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81.
阐述了多级自吸泵在节能与操作简便方面的实用意义,并且利用外混式自吸原理,将一台普通节段式多级离心泵改造成一台多级自吸离心泵,同时保持了原有的性能和安装尺寸。还分析了两端轴封漏气的原因,并提出了解决的方法。 相似文献
82.
Nuclear localization and signalling activity of phosphoinositidase C beta in Swiss 3T3 cells. 总被引:14,自引:0,他引:14
The hydrolysis of phosphatidylinositol 4,5-bisphosphate (PtdInsP2) is a widespread receptor-coupled signalling system at the plasma membrane of most eukaryotic cells. The existence of an entirely separate nuclear phosphoinositide signalling system is suggested from evidence that purified nuclei synthesize PtdInsP2 and phosphatidylinositol 4-phosphate (PtdInsP) in vitro and that a transient decrease in the mass of these lipids occurs when Swiss 3T3 cells are cultured in the presence of insulin-like growth factor-1 (IGF-1). These IGF-1-dependent changes in inositol lipids coincide with an increase in nuclear diacyglycerol and precede translocation to the nucleus and activation of protein kinase C (refs 5, 6). Circumstantial evidence that links these changes with mitosis comes from the isolation of a 3T3 clone that expresses the type-1 IGF receptor and binds IGF-1 peptide but does not respond mitogenically or show transient mass changes in nuclear inositol lipids. A key question is how IGF-1 initiates the rapid breakdown of PtdInsP and PtdInsP2 in the nucleus. Here we present evidence that nuclei of 3T3 cells contain the beta-isozyme of phosphoinositidase C, whereas the gamma-isozyme is confined to the cytoplasm and that IGF-1 treatment stimulates exclusively the activity of nuclear phosphoinositidase C. 相似文献
83.
Targeted disruption of the mouse transforming growth factor-beta 1 gene results in multifocal inflammatory disease. 总被引:149,自引:0,他引:149
M M Shull I Ormsby A B Kier S Pawlowski R J Diebold M Yin R Allen C Sidman G Proetzel D Calvin 《Nature》1992,359(6397):693-699
Transforming growth factor-beta 1 (TGF-beta 1) is a multifunctional growth factor that has profound regulatory effects on many developmental and physiological processes. Disruption of the TGF-beta 1 gene by homologous recombination in murine embryonic stem cells enables mice to be generated that carry the disrupted allele. Animals homozygous for the mutated TGF-beta 1 allele show no gross developmental abnormalities, but about 20 days after birth they succumb to a wasting syndrome accompanied by a multifocal, mixed inflammatory cell response and tissue necrosis, leading to organ failure and death. TGF-beta 1-deficient mice may be valuable models for human immune and inflammatory disorders, including autoimmune diseases, transplant rejection and graft versus host reactions. 相似文献
84.
樊永华 《山东大学学报(理学版)》1992,(1)
对工业生产过程控制和管理中的计算机图形显示,提出一种压缩存贮和滚动的新方法。新方法的实施,可使图形数据占用的存贮空间减少约80%,一般地不需使用外存,从而大大提高了显示速度,解决了普通微型机存贮空间和显示速度之间的矛盾,有效地提高了普通微机的功能/价格比。所提出的方法均经工程实践检验过,效果良好。 相似文献
85.
Short alanine peptides, containing 16 or 17 residues, appear to form alpha-helices in aqueous solution. But the main spectroscopic analyses used on helical peptides (circular dichroism and nuclear magnetic resonance) cannot distinguish between an alpha-helix (in which the ith residue is hydrogen-bonded to residue i + 4; ref. 9) and the next most common peptide helix, the 3(10)-helix10 (i-->i + 3 hydrogen-bonding). To address this problem we have designed single and doubly spin-labelled analogues of alanine-based peptides in which the nitroxide spin label forms an unbranched side chain extending from the sulphur atom of a cysteine residue. Here we report the circular dichroism, Fourier-transform infrared and electron-spin resonance spectra of these peptides under helix-forming conditions. The infrared absorbance gives an amide I' band with a frequency that is substantially different from that observed for alpha-helices. The electron-spin resonance spectra of doubly labelled helices show that the ranking of distances between side chains, around a single turn (residues 4-8), is inconsistent with an alpha-helical structure. Our experiments suggest that the more likely peptide geometry is a 3(10)-helix. 相似文献
86.
用“多化一”最小二乘法建立吸附动力学方程 总被引:1,自引:0,他引:1
樊恒鑫 《西北大学学报(自然科学版)》1995,25(4):289-292
用若干个一元最小二乘法,建立多元经验公式;以吸附动力学方程为例,取得了预期的结果。 相似文献
87.
高填方路基沉降处理及裂缝分析 总被引:1,自引:0,他引:1
分析了裂缝产生的原因,介绍了高填方路基沉降的处理方法。 相似文献
88.
A calcium sensor in the sodium channel modulates cardiac excitability. 总被引:11,自引:0,他引:11
Hanno L Tan Sabina Kupershmidt Rong Zhang Svetlana Stepanovic Dan M Roden Arthur A M Wilde Mark E Anderson Jeffrey R Balser 《Nature》2002,415(6870):442-447
Sodium channels are principal molecular determinants responsible for myocardial conduction and maintenance of the cardiac rhythm. Calcium ions (Ca2+) have a fundamental role in the coupling of cardiac myocyte excitation and contraction, yet mechanisms whereby intracellular Ca2+ may directly modulate Na channel function have yet to be identified. Here we show that calmodulin (CaM), a ubiquitous Ca2+-sensing protein, binds to the carboxy-terminal 'IQ' domain of the human cardiac Na channel (hH1) in a Ca2+-dependent manner. This binding interaction significantly enhances slow inactivation-a channel-gating process linked to life-threatening idiopathic ventricular arrhythmias. Mutations targeted to the IQ domain disrupted CaM binding and eliminated Ca2+/CaM-dependent slow inactivation, whereas the gating effects of Ca2+/CaM were restored by intracellular application of a peptide modelled after the IQ domain. A naturally occurring mutation (A1924T) in the IQ domain altered hH1 function in a manner characteristic of the Brugada arrhythmia syndrome, but at the same time inhibited slow inactivation induced by Ca2+/CaM, yielding a clinically benign (arrhythmia free) phenotype. 相似文献
89.
应用样条配点法以三次 B样条函数为时域函数的试函数 ,求解了反对称角铺设层合板的动力响应问题。在若干算例中 ,对于各种边界 ,选择样条函数作为振型函数 ,用样条配点法求出了结构的动力响应量 ,并与 Newmark法进行了比较。 相似文献
90.
采用随机域外奇点法对轴拉杆件进行了分析.考虑杨氏模量的不确定性,得到了不同相关类型、不同相关长度下的解析解,并分析了相关类型、相关长度以及随机场中点离散法对位移方差的影响. 相似文献