Rational design of glycerol dehydratase： Swapping the genes encoding the subunits of glycerol dehydratase to improve enzymatic properties

1,3-propanediol (1,3-PD) is an important material for chemical industry,and there has been always much interest in the production of 1,3-PD using all possible routes. The genes encoding glyc-erol dehydratase (GDHt) from Citrobacter freundii,Klebsiella pneumoniae and metagenome were cloned and expressed in E. coli. All glycerol dehy-dratases but the one from metagenome could be detected to show enzyme activities. In order to im-prove the enzymatic properties of GDHts,the genes encoding α and β-γ subunits were cloned,and the enzyme characteristics were evolved by rational de-sign based on their 3D structures which were con-structed by homology modeling. Six heteroenzymes were obtained by swapping the α subunit genes of these three different-source-derived GDHts. The pH,thermal stability and Vmax of some heteroenzymes were dramatically improved by 2―5 times compared with the wild one (GDHtKP). The GDHt cloned from metagenome,originally proved to be with no enzyme activity,was converted into active enzyme by swap-ping its subunits with other different GDHts. In addi-tion,the effect of subtle 3D structural changes on the properties of the enzyme was also observed.     

Ability to detect weak effective seismic signals by utilizing chaotic vibrator system

Taking the advantage of CVS (Chaotic Vibrator System) sensitivity of large-scale periodic phase-state response to quasi-periodic or periodic signals,a series of numerical experiments were made to understand the ability of CVS to detect weak effective seismic signals in the common-shot seismic record distorted by strong stochastic noise. The re-sults demonstrate that the large-scale periodic phase-states of CVS are correlated with the signal composition of the quasi-periodic wavelet sequence constructing from horizontal moveout of seismic events,noise strength and the noise distortion de-gree to signal. For the same kind of events,the higher the noise distortion degree is,the lower the detectable SNR can be reached by CVS. For seismic data with the same noise distortion degree,the closer the scanning seismic velocity (the trial moveout ve-locity) approaches to the accurate velocity,the higher the detectable SNR can be reached by CVS. More-over,the truncating scanning velocities form an asymmetric belt,which indirectly makes CVS achieve a large-scale periodic phase-state and then the ratio of wavelet distortion coefficients in events can be a biggish variable scope.     

Application of Nanometer-Size Titanium Dioxide in Extreme-Trace V（Ⅴ） Analysis

0Introduction Vanadiumplaysanimportantroleinmodernindustry,es peciallyinsteelandchemicalindustry.Forinstance,itscompoundsarewidelyappliedintheproceduresofvitrioland petroleumchemicalmanufactureascatalyzers[14].Vanadium hasseveralvalences,butgenerallyitslowvalencesturnintohighoneseasilyinenvironment[5].BecauseV(Ⅴ)isthemost stableandpoisonousone,weoftenlayemphasisonitinenvi ronmentalpollutioncontrol.Vanadiumexistsinenvironmentalwaterwithextremelylowconcentration.Inseawateritscontentislessthan…     

二亚苄基山梨糖醇系列产品的合成

研究了合成二亚苄基山梨糖醇(DBS)、二(对甲基苄叉)山梨糖醇(p-CH3-DBS)和二(对氯苄叉)山梨糖醇(p-Cl-DBS)系列产品的投料摩尔比、反应用溶剂、催化剂对甲基苯磺酸和搅拌速度等因素对DBS产品收率和熔点的影响.在苯甲醛(或对甲基苯甲醛、对氯苯甲醛)和山梨糖醇投料摩尔比为(2.0～2.1):1,对甲基苯磺酸、环己烷用量分别为苯甲醛和山梨糖醇投料总质量的4.0%和550%,反应时间6 h条件下,DBS、p-CH3-DBS和p-Cl-DBS产品收率大于70%,熔点分别为210～216 ℃、230～240 ℃和245～260 ℃.     

高校固定资产网络管理系统的研究与设计

通过对高校校园网的安全性、管理系统结构及固定资产网络管理系统功能的分析,本文介绍了高校固定资产网络管理系统的设计方案.同时,对校园网构建网络安全体系,保障固定资产网络管理系统的安全运行,提出了相应的解决方案.     

昆虫内外激素的应用前景

农药一般具有毒性,其负面影响往往相当严重.科学家们对于虫害治理的理念开始由“灭杀”向“凋控”转变.昆虫内外激素可以用于调控昆虫的行为,这类物质的研究成为人们关注的热点课题.     

发现对象技术在高校设备管理系统开发中的应用

介绍了一种利用FPGA代替单片机与计算机或监测板直接通信,实现本地与远端设备工作状态监测的方法。多台设备可通过RS485总线级联,从而实现整个网络的监测。该功能模块与其它功能模块一起在XILINX公司的单片FPGA上实现。     

初中数学学习中的生成性知识初探

以2004年为基准年,2010年为预测年,利用CALPUFF空气质量模型,研究了贵州“西电东送”火电项目火电厂排放导致的NO2时空分布特征,及对各关心点NO2的贡献浓度。结果表明:现有电厂排放主要对贵州本省中、西部地区的NO2有一定影响,对重庆市略有影响。规划电厂投产后,NOx排放量增多,对区域环境NO2的贡献值有所增加。另外,由于一些电厂靠近环境敏感的中心城市,故应加强对火电厂燃煤烟气中NOx的排放控制。     

广义系统具有完整性圆盘极点配置控制器设计

考虑线性连续广义系统具有完整性的圆盘极点配置设计问题·利用广义李亚普诺夫方程和不等式给出系统正则、无脉冲,以及极点在圆盘内的充分必要条件·同时针对执行器失效的线性连续广义系统,利用Riccati不等式给出具有执行器完整性圆盘极点配置控制器的设计方法,所设计的状态反馈控制器在系统正常时是正则、无脉冲、极点配置在圆盘内即系统是D允许的,在一定条件下,仍能使执行器故障具有D允许性     

一个人类锌指蛋白家族新基因ZFP28的克隆及表达

Krupple型锌指蛋白是一类具有重要功能的转录调节因子，初步克隆了一个新的人类krupple型锌指蛋白基因，经国际基因命我委员会批准命名为ZFP28。此基因长4076个碱基，含8个外显子，在基因组DNA上长18kb。它编码的蛋白质全长868个氨基酸，含1个信号肽，2个KRAB框和14个C2H2型的锌指。Northem Bolt分析表明该基因在人类早期胚胎的各个组织中普遍表达，在肺和肝中的表达水平最强，其结构和表达特征预示着它编码的是一个具DNA结合功能的转录调控因子。