Cloning and expressional analyses of a cinnamoyl CoA reductase cDNA from rice seedlings

Cinnamoyl CoA reductase (CCR: EC 1.2.1.44),the entry-point enzyme of the llgnin specific biosynthetic pathway, catalyzes the conversion of cinnamoyl CoA esters to their corresponding dnnamaldehydes. Multiple sequence alignment showed that the deduced polypeptide shared 70% similarity and 30% sequence identity at the amino acid level with defined CCR genes from other plant species and they all contain the common signature sequences thought to be the catalytic site as well as the putative NADP binding domain.Using a conserved OsCCR cDNA fragment as the probe for library screening, we isolated the genomic DNA that covered the whole coding region of OsCCR with total length of 3045bp including 4 introns and 5 exons. The open reading frame for our OsCCR gene coBtAin~ 337 amino adds. Northern blot indicated that OsCCR was expressed in different organs with the highest level found in stems. In situ hybridization results showed that OsCCR mRNA was localized mainly along the vascular bundles in stems and leaves, and also in lateral roots that was differentiating from the tiilering node. We conclude that the vascular-localized expression of OsCCR gene may suggest its possible involvement in llgnin biosynthesis. Cloning and characterization of OsCCR will help to clarify how llgniflcations in plants are regulated and will provide a physical basis for creating genetically engineered rice plants with optimal lignin contents.     

浅谈建筑节能

论述了在进行建筑的结构设计时要充分考虑其节能功能,阐述了建筑节能的技术与应用.     

浅谈建筑节能

论述了在进行建筑的结构设计时要充分考虑其节能功能,阐述了建筑节能的技术与应用。     

空心板桥水泥混凝土桥面铺装结构有限元分析

运用ANSYS软件对空心板桥水泥混凝土铺装层进行实体建模,并针对铺装层破坏形式开展力学分析,提出铺装层破坏的解决办法.     

瓦斯变化规律引发的瓦斯防治办法探讨

文章对朔州市煤矿中存在的诸多问题和瓦斯变化规律进行了分析,总结出一套瓦斯防治的办法.     

交际策略与英语口语教学

文章对于教学中如何通过课堂教学提高学生的交际策略能力，及其在口语课中的具体课堂教学，进行了初步的探讨。     

浅谈建筑节能

论述了在进行建筑的结构设计时要充分考虑其节能功能，阐述了建筑节能的技术与应用。     

THE λ-GROBNER BASES UNDER POLYNOMIAL COMPOSITION

Polynomial composition is the operation of replacing variables in a polynomial with other polynomials. λ-Grgbner basis is an especial Grobner basis. The main problem in the paper is： when does composition commute with λ-Grobner basis computation？ We shall answer better the above question. This has a natural application in the computation of λ-Grobner bases.     

Modification of the rib operon derived from Bacillus subtilis and its expression in Escherichia coli

A riboflavin operon （rib operon） derived from Bacillus subtilis 368 was modified on structure and the resulting operons were expressed in various strains of Escherichia coli. The results showed that the optimization of the rib operon and the host strain used for expression are two main factors affecting the riboflavin production. Replacing the promoterl and rfn box of the rib operon with a strong constructive promoter spol drastically increased the expression of the rib genes. When E. coli JM109 was used as the host strain, the highest riboflavin production reached 95.3μg/mL （about eight times higher than that of the unmodified r/b operon）. In addition, when tetracycline （20 μg/mL） was used as the selective pressure, compared with the ampicillin resistant transformants, a higher riboflavin yield was obtained in tetracycline resistant host strain.     

非等温莫来石晶形成动力学研究

采用综合热分析技术,通过分析TG-DSC曲线,研究了矾土在空气气氛下莫来石晶化动力学过程.采用X射线衍射仪对试样进行了物相分析,经数据处理,结合Kissinger方程,得到了不同升温速率下莫来石晶化动力学的3指数：活化能E=853.608±2.15 kJ/mol,表观指前因子A=1.19×10^35s^-1,反应机理函数f（α）=（1-α）^1.09～1.26,生矾土矿的莫来石晶化温度为980～1 002 ℃,晶化反应为一级固相反应.