全文获取类型
收费全文 | 5318篇 |
免费 | 15篇 |
国内免费 | 32篇 |
专业分类
系统科学 | 31篇 |
丛书文集 | 57篇 |
教育与普及 | 4篇 |
理论与方法论 | 19篇 |
现状及发展 | 2618篇 |
研究方法 | 264篇 |
综合类 | 2303篇 |
自然研究 | 69篇 |
出版年
2017年 | 34篇 |
2012年 | 77篇 |
2011年 | 152篇 |
2008年 | 110篇 |
2007年 | 131篇 |
2006年 | 116篇 |
2005年 | 105篇 |
2004年 | 109篇 |
2003年 | 93篇 |
2002年 | 112篇 |
2001年 | 156篇 |
2000年 | 173篇 |
1999年 | 104篇 |
1992年 | 91篇 |
1991年 | 71篇 |
1990年 | 70篇 |
1989年 | 64篇 |
1988年 | 51篇 |
1987年 | 70篇 |
1986年 | 69篇 |
1985年 | 127篇 |
1984年 | 76篇 |
1983年 | 68篇 |
1982年 | 60篇 |
1981年 | 61篇 |
1980年 | 69篇 |
1979年 | 132篇 |
1978年 | 124篇 |
1977年 | 133篇 |
1976年 | 121篇 |
1975年 | 134篇 |
1974年 | 145篇 |
1973年 | 110篇 |
1972年 | 102篇 |
1971年 | 149篇 |
1970年 | 229篇 |
1969年 | 151篇 |
1968年 | 138篇 |
1967年 | 163篇 |
1966年 | 124篇 |
1965年 | 82篇 |
1964年 | 48篇 |
1962年 | 37篇 |
1959年 | 67篇 |
1958年 | 82篇 |
1957年 | 81篇 |
1956年 | 52篇 |
1955年 | 48篇 |
1954年 | 54篇 |
1948年 | 41篇 |
排序方式: 共有5365条查询结果,搜索用时 15 毫秒
11.
Short alanine peptides, containing 16 or 17 residues, appear to form alpha-helices in aqueous solution. But the main spectroscopic analyses used on helical peptides (circular dichroism and nuclear magnetic resonance) cannot distinguish between an alpha-helix (in which the ith residue is hydrogen-bonded to residue i + 4; ref. 9) and the next most common peptide helix, the 3(10)-helix10 (i-->i + 3 hydrogen-bonding). To address this problem we have designed single and doubly spin-labelled analogues of alanine-based peptides in which the nitroxide spin label forms an unbranched side chain extending from the sulphur atom of a cysteine residue. Here we report the circular dichroism, Fourier-transform infrared and electron-spin resonance spectra of these peptides under helix-forming conditions. The infrared absorbance gives an amide I' band with a frequency that is substantially different from that observed for alpha-helices. The electron-spin resonance spectra of doubly labelled helices show that the ranking of distances between side chains, around a single turn (residues 4-8), is inconsistent with an alpha-helical structure. Our experiments suggest that the more likely peptide geometry is a 3(10)-helix. 相似文献
12.
Ana Cuesta Laia Pedrola Teresa Sevilla Javier García-Planells María José Chumillas Fernando Mayordomo Eric LeGuern Ignacio Marín Juan J Vílchez Francesc Palau 《Nature genetics》2002,30(1):22-25
We identified three distinct mutations and six mutant alleles in GDAP1 in three families with axonal Charcot-Marie-Tooth (CMT) neuropathy and vocal cord paresis, which were previously linked to the CMT4A locus on chromosome 8q21.1. These results establish the molecular etiology of CMT4A (MIM 214400) and suggest that it may be associated with both axonal and demyelinating phenotypes. 相似文献
13.
