全文获取类型
收费全文 | 253篇 |
免费 | 2篇 |
国内免费 | 1篇 |
专业分类
系统科学 | 4篇 |
教育与普及 | 1篇 |
理论与方法论 | 2篇 |
现状及发展 | 17篇 |
研究方法 | 18篇 |
综合类 | 177篇 |
自然研究 | 37篇 |
出版年
2022年 | 1篇 |
2020年 | 1篇 |
2018年 | 2篇 |
2015年 | 1篇 |
2013年 | 5篇 |
2012年 | 11篇 |
2011年 | 55篇 |
2010年 | 2篇 |
2009年 | 3篇 |
2008年 | 9篇 |
2007年 | 13篇 |
2006年 | 18篇 |
2005年 | 27篇 |
2004年 | 25篇 |
2003年 | 26篇 |
2002年 | 25篇 |
2001年 | 9篇 |
2000年 | 3篇 |
1999年 | 2篇 |
1998年 | 6篇 |
1996年 | 1篇 |
1988年 | 2篇 |
1984年 | 2篇 |
1982年 | 1篇 |
1977年 | 1篇 |
1976年 | 1篇 |
1971年 | 1篇 |
1967年 | 1篇 |
1966年 | 1篇 |
1965年 | 1篇 |
排序方式: 共有256条查询结果,搜索用时 296 毫秒
231.
Gilman AG Simon MI Bourne HR Harris BA Long R Ross EM Stull JT Taussig R Bourne HR Arkin AP Cobb MH Cyster JG Devreotes PN Ferrell JE Fruman D Gold M Weiss A Stull JT Berridge MJ Cantley LC Catterall WA Coughlin SR Olson EN Smith TF Brugge JS Botstein D Dixon JE Hunter T Lefkowitz RJ Pawson AJ Sternberg PW Varmus H Subramaniam S Sinkovits RS Li J Mock D Ning Y Saunders B Sternweis PC Hilgemann D Scheuermann RH DeCamp D Hsueh R Lin KM Ni Y Seaman WE Simpson PC O'Connell TD Roach T Simon MI 《Nature》2002,420(6916):703-706
The Alliance for Cellular Signaling is a large-scale collaboration designed to answer global questions about signalling networks. Pathways will be studied intensively in two cells--B lymphocytes (the cells of the immune system) and cardiac myocytes--to facilitate quantitative modelling. One goal is to catalyse complementary research in individual laboratories; to facilitate this, all alliance data are freely available for use by the entire research community. 相似文献
232.
233.
Destructive interference may lead to complete cancellation when light waves travelling in different directions cross, and in three-dimensional space this occurs along lines that are vortices of electromagnetic energy flow. Here we confirm theoretical predictions by experimentally creating combinations of optical laser beams in which these dark threads form stable loops that are linked and knotted. 相似文献
234.
235.
236.
237.
IkappaB kinase-alpha (IKK-alpha) exhibits protein-kinase-dependent and -independent functions. Its kinase activity is required for lymphoid organogenesis and mammary gland development, whereas a kinase-independent activity is required for epidermal keratinocyte differentiation. In addition to failed epidermal differentiation, IKK-alpha-deficient mice exhibit abnormal skeletal and craniofacial morphogenesis. As similar defects are not exhibited by mice that experience systemic inhibition of NF-kappaB, we postulated that the morphogenetic defects in IKK-alpha-deficient mice are not caused by reduced NF-kappaB activity but instead are due to failed epidermal differentiation that disrupts proper epidermal-mesodermal interactions. We tested this hypothesis by introducing an epidermal-specific Ikka (also known as Chuk) transgene into IKK-alpha-deficient mice. Mice lacking IKK-alpha in all cell types including bone and cartilage, but not in basal epidermal keratinocytes, exhibit normal epidermal differentiation and skeletal morphology. Thus, epidermal differentiation is required for proper morphogenesis of mesodermally derived skeletal elements. One way by which IKK-alpha controls skeletal and craniofacial morphogenesis is by repressing expression of fibroblast growth factor (FGF) family members, such as FGF8, whose expression is specifically elevated in the limb bud ectoderm of IKK-alpha-deficient mice. 相似文献
238.
5-hydroxytryptamine type 3 (5-HT3) receptors are members of the Cys-loop receptor superfamily. Neurotransmitter binding in these proteins triggers the opening (gating) of an ion channel by means of an as-yet-uncharacterized conformational change. Here we show that a specific proline (Pro 8*), located at the apex of the loop between the second and third transmembrane helices (M2-M3), can link binding to gating through a cis-trans isomerization of the protein backbone. Using unnatural amino acid mutagenesis, a series of proline analogues with varying preference for the cis conformer was incorporated at the 8* position. Proline analogues that strongly favour the trans conformer produced non-functional channels. Among the functional mutants there was a strong correlation between the intrinsic cis-trans energy gap of the proline analogue and the activation of the channel, suggesting that cis-trans isomerization of this single proline provides the switch that interconverts the open and closed states of the channel. Consistent with this proposal, nuclear magnetic resonance studies on an M2-M3 loop peptide reveal two distinct, structured forms. Our results thus confirm the structure of the M2-M3 loop and the critical role of Pro 8* in the 5-HT3 receptor. In addition, they suggest that a molecular rearrangement at Pro 8* is the structural mechanism that opens the receptor pore. 相似文献
239.
240.