Metachromasia in cultured fibroblasts of subjects with glycogenosis type II

Riassunto Frammenti di cute di due soggetti affetti da glicogenosi tipo II e dei loro genitori e fratelli sono stati coltivati in vitro. Sia nelle culture dei pazienti che dei loro familiari si è osservata la presenza di materiale metacromatico dopo colorazione con blu di toluidina 0. Nei leucociti di tutti i familiari esaminati l'attività della -1,4-glucosidasi è risultata ridotta.     

Confirmation of sex pheromone biosynthesis from (16-D3)palmitic acid in the turnip moth using capillary gas chromatography

Summary (16-D₃)palmitic acid was applied topically to pheromone glands of the turnip moth. After incubation omega labeled analogues of fatty acid methyl esters and acetates were identified by gas chromatography with flame ionization detection and selected ion monitoring. The pheromone components (Z)-5-decenyl, (Z)-7-dodecenyl- and (Z)-9-tetradecenyl acetate were all biosynthesized from palmitic acid along a common pathway.     

Influence of hydrocortisone on cytopathic effect of Newcastle disease virus and stability to freezing of vescicular stomatitis virus

Summary The presence of hydrocortisone in virus-infected cell cultures leads to enhancement of the syncytia forming ability of Newcastle disease virus and to production of vescicular stomatitis virus particles which loose their infectivity upon storage below 0°C.This work was supported by the Italian Consiglio Nazionale delle Ricerche (CNR), Progetto finalizzato Virus, Contract N. 77.00284.84.     

A simple system of multiple organ-baths

Summary A compact and easily assembled system of multiple organ-baths is described, in which use is made of readily available materials.Acknowledgment. G.E.R. was in receipt of the ruggles Gates Research Award.     

Developmental characteristics of histamine methyltransferase and phenylethanolamine-N-methyltransferase of rat brain

Summary The specific activity of histamine methyltransferase of rat brain increases rapidly from the 16th until the 25th day of gestation (7 days after birth). The specific activity of phenylethanolamine-N-methyl-transferase shows a rapid incrase during the 1st and the 2nd week after birth, the adult values being obtained by the end of the 2nd week.