Conservation, status, and life history of the endangered White River spinedace, Lepidomeda albivallis (Cyprinidae)

Lepidomeda albivallis (White River spinedace), a fish species endemic to the White River, Nevada, appeared headed toward extinction. In 1991 only 1 population remained, and it comprised fewer than 50 individuals in a 70-m stream reach. We monitored population recruitment and distribution and studied life history and habitat use from 1993 through 1998. We determined that L. albivallis was not reproducing and was continuing to decline, and as an emergency measure we relocated the population (14 in spring 1995 and 6 in spring 1996) downstream 200 m to a secure habitat that we judged more favorable for reproduction. The relocated population reproduced, and by September 1998 it had increased to 396 individuals that inhabited more than 1 km of stream including both pond and stream habitats. In streams they oriented near the bottom but frequently moved up in the water column to strike at drift items. Gut analysis of museum specimens indicated L. albivallis is omnivorous but feeds primarily upon aquatic invertebrates. Conservation of L. albivallis will require reestablishing additional populations within its former range.     

Schools as Communities: Purveyors of Democratic Values and the Cornerstones of a Public Philosophy

Establishing interdependency and community among people may be the solution to reestablishing or creating a tighter fabric of support for childrens' development, for building cultural democracy and a democratic state. This paper attempts to create a bridge between education and the construction of sustainable democratic communities in education, as a basis for building a public philosophy. Ultimately, sustainable democratic educational communities may address the concerns of today and tomorrow and build sustainable democratic communities in the larger global society.     

Model of breeding habitat of the Mountain Plover ( Charadrius montanus ) in western Wyoming

To aid the conservation and management of Mountain Plovers ( Charadrius montanus ), we developed a spatially explicit model of breeding habitat for the shrub-steppe of western Wyoming. Points of Mountain Plover presence and absence were determined by field surveys conducted May through June 1999. At each point we measured topographic slope and a cover type suitability index related to vegetation height and density. Logistic regression revealed a strong negative relationship between presence and slope, and a weaker and positive relationship between presence and cover index. A multiple logistic regression model using slope and cover index to predict presence successfully classified 87% of the points in an independent data set covering much of western Wyoming. The spatial expression of this model will help managers target future surveys and identify currently unsuitable habitat that could be improved via vegetation management. Patches of suitable breeding habitat of the Mountain Plover in western Wyoming are probably functions of poor soil, low precipitation, and wind scour, and thus are likely rather persistent in space and time. Combined with the relatively large proportion of publicly owned land here, this may make conservation and recovery of the Mountain Plover easier in western Wyoming than in the more dynamic and privately controlled Great Plains.     

Interactive Management: An Emancipatory Methodology

An erosion of the power of people and their communities to control any more than a fragment of their future continues. Educational and governmental policy decisions are, for the most part, mandated from above and/or outside the community. This paper introduces a model which reflects Freire's humanizing pedagogy. This method is a vehicle, an avenue of empowerment for people and their communities to control their own destiny and the future of their community. The model is Interactive Management (IM) and its derivative, The CogniScope System. IM is a specialized system of management using collaborative teamwork to define and resolve highly complex issues. The process includes the integration of the diversity of perceptions of the participants with regard to the given ssues, builds consensus and joint ownership of the process and/or product, and creates a collaborative action plan to accomplish the participants' goals. The principal intangible outcome of the process, however, is the reflective participation, which leads to action, and the learning that occurs among the community of stakeholders present. The process of Interactive Management (IM), enhanced through the use of the CogniScope System, is described and examples of its application by First Nation peoples are shared.     

