A rapid and accurate 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide colorimetric assay for quantification of bacteriocins with nisin as an example

The objective of this study is to propose a more accurate and faster MTT [3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide] colorimetric assay (MCA) for quantitative measurement of polypeptide bacteriocins in solutions with nisin as an example. After an initial incubation of nisin and indicator bacterium Micrococcus luteus NCIB 8166 in tubes, MTT was added for another incubation period. After that, nisin was quantified by estimating the number of viable bacteria based on measuring the amount of purple formazan produced by cleavage of yellow tetrazolium salt MTT. Then MCA was compared to a standard agar diffusion assay (ADA). The results suggested a high correlation coefficient (r ²=0.975±0.004) between optical density (OD) and the inhibitory effect of nisin on a bacterial strain Micrococcus luteus NCIB 8166 at a range of 0.125~32 IU/ml. The MCA described in this study was very quick. Quantification of nisin took only 7~8 h and the detection limit was at the level of 0.125 IU/ml when compared to 12 IU/ml and 24~28 h for ADA. The MCA provides an accurate and rapid method for quantification of nisin in solutions and is expected to be used for quantification of other antimicrobial substances.     

浅谈本科毕业设计(论文)质量的提高

分析了本科毕业设计(论文)工作的现状及普遍存在的问题,以南通大学为例,探讨了以本科教学水平评估为契机,切实提高毕业设计(论文)质量的方法。     

Amniotic membrane transplantation for porous sphere orbital implant exposure

Objective: This study is aimed at describing the clinical outcome of amniotic membrane transplantation for exposure of porous sphere implants. Methods: A retrospective review of consecutive cases of porous sphere orbital implant exposure was carried out. Eight cases were presented between May 2004 and Oct. 2006 (5 males, 3 females; mean age 44.5 years). Six had enucleation and two had evisceration. Exposure occurred in two primary and six secondary. Orbital implant diameter was 22 mm in seven cases and 20 mm in one case. Six patients are with hydroxyapatite and two with high-density porous polyethylene (Medpor) orbital implants. The mean time from implantation to exposure was 1.1 months (range 0.8~2 months). All patients required surgical intervention. Results: The time of follow-up ranged from 3.0 to 28.0 months (mean 16.5 months). Amniotic membrane grafting successfully closed the defect without re-exposure in all of these patients. The grafts were left bare with a mean time to conjunctiva of about 1 month (range 0.8~1.5 months). Conclusion: Exposed porous sphere implants were treated successfully with amniotic membrane graft in all of patients. The graft is easy to harvest. This technique is useful, dose not lead to prolonged socket inflammation and infection, and it is valuable application extensively.     

浅谈利用任务驱动法进行单片机教学与职业核心能力的培养

本文就任务驱动法在单片机教学中的应用与职业核心能力培养进行了阐述，并且以实例的形式得到了很好的验证。     

基于关联规则分析的组合拍卖竞胜标决定算法

组合拍卖竞胜标确定问题(WDP)是一个NP-完全问题,其算法研究一直是运筹学、人工智能和计算机科学等领域持续关注的热点.本文提出基于关联规则数据挖掘技术的组合拍卖竞胜标算法,并通过与经典的WDP精确算法和近似算法的比较,证明了该算法的有效性,为组合拍卖WDP算法提供了新的研究思路和方法.     

Excel在学生成绩统计分析中的综合应用

Excel在高校成绩统计分析中的应用非常普遍,但如何用好用活Excel值得交流.文章从个人的经验介绍了Excel在成绩统计分析中的灵活使用.     

有限群极大子群的CI-截

应用有限群极大子群的CI-截性质,讨论有限群G之正规子群H的可解性,得到4个重要结论.这4个结论推广了文献[3]的相关结果.     

与年龄相关的非线性时变种群系统的L2(Q)-解

本文讨论与年龄相关的非线性时变种群系统（P）的l^2（Q）－解，为进一步研究种群系统的最优控制问题提供了必要的理论基础．     

浅谈高校院系门户系统及其应用

介绍了高校院系门户系统的概念,分析了院系门户系统的结构,探讨了院系门户系统的实现和意义。