The SARS-CoV S glycoprotein

The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. Recent rapid advances in our knowledge of the structure and function of this protein have lead to the development of a number of candidate vaccine immunogens and SARS-CoV entry inhibitors.     

A membrane-targeting signal in the amino terminus of the neuronal protein GAP-43

Neurons and other cells, such as those of epithelia, accumulate particular proteins in spatially discrete domains of the plasma membrane. This enrichment is probably important for localization of function, but it is not clear how it is accomplished. One proposal for epithelial cells is that proteins contain targeting signals which guide preferential accumulation in basal or apical membranes. The growth-cone membrane of a neuron serves as a specialized transduction system, which helps to convert cues from its environment into regulated growth. Because it can be physically separated from the cell soma, it has been possible to show that the growth-cone membrane contains a restricted set of total cellular proteins, although, to our knowledge, no proteins are limited to that structure. One of the most prominent proteins in the growth-cone membrane is GAP-43. Basi et al. have suggested that the N-terminus of GAP-43 might be important for the binding of GAP-43 to the growth-cone membrane. Skene and Virag recently found that the cysteines in the N-terminus are fatty-acylated and that this post-translational modification correlates with membrane-binding ability. We investigated the binding of GAP-43 to the growth-cone membrane by mutational analysis and by laser-scanning confocal microscopy of fusion proteins that included regions of GAP-43 and chloramphenicol acetyltransferase (CAT). We found that a short stretch of the GAP-43 N-terminus suffices to direct accumulation in growth-cone membranes, especially in the filopodia. This supports a previous proposal for the importance of this region of GAP-43 in determining the membrane distribution of GAP-43.     

浅述网络技术与办公自动化

随着网络信息技术的迅速发展和我国国民经济信息化建设进程的加快，办公自动化系统(OA)作为信息化建设的重点之一，越来越显示出其重要性。对办公自动化系统的发展方向，办公自动化软件的开发平台以及开发过程中经常遇到的具有共性的问题进行了分析，并提出了解决问题的可行性方案。     

Heat shock proteins in autoimmune disease. From causative antigen to specific therapy?

Heat shock proteins (hsp) are highly conserved from bacteria to man. Bacterial hsp, with approximate molecular weights of 60 kDa (hsp60), are immunodominant antigens that are immunologically cross-reactive with their mammalian counterparts. Hsp molecules are therefore useful in studies of fundamental questions concerning immune responses to foreign as opposed to self antigens. The finding that immune responses to hsp are associated with both experimentally-induced and spontaneous autoimmune diseases in animals has prompted intensive research to assess the role of bacterial hsp as the etiological agents involved in the development of autoimmune diseases. Recent evidence from animal models of autoimmune disease has clearly demonstrated the involvement of hsp in both the pathogenesis and the immunoregulation of autoimmune diseases. Studies with arthritogenic and diabetogenic T cell clones have identified immunogenic epitopes of hsp. These have been shown to ameliorate adjuvant arthritis in Lewis rats, and insulin-dependent diabetes mellitus (IDDM) in non-obese diabetic (NOD) mice. Such studies may have important therapeutic implications for the future treatment of human autoimmune disease.     

用热致液晶检测纤维增强复合材料的裂纹扩展

报告了用热致液晶对玻璃纤维增强环氧树脂材料表面裂纹疲劳过程进行的检测，成功地给出了裂纹周围的温度场分布．     

一种二维Walsh－Hadamard变换的快速算法

首先研究了二维Ｗａｌｓｈ－Ｈａｄａｍａｒｄ变换，利用Ｈ＿Ｎ矩阵的特性，推出了以单元算法为核心的快速算法。该方法在图象传输技术诸方面的应用可大大提高图象的传输速度。     

Experimental earth tidal models in considering nearly diurnal free wobble of the Earth''''s liquid core

Based on the 28 series of the high precision and high minute sampling tidal gravity observations at 20 stations in Global Geodynamics Project (GGP) network, the resonant parameters of the Earth's nearly diurnal free wobble (including the eigenperiods, resonant strengths and quality factots) are precisely determined. The discrepancy of the eigenperiod between observed and theoretical values is studied, the important conclusion that the real dynamic ellipticity of the liquid core is about 5% larger than the one under the static equilibrium assumption is approved by using our gravity technique. The experimental Earth's tidal gravity models with considering the nearly diurnal free wobble of the Earth's liquid core are constructed in this study. The numerical results show that the difference among three experimental models is less than 0.1%, and the largest discrepancy compared to those widely used nowdays given by Dehant (1999) and Mathews (2001) is only about 0.4%. It can provide with the most recent real     

Effect of light on variations in blood testosterone in the ram: demonstration of a photosensitive phase in diurnal rhythm]

6 groups each of 5 adult rams were subjected in light controlled pens to a pretreatment of normal variations in daylength during May and June. Then one group followed the normal daylength and the other five different "skeleton photoperiods" of 8 hrs of light per day consisting of: 7 hrs continuous light (7 L) + 1 hr light (1 L) situated at different times of the dark period (D) according to groups. Blood samples were taken once each week and hourly during one 24 hr period in June, July and September. Peripheral plasma testosterone measured by radio-immunoassay was used as an index of testicular activity. There was increase in the level of testosterone resuting from a rise in the number of peak release during the 24 hrs. In the different light treatments tested, only the treatment (7L+9D+1L)+7D) stimulated testosterone secretion with the pattern of testosterone of this group being similar to that of the control group. This indicates that there exists a photosensitive phase at 16 to 17 hrs after the beginning of the principal light (the subjective dawn).     

24份龙眼种质资源RAPD分析

利用RAPD技术对原产福建20份、广东1份、四川3份,共24份龙眼种质资源进行分析.从100条引物中筛选出14条多态性引物,共扩增出122条谱带,多态性谱带共88条,多态性比例72.1%.对RAPD结果进行聚类分析,根据相似系数0.28的水平可将24份龙眼品种(系)分为3类.供试各品种多态性比例介于0.12和0.28之间,揭示福建本地品种的遗传多样性不太丰富.