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71.
A defined range of guard cell calcium oscillation parameters encodes stomatal movements. 总被引:59,自引:0,他引:59
G J Allen S P Chu C L Harrington K Schumacher T Hoffmann Y Y Tang E Grill J I Schroeder 《Nature》2001,411(6841):1053-1057
Oscillations in cytosolic calcium concentration ([Ca2+]cyt) are central regulators of signal transduction cascades, although the roles of individual [Ca2+]cyt oscillation parameters in regulating downstream physiological responses remain largely unknown. In plants, guard cells integrate environmental and endogenous signals to regulate the aperture of stomatal pores and [Ca2+]cyt oscillations are a fundamental component of stomatal closure. Here we systematically vary [Ca2+]cyt oscillation parameters in Arabidopsis guard cells using a 'calcium clamp' and show that [Ca2+]cyt controls stomatal closure by two mechanisms. Short-term 'calcium-reactive' closure occurred rapidly when [Ca2+]cyt was elevated, whereas the degree of long-term steady-state closure was 'calcium programmed' by [Ca2+]cyt oscillations within a defined range of frequency, transient number, duration and amplitude. Furthermore, in guard cells of the gca2 mutant, [Ca2+]cyt oscillations induced by abscisic acid and extracellular calcium had increased frequencies and reduced transient duration, and steady-state stomatal closure was abolished. Experimentally imposing [Ca2+]cyt oscillations with parameters that elicited closure in the wild type restored long-term closure in gca2 stomata. These data show that a defined window of guard cell [Ca2+]cyt oscillation parameters programs changes in steady-state stomatal aperture. 相似文献
72.
73.
Krishnan KJ Reeve AK Samuels DC Chinnery PF Blackwood JK Taylor RW Wanrooij S Spelbrink JN Lightowlers RN Turnbull DM 《Nature genetics》2008,40(3):275-279
Mitochondrial DNA (mtDNA) deletions are a primary cause of mitochondrial disease and are likely to have a central role in the aging of postmitotic tissues. Understanding the mechanism of the formation and subsequent clonal expansion of these mtDNA deletions is an essential first step in trying to prevent their occurrence. We review the previous literature and recent results from our own laboratories, and conclude that mtDNA deletions are most likely to occur during repair of damaged mtDNA rather than during replication. This conclusion has important implications for prevention of mtDNA disease and, potentially, for our understanding of the aging process. 相似文献
74.
The influence of tree species on soils has received much attention,but we know little about either the pattern and underlying mechanisms of net nutrient and carbon inputs under canopy for various tree species or the effects of net nutrient input on soil nutrient contents.To address these issues,we selected three tree species with distinct leaf sizes and arranged litter traps around the target individuals to test what affected net nutrient and carbon inputs under canopy of target individuals,and how net nutrient inputs influenced soil nutrient contents.The results showed that net litter manganese(Mn)and lignin inputs were significantly different among these tree species,as well as soil exchangeable potassium(K),available iron(Fe)and available Mn.The results also revealed that the species with the smallest sized leaves may stimulate more net Mn and lignin inputs than the species with the largest sized leaves.At the same time,net nutrient inputs correlated with soil available Fe,while the ratio of lignin to nitrogen of net litter correlated with total phosphorus contents.These results demonstrated that litter production may be the main factor deciding net nutrient and carbon inputs under different tree species’canopy,which significantly differentiated soil iron’s availability. 相似文献
75.
自来水厂污泥的处理和利用是一个至今没有得到完全解决的问题,通常的做法是将其直接排放或脱水后填埋.在实验研究的基础上,论述了采用工业石膏(CaSO4·2H2O)和有机聚合物对自来水厂污泥进行双重调解进而脱水的方法.实验结果表明,用11(工业石膏和有机聚合物的重量比)的投量对自来水厂污泥进行双重调解可明显改善污泥的过滤性能.脱水实验证明双重调解后的污泥经机械脱水,其泥饼含水率可比单纯有机聚合物调解后脱水的泥饼含水率低大约7个百分点.此项研究的意义在于探索自来水厂污泥脱水后农用及作为人工湿地填料的可能性,同时也为自来水厂污泥脱水后的最终处置探索新思路. 相似文献
76.
