声参量混合定则预测生物组织组分的研究

从理想混合液模型出发，将生物组织看作是主要由水、脂肪、蛋白质所构成的三元理想系统．测量了蛋白、蛋黄及其混合物的声速、密度和非线性声参量Ｂ／Ａ，并根据修正后的Ａｐｆｅｌ混合定则，对该混合物进行水分、脂肪和蛋白质体积含量预测．结果表明，实测体积含量与Ａｐｆｅｌ混合定则预测值吻合较好     

回转类零件自由曲线CAD／CAM集成的实现

运用面向对象技术，研究了回转类零件中自由曲线ＣＡＤ／ＣＡＭ集成方法。     

一种改进的模糊综合评判模型

本文在对模糊综合评判模型进行分析的基础上，引进了一种广义模糊算子，得到广义算子下的模糊综合评判模型。并给出这种模型在学生量化管理中的应用。由于信息利用率较高，评判结果较为精细，从而有铲地克服了传统的模糊综合知识更新的判模型存在的缺陷。     

黑曲霉胞外葡萄糖淀粉酶的纯化及特性

黑曲霉（Ａｓｐｅｒｇｉｌｌｕｓｎｉｇｅｒ７２８）的粗酶液，经硫酸铵沉淀，ＳｅｐｈａｄｅｘＧ－２５柱凝胶过滤脱盐，ＤＥＡＥ－Ｔｏｙｏｐｅａｒｌ离子交换柱层析和ＳｅｐｈａｃｒｙＳ－１００凝胶层析纯化，经ＰＡＧＥ鉴定为一条带ＳＤＳ凝胶电泳测定分子量为７００００．金属离子Ｆｅ３＋对该酶有一定的抑制作用，该酶的等电点为３．７，最适ｐＨ为４，最适温度为６０℃．Ｅ２８０１％为１５．７２，Ｋｍ值为０．１１２×１０－３ｍ．     

四环素产生菌质粒DNA的分离检测及遗传学分析

用７％蔗糖的ＹＥＭＥ液体培养基培养四环素产生菌金色链霉菌（Ｓｔｒｅｐｔｏｍｙｃｅｓ ａｕｒｅｏｆａｃｉｅｎｓ）９１－０６，以链霉菌遣传操作法为基础，从该菌体中分离到质粒ＰＳＡ３１４，分子量约为２８．５ｋｂ（１８．８ＭＤ）。该质粒经酶切分析表明，具有２个ＢａｍＨＩ位点和１个ＥｃｏＲＩ位点，用吖啶橙处理获得无质粒珠，其在孢子形成和菌落形态方面出现明显差异。     

高圆双折射光纤的分析与设计

本文对高圆双折射光纤的偏振特性作了分析，提出了一个能确定高圆双折射光纤保偏性能的特性参数．文中还给出了对线偏振光输入、出射光消光比满足一定设计值下高圆双折射光纤固有线双折射及弯曲半径与光纤扭转率之间的关系曲线．这些结果为这类光纤的设计及性能判定提供了理论依据．     

建筑火灾综合模拟评估在大空间建筑火灾中的初步应用

运用火灾规律双重性理论，探讨建筑火灾综合模拟评估的理论框架，以影院会堂类大空间建筑为例，运用一些可调试的模拟方法、统计分析及其模型，给出在火灾评估具体环节和应用程式上的一定程度的量化描述．实现火灾综合模拟评估理论模型的可操作性，使更进一步准确和方便地估算建筑火灾对人员、财产造成危害的几率，和判断这种危害的发生具备了初步的可能．     

Protein aggregation as primary and characteristic cell reaction to various stresses

Ehrlich carcinoma and EL-4 thymoma ascites cells were subjected in vitro to heat shock, ATP depletion, oxidative stress, Ca²⁺ overlading and iodoacetamide treatment. After the transient stresses, Triton (X-100)-insoluble TIS) fractions were isolated from the cells and analysed by electrophoresis and immunoblotting. All stresses used caused rapid aggregation of cell proteins. This was manifested in a signficant rise in protein content in the TIS fractions. The protein increase was mostly due to and increase in the insolubility of actin, 57 kDa protein of intermediate filaments, 70 kDa heat shock protein (HSP 70), and some specific proteins whose insolubilization was a characteristic sign for each type of cell injury. Different survival rates in the cell lines after either stress corrlated well with differences in their TIS protein accretion. Possible mechanisms for stress-induced protein aggregation and its relationship with cell viability are suggested.     

Effects of corpora cardiaca extract,furosemide and ion substitution on sodium and chloride flux in perfused Malpighian tubules ofLocusta

Treatment with the co-transport inhibitor, furosemide decreased³⁶Cl^– flux across perfused Malpighian tubules ofLocusta. However, exclusion of³⁶Cl^– from the bathing medium had not effect on²²Na⁺ flux whereas substitution of bathing medium Na⁺ by K⁺ increased³⁶Cl^– flux. Diuretic extract of corpora cardiaca increased²²Na⁺ (by 106%) and³⁶Cl^– (by 335%) fluxes differentially.