区域环境决策的协调支持模式

为使区域环境决策更加有效化，文中建议开发和设计区域环境决策支持系统，定义了系统协调思想和总体结构模式，提出了系统协调开发新方法，分析了该系统的功能组成，探索出一条有效开发ＤＳＳ的新思路．     

BICP0 and its RING finger domain act as ubiquitin E3 ligases <Emphasis Type="Italic">in vitro</Emphasis>

Bovine infected-cell protein 0 (BICP0) encoded by bovine herpes virus 1 (BHV-1) immediate early gene is necessary for efficient productive infection, in a large part,because it activates all 3 classes of BHV-1 genes. It also has the ability to efficiently transactivate promoters that are not derived from BHV-1. To investigate the mechanism by which BICP0 achieves these effects, we expressed and purified BICP0 and its different mutants in E. coil In vitro assays showed that both full-length BICP0 and its isolated RING finger domain induce the accumulation of polyubiquitin chains. Mutations within the RING finger region that abolish the in vitro ubiquitination activity also cause severe reductions in BICP0 activity in other assays. Based on these, we conclude that BICP0 has the potential to act as an E3 ubiquitin ligase during viral infection and its RING finger domain is necessary for this function. These strongly support the hypothesis that BICPO might influence virus infection through its ability to interact with the ubiquitin-proteasome pathway.     

PTES专家系统知识库的构造

本文大专家系统设计方法的指导下，通过对整理、知识表示、知示获取、知识库构造等环节的描述，介绍了一个植物分类专家系统知识库的构造方法     

几种羟肟酸捕收剂与白云石作用机理研究

为了更好的揭示羟肟酸类捕收剂与白云石作用机理,利用Materials Studio(MS)软件建立羟肟酸捕收剂在白云石矿物表面的吸附模型,通过分子模拟技术模拟计算了真空环境下药剂分子与白云石矿物的相互作用能。从原子尺度揭示了捕收剂在白云石浮选中的捕收机理,研究表明吸附能的差异是导致捕收剂选择性捕收的根本原因。     

对机器人平面执行机构动力学方程的探讨

文章以开式链平面连杆机构为对象,从新的角度探索分析其动力学方程的建立问题,文中依次对类速度,类角速度,惯性系数,广义力给出新的求解通式,最后用拉格朗日方程建立相应的动力学方程。     

微翅管几何参数对混合工质流动沸腾特性的影响

对非共沸混合工质R32／R134a在水平微翅管内的流动沸腾特性进行了实验研究,获得了微翅管的翅高和翅数对换热性能的影响,并综合比较了3根微翅管的换热性能。同时,将微翅管与光管性能进行了比较。结果表明,在相同工况下,翅高为0．2mm、翅数为60的换热性能最好;微翅管的换热性能明显优于光管。     

A study on the effect of the bacterial inoculant on corn silage quality,digestibility and performance in dairy cattle

Effect of the bacterial inoculant Sil-All (short for ‘ SA‘ ) on corn silage quality, digestibility and performance in dairy cattle was studied. SA treated silage resulted in the higher level of retained protein and slightly higher level of residual sugars in that treatment, the better digestibility of the neutral detergent fibre (NDF) and the acid detergent fibre (ADF), the lower pH and evolution of ammonia compared to the control; The fall in pH was most rapid in the SA treated silage in the first 4 days of ensilage, when it matters most for the conservation of protein, compared with the control silage and this was linked more to both lactic and total acids. Both volatile fatty acid (VFA) and lactate content increased with ensiling time, increase degree reached up near 100%; Lactate content in treatment silage was higher than that in the control, and this difference was all maintained at the level of approximately 7% - 13% almost in all cases, at length, up 16% in the case of day 15; pH value decreased with ensiling time. Moreover, pH value in most of treatment were lower than that in the control ; The digestibility of both dry matter (DM) and crude protein (CP) was appreciably improved by the use of inoculant SA. Also, in relation to reductions, of NDF and ADF were noted. An increase in milk yield of the order of 0.9kg per head per day in favour of SA was observed. Alternatively, with an average daily feed intake of 20kg, this indicates with 1 ton of corn silage milk yield could be increased by 46.5kg.     

大豆花瓣蛋白双向电泳分析技术体系的优化

双向电泳技术体系的优化是蛋白质组学研究的前提和基础.本文针对大豆花瓣含有大量色素和酚等干扰物质的现象,比较6种蛋白提取方法和2种蛋白裂解液配方,优化胶条范围和等电聚焦程序.结果表明:pH为4～7 IPG胶条适于大豆全花蛋白分离;TCA/丙酮+2D Clean Up试剂盒法去除杂质充分,提取的蛋白含量高、纯度好、分离效果佳、耗时短;蛋白裂解液采用5 mol/L尿素,2 M硫脲,2%CHAPS,2%SB3-10,5 mmol/L TCEP,20 mmol/L DTT,0.5%IPG Buffer为好;增加离心转速、时间和丙酮清洗次数能促进蛋白溶解,增加蛋白纯度,初步建立了一套适合大豆全花蛋白双向电泳分析的技术体系.     

不烧低碳滑板中温性能研究

通过分析不烧低碳滑板中温性能较低产生的原因,在其他学者工作的基础上,尝试通过引入白炭黑、含碳树脂粉、聚甲基硅树脂等结合剂或添加剂,开展实验室工作,以改善不烧低碳滑板的中温强度较低导致使用风险增加的问题.通过试验和数据分析,采用复合树脂为结合剂,可使试样中温强度达到常温耐压强度水平,同时提高了试样的抗氧化性,较为明显地改善了不烧低碳滑板中温强度较低的状况.     

大重型静压支承静态性能及油膜流体仿真

为了解决大重型数控转台的单油腔静压推力轴承无法承受偏载荷问题,提出了单油腔静压支承与静压径向轴承复合设计方案。同时,为了方便静压径向轴承油腔加工,提出了一种新式回型槽油腔结构。此种结构的油腔加工区域仅占单油腔总面积的10%,而承载区域达到75%以上。然后,综合应用三维造型软件Pro/ENGINEER和CFD软件FLUENT,模拟分析圆环形静压支承和回型槽油腔的流速分布及压力分布,揭示了两种油腔结构内部油液流动规律,并计算各自的静态性能,实验验证表明,该设计方案正确性与合理性。