Antioxidant survey to assess antagonism to redox stress using a prokaryotic and an eukaryotic system

Using a prokaryote (Escherichia coli) and a metazoa-resembling eukaryote (Ochromonas danica), we surveyed antioxidants which might overcome redox stress imposed by menadione sodium bisulphite (MD) and buthionine sulphoximine (BSO). BSO oxidant stress was evident only inO. danica; MD oxidant stress was evident in both organisms. Glutathione, its precursors, e.g. cysteine, homocysteine, and 2-oxo-4-thiazolidine carboxylic acid, and red blood cells, emerged as prime antioxidants for relieving BSO and MD oxidant stress. BSO and MD oxidant activity and antioxidant-annulling effect inO. danica were judged comparable to those found in animal cells whereas the resultsE. coli were not entirely equivalent. TheO. danica system emerged as a practical, rapid, and useful system for pinpointing oxidant stressors and antioxidants, and shows promise for studies with mammalian systems.     

(−)-Epigallocatechin gallate,a polyphenolic tea antioxidant,inhibits peroxynitritemediated formation of 8-oxodeoxyguanosine and 3-nitrotyrosine

Reaction with peroxynitrite at pH 7.4 and 37°C was found to increase the 8-oxodeoxyguanosine levels in calf thymus DNA 35-38-fold. This oxidation of deoxyguanosine, as well as the peroxynitrite-mediated nitration of tyrosine to 3-nitrotyrosine, was significantly inhibited by ascorbic acid, glutathione and (–)-epigallocatechin gallate, a polyphenolic antioxidant present in tea. For 50% inhibition of the oxidation of deoxyguanosine to 8-oxodeoxyguanosine, 1.1, 7.6 of 0.25 mM ascorbate, glutathione or (–)-epigallocatechin gallate, respectively, was required. For 50% inhibition of tyrosine nitration, the respective concentrations were 1.4, 4.6 or 0.11 mM. Thus, (–)-epigallocatechin gallate is a significantly better inhibitor of both reactions than either ascorbate or glutathione. Reaction of (–)-epigallocatechin gallate with peroxynitrite alone resulted in the formation of a number of products. Ultraviolet spectra of two of these suggest that the tea polyphenol and/or its oxidation products are nitrated by peroxynitrite.     

工业化初期墨西哥地方市政府决策之经济人类学研究

向城市贫困人口提供廉价的生活物资和基本服务是发展中国家面临的一个主要问题,尤其是在工业化的早期。从经济角度来讲,向这一群体提供服务是至关重要的,因为大部分的劳动力来自于这一群体,而劳动力是国家工业化的一个要素。从历史角度来看,向这一群体提供服务属于地方市政府的职责。因此,通过对地方政府决定的研究,可以找到与提供生活必需品政策相关的问题的最佳答案。工业化发展初期的墨西哥,是研究此类动态变化的理想之地。     

家庭装潢中水管的选择

在室内装潢方面，人们较注重室内布局和装饰，而对给水管道则极少关注．应引起足够的重视。在建筑给水系统中由新型塑料管材替代金属管材已成为趋势。所以，室内给水管应选用新型塑料管材，并且应根据新型塑料管材的特点进行施工。确保给水系统的安装质量。     

A novel audio watermarking scheme using multiscale wavelet modulation

A novel audio watermarking scheme to embed robust and inaudible watermarks for the purpose of copyright protection is proposed. The key innovation is to add time-frequency redundancy into watermark signals by multiscale wavelet modulation. In order to maximize the watermarking strength within perceptual constraints, the signals synthesized from different scales are masked using a frequency auditory model, respectively, and then intergrated to form the final watermark signal. The detection structure is built using the redundancy in watermark signals, and the performance is further enhanced by modeling the statistical behaviors of wavelet coefficients as generalized Gaussian distribution. The use of original audio signal is not required in watermark detection. The experimental results show that our approach can achieve not only good transparency but also satisfying robustness to common audio manipulations.     

无功就地补偿技术在煤矿井下的应用前景

对无功就地补偿技术在煤矿供电系统的应用中进行了分析,指出该技术能够起到节约电能、提高供电质量、节约材料费等作用,阐述了无功就地补偿技术在煤矿供电系统中的应用有较好的发展前景。     

Complex reorganization and predominant non-homologous repair following chromosomal breakage in karyotypically balanced germline rearrangements and transgenic integration

We defined the genetic landscape of balanced chromosomal rearrangements at nucleotide resolution by sequencing 141 breakpoints from cytogenetically interpreted translocations and inversions. We confirm that the recently described phenomenon of 'chromothripsis' (massive chromosomal shattering and reorganization) is not unique to cancer cells but also occurs in the germline, where it can resolve to a relatively balanced state with frequent inversions. We detected a high incidence of complex rearrangements (19.2%) and substantially less reliance on microhomology (31%) than previously observed in benign copy-number variants (CNVs). We compared these results to experimentally generated DNA breakage-repair by sequencing seven transgenic animals, revealing extensive rearrangement of the transgene and host genome with similar complexity to human germline alterations. Inversion was the most common rearrangement, suggesting that a combined mechanism involving template switching and non-homologous repair mediates the formation of balanced complex rearrangements that are viable, stably replicated and transmitted unaltered to subsequent generations.     

基于Web的卫星云图信息系统研究

1　项目背景　　最近 ,我们为民航空管局开发了综合业务网络系统 .系统的基本构架是浏览器 /服务器 (Ｂ/Ｓ)模式 ,采用Ｓｕｎ公司的超级小型机作为Ｗｅｂ服务器和数据库服务器 ,操作系统为ＳｕｎＳｏｌａｒｉｓ 8;Ｗｅｂ服务器程序使用Ａ ｐａｃｈｅ ,开发语言使用ＰＨＰ和Ｊａｖａ ;数据库系统采用Ｏｒａｃｌｅ 8.1.6ＥｎｔｅｒｐｒｉｓｅＥｄｉｔｉｏｎ .综合业务网络系统的主要功能 ,是将空管局目前几个单独运行的业务系统信息综合起来 ,为空管局的日常工作以及航空公司和驻场单位等业务数据用户提供及时、准确、综合的信息 .其中一项重…