全文获取类型
收费全文 | 345篇 |
免费 | 0篇 |
国内免费 | 1篇 |
专业分类
系统科学 | 5篇 |
理论与方法论 | 2篇 |
现状及发展 | 49篇 |
研究方法 | 37篇 |
综合类 | 241篇 |
自然研究 | 12篇 |
出版年
2019年 | 2篇 |
2017年 | 1篇 |
2016年 | 2篇 |
2015年 | 2篇 |
2013年 | 1篇 |
2012年 | 16篇 |
2011年 | 24篇 |
2010年 | 4篇 |
2009年 | 2篇 |
2008年 | 11篇 |
2007年 | 20篇 |
2006年 | 13篇 |
2005年 | 14篇 |
2004年 | 10篇 |
2003年 | 4篇 |
2002年 | 8篇 |
2001年 | 7篇 |
2000年 | 13篇 |
1999年 | 2篇 |
1997年 | 1篇 |
1996年 | 1篇 |
1994年 | 1篇 |
1992年 | 11篇 |
1991年 | 5篇 |
1990年 | 12篇 |
1989年 | 8篇 |
1988年 | 4篇 |
1987年 | 8篇 |
1986年 | 12篇 |
1985年 | 9篇 |
1984年 | 3篇 |
1983年 | 5篇 |
1982年 | 6篇 |
1981年 | 4篇 |
1980年 | 1篇 |
1979年 | 7篇 |
1978年 | 3篇 |
1977年 | 9篇 |
1976年 | 3篇 |
1975年 | 6篇 |
1974年 | 10篇 |
1973年 | 5篇 |
1972年 | 7篇 |
1971年 | 11篇 |
1970年 | 7篇 |
1969年 | 3篇 |
1968年 | 9篇 |
1967年 | 8篇 |
1966年 | 4篇 |
1965年 | 6篇 |
排序方式: 共有346条查询结果,搜索用时 31 毫秒
111.
112.
113.
A physical map of the mouse genome 总被引:1,自引:0,他引:1
Gregory SG Sekhon M Schein J Zhao S Osoegawa K Scott CE Evans RS Burridge PW Cox TV Fox CA Hutton RD Mullenger IR Phillips KJ Smith J Stalker J Threadgold GJ Birney E Wylie K Chinwalla A Wallis J Hillier L Carter J Gaige T Jaeger S Kremitzki C Layman D Maas J McGrane R Mead K Walker R Jones S Smith M Asano J Bosdet I Chan S Chittaranjan S Chiu R Fjell C Fuhrmann D Girn N Gray C Guin R Hsiao L Krzywinski M Kutsche R Lee SS Mathewson C McLeavy C Messervier S Ness S Pandoh P Prabhu AL Saeedi P 《Nature》2002,418(6899):743-750
A physical map of a genome is an essential guide for navigation, allowing the location of any gene or other landmark in the chromosomal DNA. We have constructed a physical map of the mouse genome that contains 296 contigs of overlapping bacterial clones and 16,992 unique markers. The mouse contigs were aligned to the human genome sequence on the basis of 51,486 homology matches, thus enabling use of the conserved synteny (correspondence between chromosome blocks) of the two genomes to accelerate construction of the mouse map. The map provides a framework for assembly of whole-genome shotgun sequence data, and a tile path of clones for generation of the reference sequence. Definition of the human-mouse alignment at this level of resolution enables identification of a mouse clone that corresponds to almost any position in the human genome. The human sequence may be used to facilitate construction of other mammalian genome maps using the same strategy. 相似文献
114.
Changing potency by spontaneous fusion 总被引:204,自引:0,他引:204
Recent reports have suggested that mammalian stem cells residing in one tissue may have the capacity to produce differentiated cell types for other tissues and organs 1-9. Here we define a mechanism by which progenitor cells of the central nervous system can give rise to non-neural derivatives. Cells taken from mouse brain were co-cultured with pluripotent embryonic stem cells. Following selection for a transgenic marker carried only by the brain cells, undifferentiated stem cells are recovered in which the brain cell genome has undergone epigenetic reprogramming. However, these cells also carry a transgenic marker and chromosomes derived from the embryonic stem cells. Therefore the altered phenotype does not arise by direct conversion of brain to embryonic stem cell but rather through spontaneous generation of hybrid cells. The tetraploid hybrids exhibit full pluripotent character, including multilineage contribution to chimaeras. We propose that transdetermination consequent to cell fusion 10 could underlie many observations otherwise attributed to an intrinsic plasticity of tissue stem cells 9. 相似文献
115.
Kodama R Norreys PA Mima K Dangor AE Evans RG Fujita H Kitagawa Y Krushelnick K Miyakoshi T Miyanaga N Norimatsu T Rose SJ Shozaki T Shigemori K Sunahara A Tampo M Tanaka KA Toyama Y Yamanaka T Zepf M 《Nature》2001,412(6849):798-802
Modern high-power lasers can generate extreme states of matter that are relevant to astrophysics, equation-of-state studies and fusion energy research. Laser-driven implosions of spherical polymer shells have, for example, achieved an increase in density of 1,000 times relative to the solid state. These densities are large enough to enable controlled fusion, but to achieve energy gain a small volume of compressed fuel (known as the 'spark') must be heated to temperatures of about 108 K (corresponding to thermal energies in excess of 10 keV). In the conventional approach to controlled fusion, the spark is both produced and heated by accurately timed shock waves, but this process requires both precise implosion symmetry and a very large drive energy. In principle, these requirements can be significantly relaxed by performing the compression and fast heating separately; however, this 'fast ignitor' approach also suffers drawbacks, such as propagation losses and deflection of the ultra-intense laser pulse by the plasma surrounding the compressed fuel. Here we employ a new compression geometry that eliminates these problems; we combine production of compressed matter in a laser-driven implosion with picosecond-fast heating by a laser pulse timed to coincide with the peak compression. Our approach therefore permits efficient compression and heating to be carried out simultaneously, providing a route to efficient fusion energy production. 相似文献
116.
117.
Burrows DN Kennea JA Ghisellini G Mangano V Zhang B Page KL Eracleous M Romano P Sakamoto T Falcone AD Osborne JP Campana S Beardmore AP Breeveld AA Chester MM Corbet R Covino S Cummings JR D'Avanzo P D'Elia V Esposito P Evans PA Fugazza D Gelbord JM Hiroi K Holland ST Huang KY Im M Israel G Jeon Y Jeon YB Jun HD Kawai N Kim JH Krimm HA Marshall FE P Mészáros Negoro H Omodei N Park WK Perkins JS Sugizaki M Sung HI Tagliaferri G Troja E Ueda Y Urata Y Usui R Antonelli LA Barthelmy SD Cusumano G 《Nature》2011,476(7361):421-424
Supermassive black holes have powerful gravitational fields with strong gradients that can destroy stars that get too close, producing a bright flare in ultraviolet and X-ray spectral regions from stellar debris that forms an accretion disk around the black hole. The aftermath of this process may have been seen several times over the past two decades in the form of sparsely sampled, slowly fading emission from distant galaxies, but the onset of the stellar disruption event has not hitherto been observed. Here we report observations of a bright X-ray flare from the extragalactic transient Swift J164449.3+573451. This source increased in brightness in the X-ray band by a factor of at least 10,000 since 1990 and by a factor of at least 100 since early 2010. We conclude that we have captured the onset of relativistic jet activity from a supermassive black hole. A companion paper comes to similar conclusions on the basis of radio observations. This event is probably due to the tidal disruption of a star falling into a supermassive black hole, but the detailed behaviour differs from current theoretical models of such events. 相似文献
118.
119.
120.