D-海因酶基因重组子的构建和酶的表达活性

以中华根瘤菌(Sinorhizobium morelense)SS—ori总DNA为模板，用PCR法扩增D-海因酶基因，分别克隆到5种不同的载体，并转入5种不同的Escherichia coli菌株，获得25株工程菌，进行培养与诱导表达．细胞经超声处理后，通过SDS—PAGE和酶活性两种指标比较表达产物．结果表明，除3株工程菌具有D-海因酶活性外，其它均为无酶活性的不溶性包涵体，包涵体经变性、复性后，可部分获得有活性的D-海因酶，其比活为0．90U／mg，复性率约为20％．此外，对包涵体产生的原因及可能解决办法也进行了讨论．     

高浓度氨氮废水处理技术

综述了几种高浓度氨氮废水处理技术,介绍了它们各自的优势和适用范围,报道了高氨氮废水处理的研究进展,指出了各种技术在今后研究中需要解决的问题.     

3104深冲铝板织构对初始R值的影响

以深冲铝板R值的在线检测为应用背景,塑性变形滑移系开动的过程为出发点,分析了初始滑移系选取的方式,并计算了不同取向3104铝合金板的初始R值.结果表明,织构是决定板材R值最主要的因素,其中初始R值是实测R值的重要组成部分.3104铝板中存在的织构组分决定其R0值最低、R90值最高.计算表明,{111}面织构有利于在明显降低制耳效应的前提下,大幅度提高铝板的深冲压性能.     

基于虚拟仪器的超声Doppler管道低流速测量

传统的超声Doppler流量计在管道流量测量中会遇到无法判断流速方向和低流速测量困难等问题。该文基于虚拟仪器平台,将Doppler信号解调到15kHz的中心频率,并运用Zoom-FFT算法对其进行高精度选带频谱分析,然后进行加权平均频率估计,得到被测流体的平均流速及方向,实现了一个新的超声Doppler流量测量系统。超声波探头外夹安装在充满流体介质的不锈钢管道上进行流量测量,实验结果表明该系统不仅可以正确分辨流速方向,还能测量低达0.02m/s的流速,在判断流速方向和低流速测量上比传统超声Doppler流量计更为有效。     

定宽机主曲柄初始位置对模块有效行程的影响

系统地模拟了SP定宽机同步机构和侧压机构的运动规律，采用Powell优化理论，以模块最大有效行程为目标函数，以主曲柄初始角度和偏心距为变量进行优化分析，得到一组最优解。该结论为现场控制定宽机主曲柄的初始位置提供了理论依据。     

大型餐饮软件系统的信息管理与实现

由于现有大型餐饮管理软件系统没有很好地考虑对象本身自有的规律，成功的案例很少．根据一大型餐饮管理软件系统的设计与实现，从信息控制角度阐述了一些独特的系统设计思路．实践证明，这些方法的应用不仅大幅度地提高了该企业的工作效率，而且提高了员工的工作积极性和主动性．很好地实现了系统开发目的．     

服装风格的量化方法初探

为使企业能够自主控制服装风格，减少由设计师更换带来的品牌风格波动，使企业决策者能够把握服装的设计风格，尝试对服装风格进行量化，以找到品牌服装风格的内在规律，使服装的风格控制成为一种技术手段。通过多次实验，找到较可行的量化方法并进行初步量化，得到初步量化结论。     

Characterization of polyphenol oxidase from plants

Polyphenol oxidase (PPO) which can mediate browning reaction is a bifunctional copper-containing enzyme encoded by plant nucleolus gene. It usually leads to excessive browning reaction which reduces the coercial profits of fruits and vegetables. In this paper, PPO genes and enzymes in plants are characterized systematically, and the latest progress is reviewed. Some clonings of PPOs genes are reported; the specific temporal and spatial expression pattern of PPOs genes is described; the model of the structure of the precursor form of catechol oxidase is introduced; the possible functions of PPOs in defending against pathogen, wounding, surrounding stress and other inducing factors are demonstrated; the induction and activation of latent PPOs in some plants is elucidated; the scheme of browning inhibition by L-cysteine is clarified; the mechanism of suicide inhibition of latent PPO and kinetic synergism are established. Furthermore, the area for future study is also discussed.     

Relationship between the exposure dose of high intensity focused ultrasound and the heated necrosis element

Heated necrosis element of tissues is the basis for treating tumor by high intensity focused ultrasound (HIFU), so study on the relationship between the HIFU exposure dose and the heated necrosis element of tissues is of great significance for both HIFU clinical applications and basic research. Using Pennes and Rayleigh formulae the relationship between the exposure dose of an annular focused transducer and the heated necrosis element (I.e. Biological focused region) in a bovine liver in vitro at a depth of 2 cm is simulated in an exposure dose range with radiation sound intensity of 7000～25400 W/cm2 and exposure time of 0～20 s. The theoretical results appear to be in quite good agreement with the experimental ones. It is shown that on the basis of existing theories the heated necrosis element produced by HIFU exposure can be predicted. The deviation of the theory from the experiments under higher exposure dose has also been discussed.     

目标数量折扣下的供应链协调分析

目前国内一些行业的公司在销售渠道中常用目标数量折扣的契约模式。本文通过一个两阶段一对一的供应链模型分析了在这种契约下供应链的协调性，以及它对供应链利润分配的影响。最后针对某品牌型号的手机进行了实证分析。