Saliba KJ Martin RE Bröer A Henry RI McCarthy CS Downie MJ Allen RJ Mullin KA McFadden GI Bröer S Kirk K 《Nature》2006,443(7111):582-585
As the malaria parasite, Plasmodium falciparum, grows within its host erythrocyte it induces an increase in the permeability of the erythrocyte membrane to a range of low-molecular-mass solutes, including Na+ and K+ (ref. 1). This results in a progressive increase in the concentration of Na+ in the erythrocyte cytosol. The parasite cytosol has a relatively low Na+ concentration and there is therefore a large inward Na+ gradient across the parasite plasma membrane. Here we show that the parasite exploits the Na+ electrochemical gradient to energize the uptake of inorganic phosphate (P(i)), an essential nutrient. P(i) was taken up into the intracellular parasite by a Na+-dependent transporter, with a stoichiometry of 2Na+:1P(i) and with an apparent preference for the monovalent over the divalent form of P(i). A P(i) transporter (PfPiT) belonging to the PiT family was cloned from the parasite and localized to the parasite surface. Expression of PfPiT in Xenopus oocytes resulted in Na+-dependent P(i) uptake with characteristics similar to those observed for P(i) uptake in the parasite. This study provides new insight into the significance of the malaria-parasite-induced alteration of the ionic composition of its host cell. 相似文献
77.
Nusbaum C Mikkelsen TS Zody MC Asakawa S Taudien S Garber M Kodira CD Schueler MG Shimizu A Whittaker CA Chang JL Cuomo CA Dewar K FitzGerald MG Yang X Allen NR Anderson S Asakawa T Blechschmidt K Bloom T Borowsky ML Butler J Cook A Corum B DeArellano K DeCaprio D Dooley KT Dorris L Engels R Glöckner G Hafez N Hagopian DS Hall JL Ishikawa SK Jaffe DB Kamat A Kudoh J Lehmann R Lokitsang T Macdonald P Major JE Matthews CD Mauceli E Menzel U Mihalev AH Minoshima S Murayama Y Naylor JW Nicol R 《Nature》2006,439(7074):331-335
The International Human Genome Sequencing Consortium (IHGSC) recently completed a sequence of the human genome. As part of this project, we have focused on chromosome 8. Although some chromosomes exhibit extreme characteristics in terms of length, gene content, repeat content and fraction segmentally duplicated, chromosome 8 is distinctly typical in character, being very close to the genome median in each of these aspects. This work describes a finished sequence and gene catalogue for the chromosome, which represents just over 5% of the euchromatic human genome. A unique feature of the chromosome is a vast region of approximately 15 megabases on distal 8p that appears to have a strikingly high mutation rate, which has accelerated in the hominids relative to other sequenced mammals. This fast-evolving region contains a number of genes related to innate immunity and the nervous system, including loci that appear to be under positive selection--these include the major defensin (DEF) gene cluster and MCPH1, a gene that may have contributed to the evolution of expanded brain size in the great apes. The data from chromosome 8 should allow a better understanding of both normal and disease biology and genome evolution. 相似文献
78.
Bazooka recruits Inscuteable to orient asymmetric cell divisions in Drosophila neuroblasts 总被引:3,自引:0,他引:3
Asymmetric cell divisions can be generated by the segregation of determinants into one of the two daughter cells. In Drosophila, neuroblasts divide asymmetrically along the apical-basal axis shortly after their delamination from the neuroectodermal epithelium. Several proteins, including Numb and Miranda, segregate into the basal daughter cell and are needed for the determination of its correct cell fate. Both the apical-basal orientation of the mitotic spindle and the localization of Numb and Miranda to the basal cell cortex are directed by Inscuteable, a protein that localizes to the apical cell cortex before and during neuroblast mitosis. Here we show that the apical localizaton of Inscuteable requires Bazooka, a protein containing a PDZ domain that is essential for apical-basal polarity in epithelial cells. Bazooka localizes with Inscuteable in neuroblasts and binds to the Inscuteable localization domain in vitro and in vivo. In embryos lacking both maternal and zygotic bazooka function, Inscuteable no longer localizes asymmetrically in neuroblasts and is instead uniformly distributed in the cytoplasm. Mitotic spindles in neuroblasts are misoriented in these embryos, and the proteins Numb and Miranda fail to localize asymmetrically in metaphase. Our results suggest that direct binding to Bazooka mediates the asymmetric localization of Inscuteable and connects the asymmetric division of neuroblasts to the axis of epithelial apical-basal polarity. 相似文献
79.
80.
Evolution of microRNA genes by inverted duplication of target gene sequences in Arabidopsis thaliana 总被引:8,自引:0,他引:8
Allen E Xie Z Gustafson AM Sung GH Spatafora JW Carrington JC 《Nature genetics》2004,36(12):1282-1290