Molecular cloning and expression of interleukin Ibeta (IL-1β) from red seabream (Pagrus major)

The interleukin 1β (IL-1β) cDNA was cloned from the red seabream (Pagrus major) by homology cloning strategy. A cDNA fragment was amplified by PCR using two degenerated primers, which were designed according to the conserved regions of other known IL-1β sequences, and elongated by 3' ends and 5' ends RACE PCR to get the full length coding sequence of red seabream IL-1β (RS IL-1β). The sequence contained 1252 nucleotides that included a 5' untranslated region (UTR) of 84bp, a 3' UTR of 410 bp and an open reading frame (ORF) of 759 nucleotides which could be translated into a putative peptide of 253 amino acids with molecular weight of 28.6 kD and putative isoelectric point pI of 5.29. The deduced peptide contained two potential N-glycosylation sites and an identifiable IL1 family signature, but lacked the signal peptide and the clear ICE cut site, which were common in other nonmammalian IL-1β genes. The RS IL-1β had the highest homology with piscine IL-1β according to phylogenetic tree analysis.     

Development of Full Automatic External Welding Machine for Annular Welds of Pipeline

To meet the demands of rapid development of pipeline constructions in China, a complete set of full automatic external welding machine for annular welds of pipeline and welding procedure have been developed thereof. The system has been applied in the “West-East Pipeline Project” and its performance is proven. With this welding machine the welding parameters can be either preset before or adjusted in real time during the welding process, which avoids the blemishes of same type domestic and international products. Thus it is best propitious to in-situ construction conditions and workers‘ technical level in China. In this article the development of mechanical and control systems of this product is introduced and typical welding procedure parameters are provided.     

制备介孔复合环境材料的酸洗刻蚀工艺

探索了一种制备介孔复合环境材料的方法--破坏式造孔与有机复合相结合,使最终产品可大量吸附污染水体中的各种污染物并具有一定的杀菌功能.该方法通过酸洗刻蚀,达到了去除原材料中的杂质和刻蚀孔径的目的,所制备的中间产品24h吸湿率为原材料的2倍,染料吸附量为原材料的1.6倍.     

血压指标的聚类分析

利用353人的血压数据,运用2种不同的聚类分析方法对15个血压指标分类并挑选典型指标:一是利用SAS软件包中提供的变量聚类过程(VARCLUS过程)对指标进行裂类聚类分析;一是利用SAS中提供的样品聚类过程(CLUSTER过程)对指标进行系统聚类分析.2种方法得到了相同的结果:15个指标分成了4类,得到4个典型指标:BPR(收缩压与舒张压比值),BPP(收缩压与舒张压乘积),MAPPr(平均动脉压与脉率乘积),BPRPr(BPR与脉率乘积).     

极低硫钢的精炼脱硫动力学模型

通过对影响脱硫反应限制性环节的分析,将持续接触和瞬时接触两种反应模式综合考虑,建立了精炼脱硫动力学模型,并通过实验室"极低硫钢精炼脱硫"实验数据对模型进行验证.结果表明,本模型的预测结果与实验结果比较吻合.     

峰谷电价体制下东北输油管网日输油优化研究

东北原油长输管网是中国规模最大的地区性原油运输系统，就该管网基于峰谷电价体制下的运行现状，建立了线性规划数学模型，提出了日输油计划优化问题，并以铁秦线为例进行分析；初步揭示了峰谷电价体制下日输油计划方式的一些基本规律， 表明在该体制下东北管网具有一定的节能降耗潜力。但该方式在一定程度上受到管道本身运行特性的约束，与热油管道相比它更适合于等温管道。     

Spectroscopic study on the photophysical properties of chlorine substituted tetraphenylporphyrin-histidine and its zinc(Ⅱ)complexes

As the structural analogues of (bacterio)chlorophylls in photosynthetic organisms, porphyrins play an important role in building up artificial photosynthetic systems[13]. In natural photosynthetic pigment-protein complexes,certain amino acid residuals modulate the reactions ofcharge transfer and energy transfer via coordination andother types of interactions with chlorophyll molecules.For instance, in higher-plant photosynthetic reaction cen-ter PSⅡ, the oxidization-reduction system consistin…     

边界拟合坐标网格生成方法研究

指出了Thompson及Thomas曲线网格生成方法中控制网格分布的调节函数的问题所在，克服了Thomas曲线网格生成法中边界局部平直近似假定的缺陷，经过严格推导得出一组新的调节函数，并给出了曲线网格生成实例。实例检验表明，该调节函数能够对复杂边界的单连通域或多连通域生成理想的曲线网格，即边界处网格正交，内部网格分布能够适应物理量场变化。     

收尘极板上沉降粉尘粘结性能研究

振打清灰效果不好，严重影响电除尘器的除尘效率和使用范围，而影响振打清灰效果的最主要因素是沉降粉体的粘结力。通过建立数学模型从微观的角度推导出粉尘之间各种粘结力的理论计算公式。分析了比电阻、外加电场和粉尘含水率等因素对粉尘粘结力的影响。在实验中，模拟电除尘器中的静电场，测出在不同外加电场下粉尘粘结力的大小，定量的反应了外加电场对粉尘粘结性能的影响。     

抽水引起岩土体内应力变化分析

针对开采地下水这一特定问题,以在抽水实践中地下水水位面是一以井心为顶点的旋转抛物面为出发点,研究了地下水开采过程中岩土体应力变化的时空规律。通过分析影响内部应力变化的宏观物理量—开采影响半径、井心水位下降和空间位置,可以方便的得到随着时间变化岩土体内部应力变化的全程规律。同时,分析了影响边界、下沉位置等因素对计算结果的影响。