磁场的尘埃等离子体中 ,由冷的尘埃颗粒和热的电子和离子组成的尘埃等离子体 ,对于有限的小振幅的非线性波的运动可以用KdV方程描述 .对由 2种不同温度离子的尘埃等离子体中的弱二维尘埃声波 ,得到了它所满足的KP方程 .并且发现 ,在横向扰动下 ,弱的非线性孤立波在尘埃等离子体中是稳定的 .在这个系统中既存在压缩孤立波又存在稀疏孤立波     

小波变换在检测粘接件信号特征中的应用

在对金属与非金属粘接件性能检测的分析处理中，应用小波变换的方法对这种非平稳过程的信号进行分析，有效提取了性能检测所需要的特征参数，为提高金属与非金属粘接件性能检测的准确率和效率奠定了基础。