Tunable gold catalysts for selective hydrocarbon oxidation under mild conditions

Oxidation is an important method for the synthesis of chemical intermediates in the manufacture of high-tonnage commodities, high-value fine chemicals, agrochemicals and pharmaceuticals: but oxidations are often inefficient. The introduction of catalytic systems using oxygen from air is preferred for 'green' processing. Gold catalysis is now showing potential in selective redox processes, particularly for alcohol oxidation and the direct synthesis of hydrogen peroxide. However, a major challenge that persists is the synthesis of an epoxide by the direct electrophilic addition of oxygen to an alkene. Although ethene is epoxidized efficiently using molecular oxygen with silver catalysts in a large-scale industrial process, this is unique because higher alkenes can only be effectively epoxidized using hydrogen peroxide, hydroperoxides or stoichiometric oxygen donors. Here we show that nanocrystalline gold catalysts can provide tunable active catalysts for the oxidation of alkenes using air, with exceptionally high selectivity to partial oxidation products ( approximately 98%) and significant conversions. Our finding significantly extends the discovery by Haruta that nanocrystalline gold can epoxidize alkenes when hydrogen is used to activate the molecular oxygen; in our case, no sacrificial reductant is needed. We anticipate that our finding will initiate attempts to understand more fully the mechanism of oxygen activation at gold surfaces, which might lead to commercial exploitation of the high redox activity of gold nanocrystals.     

Casein kinase 1 gamma couples Wnt receptor activation to cytoplasmic signal transduction

Signalling by Wnt proteins (Wingless in Drosophila) has diverse roles during embryonic development and in adults, and is implicated in human diseases, including cancer. LDL-receptor-related proteins 5 and 6 (LRP5 and LRP6; Arrow in Drosophila) are key receptors required for transmission of Wnt/beta-catenin signalling in metazoa. Although the role of these receptors in Wnt signalling is well established, their coupling with the cytoplasmic signalling apparatus remains poorly defined. Using a protein modification screen for regulators of LRP6, we describe the identification of Xenopus Casein kinase 1 gamma (CK1gamma), a membrane-bound member of the CK1 family. Gain-of-function and loss-of-function experiments show that CK1gamma is both necessary and sufficient to transduce LRP6 signalling in vertebrates and Drosophila cells. In Xenopus embryos, CK1gamma is required during anterio-posterior patterning to promote posteriorizing Wnt/beta-catenin signalling. CK1gamma is associated with LRP6, which has multiple, modular CK1 phosphorylation sites. Wnt treatment induces the rapid CK1gamma-mediated phosphorylation of these sites within LRP6, which, in turn, promotes the recruitment of the scaffold protein Axin. Our results reveal an evolutionarily conserved mechanism that couples Wnt receptor activation to the cytoplasmic signal transduction apparatus.     

The reversibility of mitotic exit in vertebrate cells

A guiding hypothesis for cell-cycle regulation asserts that regulated proteolysis constrains the directionality of certain cell-cycle transitions. Here we test this hypothesis for mitotic exit, which is regulated by degradation of the cyclin-dependent kinase 1 (Cdk1) activator, cyclin B. Application of chemical Cdk1 inhibitors to cells in mitosis induces cytokinesis and other normal aspects of mitotic exit, including cyclin B degradation. However, chromatid segregation fails, resulting in entrapment of chromatin in the midbody. If cyclin B degradation is blocked with a proteasome inhibitor or by expression of non-degradable cyclin B, Cdk inhibitors will nonetheless induce mitotic exit and cytokinesis. However, if after mitotic exit, the Cdk1 inhibitor is washed free from cells in which cyclin B degradation is blocked, the cells can revert back to M phase. This reversal is characterized by chromosome recondensation, nuclear envelope breakdown, assembly of microtubules into a mitotic spindle, and in most cases, dissolution of the midbody, reopening of the cleavage furrow, and realignment of chromosomes at the metaphase plate. These findings demonstrate that proteasome-dependent degradation of cyclin B provides directionality for the M phase to G1 transition.     

Analysis of the DNA sequence and duplication history of human chromosome 15

Here we present a finished sequence of human chromosome 15, together with a high-quality gene catalogue. As chromosome 15 is one of seven human chromosomes with a high rate of segmental duplication, we have carried out a detailed analysis of the duplication structure of the chromosome. Segmental duplications in chromosome 15 are largely clustered in two regions, on proximal and distal 15q; the proximal region is notable because recombination among the segmental duplications can result in deletions causing Prader-Willi and Angelman syndromes. Sequence analysis shows that the proximal and distal regions of 15q share extensive ancient similarity. Using a simple approach, we have been able to reconstruct many of the events by which the current duplication structure arose. We find that most of the intrachromosomal duplications seem to share a common ancestry. Finally, we demonstrate that some remaining gaps in the genome sequence are probably due to structural polymorphisms between haplotypes; this may explain a significant fraction of the gaps remaining in the human genome.