Stability of the Sarcin/Ricin domain of 28S RNA in rat ribosome under hydrostatic pressure

Under 2 100 bar of hydrostatic pressure, KNA N-glycosidase can still cleave the N-C bond of adenosine at position 4324 in R/S domain of 28S RNA in rat ribosome, demonstrating that high pressure has no effect on the tertiary structure of S/R domain.     

Kinetic model for the smectite to illite transformation in active geothermal systems

The smectite to illite transformation in active geothermal systems of New Zealand can be simulated by a first-order reaction kinetic model, which provides direct estimates about the minumum ages of active geothermal systems themselves. The derived kinetic values show that the smectite to illite transformation is sensitive to both temperature and time.     

用^1H—NMR测定纤维素的摩尔取代度

用＾１Ｈ－ＮＭＲ测定羟丙基纤维素的摩尔取代度,根据纤维素在ＤＣｌ中水解后的＾１Ｈ－ＮＭＲ谱峰面积来计算ＭＳ值。同时还应用铬酸氧化蒸馏法测定了羟丙基纤维素的ＭＳ值,以及利用ＭＳ与羟丙基含量的关系得到ＭＳ值,可以发现ＮＭＲ的方法是可行的。     

应变速率对8090Al-Li合金拉伸性能的影响

用动态拉伸的方法研究了应变速率对 80 90 Al— L i合金拉伸性能的影响 ,应变速率的范围为 10 - 3～ 10 3s- 1。结果表明 ,合金的强度和塑性随应变率的增加而增加。试样的 SEM和 TEM分析发现 :准静态下的断裂为沿晶断裂 ;随着应变率的增加 ,断裂从穿晶准解理断裂过渡到由显微空穴聚集而成的韧窝断裂。高应变率下80 90 Al- L i合金强度和塑性的提高与其变形机制和晶界无沉淀区 (PFZ)的变化有关     

链条锅炉模糊控制研究

针对链条锅炉的运行特点,提出一套基于模糊控制理论的链条锅炉控制方案。链条锅炉滞后时间长、惯性大,采用传统控制方法控制效果不理想,但采用模糊控制方法可以取得良好的控制效果。本控制系统由负荷控制和燃烧控制子系统构成。控制系统通过调节供入的燃料量和合理组织炉内燃烧,提高了锅炉的运行效率。针对不同的调节对象,分别设计了不同的模糊控制器,改善了控制系统的整体控制效果。该系统已经成功地应用于北京市德宝锅炉房。     

美东地区罗德河流域地下水中铝形态分布的化学平衡模式计算

美东地区的罗德河流域遭受着严重的酸沉降侵蚀．在酸性条件下,铝从土壤中大量溶解并迁移到水环境中．地表水中升高的铝浓度对鱼类产生毒害,监测地表水和土壤水中的铝形态及浓度变化已成为问题的关键．本文报导了用化学平衡模式计算法分析美东地区罗德河流域地下水中的铝形态分布,获得了有价值的结论     

A massive phytoplankton bloom induced by an ecosystem-scale iron fertilization experiment in the equatorial Pacific Ocean

The seeding of an expanse of surface waters in the equatorial Pacific Ocean with low concentrations of dissolved iron triggered a massive phytoplankton bloom which consumed large quantities of carbon dioxide and nitrate that these microscopic plants cannot fully utilize under natural conditions. These and other observations provide unequivocal support for the hypothesis that phytoplankton growth in this oceanic region is limited by iron bioavailability.     

Insect motion detectors matched to visual ecology

To detect motion, primates, birds and insects all use local detectors to correlate signals sampled at one location in the image with those sampled after a delay at adjacent locations. These detectors can adapt to high image velocities by shortening the delay. To investigate whether they use long delays for detecting low velocities, we compared motion-sensitive neurons in ten species of fast-flying insects, some of which encounter low velocities while hovering. Neurons of bee-flies and hawkmoths, which hover, are tuned to lower temporal frequencies than those of butterflies and bumblebees, which do not. Tuning to low frequencies indicates longer delays and extends sensitivity to lower velocities. Hoverflies retain fast temporal tuning but use their high spatial acuity for sensing low-velocity motion. Thus an unexpectedly wide range of spatio-temporal tuning matches motion detection to visual ecology.     

The use of cyclophosphamide as an enhancer of the vaccine against foot-and-mouth disease

The immunization of biungulate animals with killed foot-and-mouth disease virus (FMDV) requires periodic vaccinations due to a low vaccine immunogenicity. Therefore, FMDV antigens need to be combined with adjuvants such as aluminum hydroxide, saponin or oil emulsions. Animal handling for periodic inoculations, and the repeated doses of vaccines that have to be administered increase the commercialization costs. Moreover, the use of adjuvants may induce adverse effects.In the present work we show that it is possible to increase the life span of neutralizing antibodies in serum when a single dose of cyclophosphamide (Cy) is administered four days before vaccination with aluminum hydroxidesaponin FMDV vaccine.     

Some citrus chitinases also possess chitosanase activities

Several acidic chitinase and chitosanase isoforms were found in 4-week-old nonembryogenic sweet orange (Valencia [Citrus sinensis (L.) Osbeck]) callus tissue. Two isoforms (designated A1-CF1 and A1-CF2) were purified to homogeneity using HPLC size exclusion, anion exchange, and chromatofocusing techniques. Both hydrolase isoforms exhibited activity with either colloidal chitin or solubilized shrimp shell chitosan. Specific activities for the purified isoforms could not be calculated because of the lack of protein and contamination of ampholytes. However, the specific activities for chitinase and chitosanase after anion exchange were respectively 404 nmol GlcNAc per min per mg protein and 2,475 nmol GlcN per min per mg protein. The M_r for both enzymes was 30,500. The homogeneous proteins cross-reacted in western blots with antiserum against a basic class I potato leaf chitinase.