Coalignment of vimentin intermediate filaments with microtubules depends on kinesin. 总被引:19,自引:0,他引:19
Intermediate filaments in most types of cultured cells coalign with microtubules. Depolymerization of microtubules results in collapse of vimentin and desmin intermediate filaments to the nucleus where they form a perinuclear cap. Collapse can also be induced by microinjection of antibodies against intermediate filament or microtubule proteins. Thus, two filament systems interact with each other. But the molecules mediating this interaction are unknown. One of the candidates for this role is a microtubule motor kinesin. Recent data showed that kinesin is involved in the plus end-directed movement of the membranous organelles along microtubules such as radial extension of lysosomes in macrophages and centrifugal movement of pigment in melanophores. Here we report that injection of the anti-kinesin antibody into human fibroblasts results in the redistribution of intermediate filaments to a tight perinuclear aggregate but had no effect on the distribution of microtubules. Thus, kinesin is involved not only in organelle movement but also in interaction of the two major cytoskeletal systems, intermediate filaments and microtubules. 相似文献
14.
15.
16.
17.
The effects of alcohol consumption, cigarette smoking and age on sister chromatid exchange (SCE) frequency in human lymphocytes were assessed by means of multiple linear regression. An increase in SCE rates was associated with alcohol consumption (p = 0.0001), smoking (p = 0.0231), and, to a small extent (p = 0.057), age. These three confounding factors explain 48% of the inter-personal variation in SCE rates among subjects studied. 相似文献
18.
J A Martignetti A A Aqeel W A Sewairi C E Boumah M Kambouris S A Mayouf K V Sheth W A Eid O Dowling J Harris M J Glucksman S Bahabri B F Meyer R J Desnick 《Nature genetics》2001,28(3):261-265
The inherited osteolyses or 'vanishing bone' syndromes are a group of rare disorders of unknown etiology characterized by destruction and resorption of affected bones. The multicentric osteolyses are notable for interphalangeal joint erosions that mimic severe juvenile rheumatoid arthritis (OMIMs 166300, 259600, 259610 and 277950). We recently described an autosomal recessive form of multicentric osteolysis with carpal and tarsal resorption, crippling arthritic changes, marked osteoporosis, palmar and plantar subcutaneous nodules and distinctive facies in a number of consanguineous Saudi Arabian families. We localized the disease gene to 16q12-21 by using members of these families for a genome-wide search for homozygous-by-descent microsatellite markers. Haplotype analysis narrowed the critical region to a 1.2-cM region that spans the gene encoding MMP-2 (gelatinase A, collagenase type IV; (ref. 3). We detected no MMP2 enzymatic activity in the serum or fibroblasts of affected family members. We identified two family-specific homoallelic MMP2 mutations: R101H and Y244X. The nonsense mutation effects a deletion of the substrate-binding and catalytic sites and the fibronectin type II-like and hemopexin/TIMP2 binding domains. Based on molecular modeling, the missense mutation disrupts hydrogen bond formation within the highly conserved prodomain adjacent to the catalytic zinc ion. 相似文献
19.
V. Ralevic P. Milner K. A. Kirkpatrick G. Burnstock 《Cellular and molecular life sciences : CMLS》1992,48(1):31-34
Adenosine 5-triphosphate (ATP) was released into the perfusate of rat isolated mesenteric arterial beds during each of two consecutive increases in flow. There was no significant difference between the amounts of ATP released on each occasion. Substance P was also released into the perfusate by increased flow, although its release was more variable. Removal of the endothelium of the mesenteric vessels with sodium deoxycholate led to a significant reduction (74%) in the amount of ATP released compared with the release before the endothelium had been removed. This suggests that the ATP released into the mesenteric arterial perfusate during increased flow arises from endothelial cells. 相似文献
20.
A. M. Landolt V. Osterwalder T. A. Landolt 《Cellular and molecular life sciences : CMLS》1985,41(5):640-642
Summary Adenomatous prolactin cells lose 39% of their cytoplasm volume within 7 days after the beginning of bromocriptine treatment. A simultaneous reduction of the rough-surfaced endoplasmic reticulum and the Golgi apparatus occurs. Their membranes are removed by rapid transport along the secretory pathway to the cell surface and to lysosomal destruction.We thank Prof. E. del Pozo, Sandoz Ltd, Basle, for the generous gift of injectable bromocriptine, Prof. E.R. Weibel, Dept. of Anatomy, University of Bern, for his technical advice, Dr U. Helfenstein, Institute of Biostatistics, University of Zürich, for his help in the statistical evaluation of the results and the EMDO-Foundation, Zürich, for financial support. 相似文献